| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 375,663 | G→A | 42.9% | G141G (GGC→GGT) | fepG ← | iron‑enterobactin transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 375,663 | 0 | G | A | 42.9% | 31.7 / 68.8 | 93 | G141G (GGC→GGT) | fepG | iron‑enterobactin transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (19/34); new base A (20/20); total (39/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.74e-01 | |||||||||||
AAAGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGACAATGCCGCCCACCATTGCTGACAGCGC > minE/375603‑375731 | aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc < 1:2863251/62‑1 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc > 1:991386/1‑62 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc < 1:769701/62‑1 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc < 1:690187/62‑1 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc < 1:563097/62‑1 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc > 1:358066/1‑62 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc > 1:2937492/1‑62 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc < 1:2852348/62‑1 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc < 1:2466819/62‑1 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc < 1:2425494/62‑1 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc > 1:2173501/1‑62 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc < 1:1789177/62‑1 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc < 1:1180635/62‑1 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGc < 1:1117613/62‑1 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTgc > 1:620151/1‑68 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTgc > 1:2406200/1‑68 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTgc > 1:3399880/1‑68 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTgc > 1:32287/1‑68 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTgc > 1:3158485/1‑68 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTgc > 1:1081997/1‑68 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTgc > 1:2277591/1‑68 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTgc > 1:935784/1‑68 (MQ=255)aaaGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTgc > 1:1589432/1‑68 (MQ=255) agtaTGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCg > 1:405857/4‑66 (MQ=255) agtaTGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGagc > 1:312338/4‑69 (MQ=255) agtaTGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGagc > 1:3264694/4‑69 (MQ=255) accgataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGt > 1:690673/1‑69 (MQ=255) accgataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGt > 1:3363291/1‑69 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:3237681/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:2817862/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:135071/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:3103346/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:3215709/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:3256599/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:3261527/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:3265346/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:438382/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:587663/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:592019/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:658801/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:858278/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:972746/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:104282/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:2673838/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:1374764/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:1660678/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:2137833/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:2593916/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:23670/69‑1 (MQ=255) gataATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:2397195/69‑1 (MQ=255) cataataATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:889757/68‑1 (MQ=255) cAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGAc < 1:176469/60‑1 (MQ=255) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCa > 1:2833354/1‑39 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:2625750/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:617058/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:1921245/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:206192/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:1237434/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:507496/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:466582/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:1702607/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:425016/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:415283/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:412397/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:1061607/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:34972/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:3284372/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:937244/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:227568/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:2327920/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:2481296/1‑41 (MQ=37) ccGAAAGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGa > 1:120621/1‑41 (MQ=37) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:782365/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:1690977/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:930972/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:2856077/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:1715431/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:678974/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:515284/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:2901859/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:1329392/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:588767/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:1100203/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:2075071/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:114378/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:2207793/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:2243658/69‑1 (MQ=255) aaGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:2419992/69‑1 (MQ=255) aaGGTGTCGATACAGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:390539/69‑1 (MQ=255) aGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:827495/68‑1 (MQ=255) aGGTGTCGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:1628249/68‑1 (MQ=255) gtCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGACAATGCCGCCCACCATTGCt > 1:1007972/1‑67 (MQ=255) cGATACCGTTACGCCAGGCGAGCAGCCAGACCAACAGCGAAGTGACAATGCCGCCCACCAtt < 1:216279/62‑1 (MQ=255) gCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGACAATGCCGCCCACCATTGCTGACAgcgc < 1:1286300/69‑1 (MQ=255) | AAAGGCCACCAGCATGGCGCGAACGCCGATACCGATAATAATCAACCGAAAGGTGTCGATGCCGTTGCGCCAGGCGAGCAGCCAGACCAGCAGCGAAGTGACAATGCCGCCCACCATTGCTGACAGCGC > minE/375603‑375731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |