| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 373,918 | T→A | 18.7% | V256V (GTT→GTA) | fepE → | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 373,918 | 0 | T | A | 18.7% | 90.5 / 14.2 | 48 | V256V (GTT→GTA) | fepE | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Reads supporting (aligned to +/- strand): ref base T (19/20); new base A (5/4); total (24/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01 | |||||||||||
GCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTA > minE/373853‑373982 | gCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTa < 1:3214134/68‑1 (MQ=255)gCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTa < 1:1773977/68‑1 (MQ=255) cAAACATTCAGCGCCTCAATTATTCACTTGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTATAc > 1:972565/1‑68 (MQ=255) cAAACATTCAGCGCCTCAATTATTCACTTGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTATAc > 1:813982/1‑68 (MQ=255) cAAACATTCAGCGCCTCAATTATTCACTTGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTATAc > 1:1576180/1‑68 (MQ=255) cAAACATTCAGCGCCTCAATTATTCACTTGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTATAc > 1:1801563/1‑68 (MQ=255) cAAACATTCAGCGCCTCAATTATTCACTTGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTATAc > 1:302781/1‑68 (MQ=255) cAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATg > 1:1324746/1‑67 (MQ=255) cAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATg > 1:274603/1‑67 (MQ=255) tattCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGt > 1:2054102/1‑65 (MQ=255) tattCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGt > 1:1053824/1‑65 (MQ=255) tattCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGt > 1:1205209/1‑65 (MQ=255) cACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGAt < 1:858066/68‑1 (MQ=255) cACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGAt < 1:1915345/68‑1 (MQ=255) cACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGAt < 1:588534/68‑1 (MQ=255) ttgACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTATACAGTAATGGTCAGGCCGTTAAAGATGAc < 1:2323616/66‑1 (MQ=255) ttgACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTATACAGTAATGGTCAGGCCGTTAAAGATGAc < 1:315675/66‑1 (MQ=255) ttgACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTATACAGTAATGGTCAGGCCGTTAAAGATGAc < 1:3363414/66‑1 (MQ=255) ttgACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTATACAGTAATGGTCAGGCCGTTAAAGATGAc < 1:3417613/66‑1 (MQ=255) gCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGt > 1:195092/1‑42 (MQ=255) gCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGt > 1:830492/1‑42 (MQ=255) gCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGAtttt > 1:1905283/1‑69 (MQ=255) gCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGAtttt > 1:772675/1‑69 (MQ=255) gCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGAtttt > 1:1692251/1‑69 (MQ=255) gCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGAtttt > 1:476217/1‑69 (MQ=255) ggCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGAt < 1:1974727/49‑1 (MQ=255) aTTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTaaa > 1:1914911/1‑39 (MQ=255) aTTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTaaa > 1:3099233/1‑39 (MQ=255) aTTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTaaa > 1:3147766/1‑39 (MQ=255) aTTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTaaa > 1:478772/1‑39 (MQ=255) aTTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTaaa > 1:875030/1‑39 (MQ=255) aTTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTaaa > 1:359173/1‑39 (MQ=255) aTTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTaaa > 1:100665/1‑39 (MQ=255) aTTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCgg > 1:935991/1‑68 (MQ=255) gCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACgg < 1:1043761/69‑1 (MQ=255) gCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACgg < 1:837667/69‑1 (MQ=255) cccGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGt < 1:2187079/69‑1 (MQ=255) cccGTTTACAGTAATGGTCAGGCCGTTAAAGAAGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGt < 1:3163521/69‑1 (MQ=255) ccGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTTCAGACGGTa < 1:3166771/69‑1 (MQ=255) ccGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTa < 1:3323474/69‑1 (MQ=255) ccGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTa < 1:2961762/69‑1 (MQ=255) ccGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTa < 1:1726374/69‑1 (MQ=255) ccGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTa < 1:1717251/69‑1 (MQ=255) ccGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTa < 1:171206/69‑1 (MQ=255) ccGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTa < 1:152732/69‑1 (MQ=255) ccGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTa < 1:1238488/69‑1 (MQ=255) ccGTTTACAGTAATGATCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTa < 1:3107018/69‑1 (MQ=255) cGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTa < 1:1157805/68‑1 (MQ=255) | GCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTA > minE/373853‑373982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |