| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 42,478 | G→C | 33.7% | G207G (GGG→GGC) | fixC → | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 42,478 | 0 | G | C | 33.7% | 73.4 / 55.7 | 74 | G207G (GGG→GGC) | fixC | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base G (15/34); new base C (16/9); total (31/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
AAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCA > minE/42413‑42542 | aaGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAgggggtg > 1:2810832/1‑67 (MQ=255) aCTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCt > 1:678840/1‑67 (MQ=255) gTTACCGAAGTCGGTTATCGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGTTTg < 1:1857574/68‑1 (MQ=255) gTTACCGAAGTCGGTTATCGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGTTTg < 1:2369470/68‑1 (MQ=255) gTTACCGAAGTCGGTTATCGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGTTTg < 1:2386869/68‑1 (MQ=255) gTTACCGAAGTCGGTTATCGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGTTTg < 1:1571464/68‑1 (MQ=255) gTTACCGAAGTCGGTTATCGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGTTTg < 1:1456307/68‑1 (MQ=255) gTTACCGAAGTCGGTTATCGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGTTTg < 1:283923/68‑1 (MQ=255) gTTACCGAAGTCGGTTATCGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGTTTg < 1:287842/68‑1 (MQ=255) gTTACCGAAGTCGGTTATCGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGTTTg < 1:3322779/68‑1 (MQ=255) gTTACCGAAGTCGGTTATCGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGTTTg < 1:1015481/68‑1 (MQ=255) tACCGAAGTCGGTTATTGAAGACCGTTTTCAGTT‑CAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:2042639/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGCCCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg > 1:1343665/1‑69 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:760259/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:995611/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:1908487/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg > 1:2100548/1‑69 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:97842/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:2440583/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:2491638/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:2896220/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:2504328/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:253171/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:337803/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:2719946/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:3275238/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg > 1:2879295/1‑69 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:1784286/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:1082629/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:1296644/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:1413346/69‑1 (MQ=255) aCCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCggg < 1:1529207/69‑1 (MQ=255) aGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATcac < 1:2692605/69‑1 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGTTAATCAGGGCGCGGCTTGCCTGt > 1:2757929/2‑47 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGtt > 1:568608/2‑48 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGtt > 1:2684509/2‑48 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGtt > 1:1991177/2‑48 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGtt > 1:3077624/2‑48 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGtt > 1:2865087/2‑48 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGtt > 1:151093/2‑48 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGtt > 1:2512902/2‑48 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGtt > 1:1193470/2‑48 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGtt > 1:1629990/2‑48 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGtt > 1:608474/2‑48 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGtt > 1:1743165/2‑48 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGtt > 1:2362407/2‑48 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGt > 1:2248076/2‑47 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCAGGGCGCGGCTTGCCTGt > 1:846892/2‑47 (MQ=255) cgAAGACCGTTTTCAGTTGCAGGGTAATCACGGCGCGGCTTGCCTGtt > 1:1423408/2‑48 (MQ=255) gACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCt < 1:198464/68‑1 (MQ=255) gACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCt < 1:1429621/68‑1 (MQ=255) gACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCt < 1:3226076/68‑1 (MQ=255) gACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCt < 1:799446/68‑1 (MQ=255) gACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCt < 1:2045588/68‑1 (MQ=255) ttCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGggcg > 1:1005859/1‑69 (MQ=255) ttCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGggcg > 1:1831754/1‑69 (MQ=255) ttCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGggcg > 1:254182/1‑69 (MQ=255) ttCAGTTGAAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGggcg > 1:1755803/1‑69 (MQ=255) ggTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCtt < 1:141368/69‑1 (MQ=255) ggTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCtt < 1:2211731/69‑1 (MQ=255) tAATCAGGGGGCGGCTTGCCTGTTTGCGGGATcacccacc < 1:1023415/40‑1 (MQ=255) tAATCAGGGGGCGGCTTGCCTGTTTGCGGGATcacccacc < 1:1260078/40‑1 (MQ=255) tAATCAGGGGGCGGCTTGCCTGTTTGCGGGATcacccacc < 1:1121986/40‑1 (MQ=255) tAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCtt > 1:3089304/1‑67 (MQ=255) tAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCtt < 1:1771218/67‑1 (MQ=255) tAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTta < 1:2648518/69‑1 (MQ=255) taTCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTta < 1:237342/67‑1 (MQ=255) aTCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTtat > 1:1584948/1‑68 (MQ=255) aTCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTtat > 1:2272177/1‑68 (MQ=255) aTCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTtat > 1:1579653/1‑68 (MQ=255) aGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTta < 1:1912194/64‑1 (MQ=255) aGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTta > 1:816376/1‑64 (MQ=255) aGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTta < 1:2649783/64‑1 (MQ=255) aGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTta > 1:993633/1‑64 (MQ=255) aGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCa > 1:1396345/1‑69 (MQ=255) gggCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTta < 1:1366972/61‑1 (MQ=255) | AAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCA > minE/42413‑42542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |