| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 376,151 | C→T | 37.0% | V312V (GTG→GTA) | fepD ← | iron‑enterobactin transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 376,151 | 0 | C | T | 37.0% | 60.6 / 59.7 | 97 | V312V (GTG→GTA) | fepD | iron‑enterobactin transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (27/34); new base T (18/18); total (45/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
CCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAACAGCAGCAGGGC > minE/376089‑376216 | ccaccTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGa < 1:1171094/68‑1 (MQ=255)ccaccTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGa < 1:2963034/68‑1 (MQ=255)ccaccTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGa < 1:3269666/68‑1 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:107525/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:2302854/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:2139381/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:2586007/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:2817859/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:2066867/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:205239/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:291684/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:2963754/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:1886953/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:3011159/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:3138732/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:3251989/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:1462957/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:1154144/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:3383828/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:3396808/1‑69 (MQ=255) caccTCGCGTTTTACGTCGTACGAGGAAGATCAGCACTGGTGCGCCAATAAAGGCACTGACTACAGaaa > 1:850105/1‑69 (MQ=255) caccTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGaaa > 1:1824301/1‑69 (MQ=255) caccTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGaaa > 1:2950676/1‑69 (MQ=255) caccTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGaaa > 1:263550/1‑69 (MQ=255) aCGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGcaca < 1:99644/69‑1 (MQ=255) aCGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGcaca < 1:3194258/69‑1 (MQ=255) aCGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGcaca < 1:101382/69‑1 (MQ=255) aCGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGcaca < 1:2638086/69‑1 (MQ=255) aCGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGcaca < 1:576916/69‑1 (MQ=255) aCGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGcaca < 1:359163/69‑1 (MQ=255) aCGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGcaca < 1:3341902/69‑1 (MQ=255) aCGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGcaca < 1:3056209/69‑1 (MQ=255) aCGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGcaca < 1:3096427/69‑1 (MQ=255) aCGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGcaca < 1:1788472/69‑1 (MQ=255) aCGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGcaca < 1:2027245/69‑1 (MQ=255) cGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGcaca < 1:1396376/68‑1 (MQ=255) cGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGcaca < 1:2004580/68‑1 (MQ=255) aTCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCAcccgc > 1:1097145/1‑69 (MQ=255) ggTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCgat > 1:669083/1‑65 (MQ=255) ggTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCgat > 1:612932/1‑65 (MQ=255) ggTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCgat > 1:49303/1‑65 (MQ=255) ggTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCgat > 1:1193724/1‑65 (MQ=255) ggTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCgat > 1:1812857/1‑65 (MQ=255) gCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATc < 1:1214874/68‑1 (MQ=255) gCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATc < 1:3211615/68‑1 (MQ=255) gCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATc < 1:3210638/68‑1 (MQ=255) gCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATc < 1:3129121/68‑1 (MQ=255) gCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATc < 1:154715/68‑1 (MQ=255) gCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATc < 1:2349279/68‑1 (MQ=255) gCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATc < 1:486679/68‑1 (MQ=255) gCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATc < 1:315084/68‑1 (MQ=255) gCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATc < 1:1841068/68‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:1301147/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:668173/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:3125295/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:1380971/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:2147403/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:319903/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:1513059/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:892232/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:3048936/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:369620/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:3021503/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:856335/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:383786/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:2613338/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:2899322/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:2871248/69‑1 (MQ=255) aTAAAGGCACTGACTACAGAAACGCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:2695835/69‑1 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCgat < 1:351661/53‑1 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCgat < 1:505765/53‑1 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCgat < 1:2116871/53‑1 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCgat < 1:3283536/53‑1 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCgat < 1:2428502/53‑1 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCgat < 1:3037533/53‑1 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCgat < 1:2125142/53‑1 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAAcagc > 1:1993307/1‑69 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAAcagc > 1:2140337/1‑69 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAAcagc > 1:770802/1‑69 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAAcagc > 1:589780/1‑69 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAAcagc > 1:2068840/1‑69 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAAcagc > 1:2872964/1‑69 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAAcagc > 1:3281181/1‑69 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAAcagc > 1:1288717/1‑69 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAAcagc > 1:2488340/1‑69 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAAcagc > 1:1812190/1‑69 (MQ=255) aaCGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAAcagc > 1:2457333/1‑69 (MQ=255) cTGACTACAGAAACTCGCAGTTCGCCGGGAACAATCACCCGCCCAATGATATCGGCAAAc < 1:212267/60‑1 (MQ=255) tGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAACAGCAgcaggg < 1:1912680/69‑1 (MQ=255) tGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAACAGCAgcaggg < 1:793666/69‑1 (MQ=255) gACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAACAGCAgcagggc > 1:3162614/1‑69 (MQ=255) gACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAACAGCAgcagggc > 1:592438/1‑69 (MQ=255) gACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAACAGCAgcagggc > 1:1343097/1‑69 (MQ=255) gACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAACAGCAgcagggc > 1:3289831/1‑69 (MQ=255) gACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAACAGCAgcagggc > 1:2968442/1‑69 (MQ=255) gACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAACAGCAgcagggc > 1:2399767/1‑69 (MQ=255) gACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAACAGCAgcagggc > 1:2276426/1‑69 (MQ=255) | CCACCTCGCGTTTTACGTCGCACGAGGAAGATCAGCACCGGTGCACCAATAAACGCACTGACCACAGAAACGCGCAGTTCGCCGGGCACAATCACCCGCCCGATGATATCGGCAAACAGCAGCAGGGC > minE/376089‑376216 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |