| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 373,571 | T→C | 23.6% | L141L (TTG→CTG) | fepE → | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 373,571 | 0 | T | C | 23.6% | 145.8 / 38.7 | 114 | L141L (TTG→CTG) | fepE | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Reads supporting (aligned to +/- strand): ref base T (41/46); new base C (8/19); total (49/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 120 total aligned reads displayed.
AGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGCCAGTAAGAAAAAA > minE/373506‑373639 | agTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGc < 1:1027761/69‑1 (MQ=255)agTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGc < 1:1107463/69‑1 (MQ=255)agTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGc < 1:79417/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:2757906/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:1746939/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:1592560/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:2345649/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:101392/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:1454295/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:2769417/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:2803689/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:839557/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:469183/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:1195702/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:479373/69‑1 (MQ=255) gTACCTGCGTTCATCACCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCa < 1:1109901/69‑1 (MQ=255) tACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCAt > 1:1843938/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCAt > 1:2049714/1‑67 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2300291/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:1876394/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2050860/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2179367/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2277955/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:3262185/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2468898/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2662145/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:2426194/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:347173/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:3389758/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATcg > 1:1500968/1‑69 (MQ=255) ccTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATc > 1:1779859/1‑68 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:1317306/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc < 1:2648258/69‑1 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc < 1:2588065/69‑1 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:2685707/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc < 1:1349102/69‑1 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:2876191/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:288359/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc < 1:222350/69‑1 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:313586/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:2157912/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc < 1:3387097/69‑1 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:1687716/1‑69 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc < 1:1718465/69‑1 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc < 1:44285/69‑1 (MQ=255) gCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCgcgc > 1:484610/1‑69 (MQ=255) ttCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCa < 1:3123218/67‑1 (MQ=255) ttCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCa < 1:2732224/67‑1 (MQ=255) ttCATCACCTTATGCGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAAt < 1:186153/69‑1 (MQ=255) catcaCCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCATCGCGCCATTg > 1:472056/1‑69 (MQ=255) catcaCCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCATCGCGCCATTg > 1:2840410/1‑69 (MQ=255) catcaCCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCATCGCGCCATTg > 1:644937/1‑69 (MQ=255) catcaCCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCATCGCGCCATTg > 1:1923087/1‑69 (MQ=255) catcaCCTTATGTGATGGATCAATTAAAAGAAGCGAAAATCGACCCACTGGATCTGCATCGCGCCATTg > 1:1127733/1‑69 (MQ=255) caCCTTATGTGATGGATCAATTAAAAGAAGCGATAATCGACCCACTGGATCTGCa < 1:2664771/55‑1 (MQ=255) caCCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCg < 1:95979/69‑1 (MQ=255) caCCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCg < 1:1744742/69‑1 (MQ=255) caCCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCg < 1:1914623/69‑1 (MQ=255) caCCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCg < 1:1545549/69‑1 (MQ=255) tATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTg > 1:1416194/1‑69 (MQ=255) tATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTg > 1:2145637/1‑69 (MQ=255) tATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTg > 1:1198086/1‑69 (MQ=255) tATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTg > 1:537640/1‑69 (MQ=255) tATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTg > 1:1508458/1‑69 (MQ=255) tATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTg > 1:1053526/1‑69 (MQ=255) tgtgATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCg < 1:784554/62‑1 (MQ=255) aTGGATCAATTAAAAGACGCGAAAATCGACCCACTGGATCTGCa < 1:858057/44‑1 (MQ=21) aaaaGAGGCGAAAATCGACGAACTGGATTTGCATCGCGTAATTGTCGCATTGAGCGAAAAAATGAAAGc > 1:1330454/1‑69 (MQ=255) aaaaGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGc > 1:3161858/1‑69 (MQ=255) aaaaGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGc > 1:3382077/1‑69 (MQ=255) gagTCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:1230073/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCTAAAAAATGAAAGCGGtt < 1:3257639/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:659153/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:1978313/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:117502/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:254274/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:2161973/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:2468131/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:1673609/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:2294863/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:280324/69‑1 (MQ=255) gagGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGtt < 1:3332300/69‑1 (MQ=255) aaaaTCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGAc < 1:20125/69‑1 (MQ=255) aaaaTCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGAc < 1:2882290/69‑1 (MQ=255) aaaaTCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGAc < 1:2880706/69‑1 (MQ=255) cgacgaACTGGATTTTCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGc < 1:773358/69‑1 (MQ=255) cgacgaACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAg > 1:704717/1‑53 (MQ=255) cgacgaACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACaa > 1:605401/1‑66 (MQ=255) cgacgaACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAAt > 1:1927034/1‑67 (MQ=255) cgacgaACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAAt > 1:1365104/1‑67 (MQ=255) cgacgaACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGc < 1:507093/69‑1 (MQ=255) cgacgaACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGc < 1:2699962/69‑1 (MQ=255) cgacgaACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGc < 1:1448203/69‑1 (MQ=255) cgacgaACTGGATTTGCATCGCGCAATTGTCCCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGc < 1:1622634/69‑1 (MQ=255) ttGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGCCAGTAAGaaaaaa < 1:502435/69‑1 (MQ=255) ttGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGCCAGTAAGaaaaaa < 1:1985011/69‑1 (MQ=255) ttGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGCCAGTAAGaaaaaa < 1:2376253/69‑1 (MQ=255) ttGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGCCAGTAAGaaaaaa < 1:1299721/69‑1 (MQ=255) ttGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGCCAGTAAGaaaaaa < 1:868140/69‑1 (MQ=255) ttGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGCCAGTAAGaaaaaa < 1:936630/69‑1 (MQ=255) | AGTACCTGCGTTCATCACCTTATGTGATGGACCAATTAAAAGAGGCGAAAATCGACGAACTGGATTTGCATCGCGCAATTGTCGCATTGAGCGAAAAAATGAAAGCGGTTGATGACAATGCCAGTAAGAAAAAA > minE/373506‑373639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |