| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 376,561 | C→T | 19.4% | V176I (GTC→ATC) | fepD ← | iron‑enterobactin transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 376,561 | 0 | C | T | 19.4% | 75.6 / 9.3 | 51 | V176I (GTC→ATC) | fepD | iron‑enterobactin transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (26/15); new base T (6/4); total (32/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01 | |||||||||||
TTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGC > minE/376496‑376623 | ttttAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTc < 1:1207137/69‑1 (MQ=255)ttttAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTc < 1:2899981/69‑1 (MQ=255) aaGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAg < 1:277897/67‑1 (MQ=255) aaGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAg < 1:3297801/67‑1 (MQ=255) aaGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAg < 1:2918977/67‑1 (MQ=255) aaGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAg < 1:2741029/67‑1 (MQ=255) aaGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAg < 1:2682595/67‑1 (MQ=255) aaGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAg < 1:1521047/67‑1 (MQ=255) aaGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAg < 1:1579286/67‑1 (MQ=255) tAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGATGTCAGGATTAAGCAg > 1:1683720/1‑69 (MQ=255) tAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGATGTCAGGATTAAGCAg > 1:1545179/1‑69 (MQ=255) tAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGATGTCAGGATTAAGCAg > 1:252245/1‑69 (MQ=255) tAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGATGTCAGGATTAAGCAg > 1:697542/1‑69 (MQ=255) tAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGATGTCAGGATTAAGCAg > 1:194929/1‑69 (MQ=255) tAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGATGTCAGGATTAAGCAg > 1:1832563/1‑69 (MQ=255) tAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAg < 1:558617/69‑1 (MQ=255) tAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAg < 1:512408/69‑1 (MQ=255) tAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAg < 1:2437500/69‑1 (MQ=255) tAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAg < 1:1566767/69‑1 (MQ=255) ttGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGc > 1:291442/1‑68 (MQ=255) ttGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGc > 1:3408435/1‑68 (MQ=255) ttGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGc > 1:2604807/1‑68 (MQ=255) ttGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGc > 1:1679664/1‑68 (MQ=255) ttGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGc > 1:1994884/1‑68 (MQ=255) ttGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGc > 1:1304291/1‑68 (MQ=255) ttGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGc > 1:28267/1‑68 (MQ=255) tatCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAg < 1:3063126/48‑1 (MQ=255) tatCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGc > 1:3375597/1‑69 (MQ=255) tatCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGc > 1:825431/1‑69 (MQ=255) tatCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGc > 1:2617697/1‑69 (MQ=255) tatCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGc > 1:225576/1‑69 (MQ=255) tCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTg > 1:2870207/1‑69 (MQ=255) ccGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCt < 1:2898874/69‑1 (MQ=255) gCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGt > 1:947342/1‑50 (MQ=255) gCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGt > 1:839425/1‑50 (MQ=255) gCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGt > 1:3302250/1‑50 (MQ=255) gCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGt > 1:2726632/1‑50 (MQ=255) gCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGt > 1:717975/1‑50 (MQ=255) gCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGt > 1:2116292/1‑50 (MQ=255) gCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGt > 1:603600/1‑50 (MQ=255) gCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGt > 1:3014723/1‑50 (MQ=255) cTGATCGTAGATGTCAGGATTAAGCAGGGCGATGCCGCTGGTCGGTCCTTCCAGCACCGCCGCCAgcgc < 1:1016150/69‑1 (MQ=255) cTGATCGTAGATGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAgcgc < 1:2434572/69‑1 (MQ=255) cTGATCGTAGATGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAgcgc < 1:384169/69‑1 (MQ=255) cTGATCGTAGATGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAgcgc < 1:2469683/69‑1 (MQ=255) cGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCAcgc > 1:2237480/1‑69 (MQ=255) cGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCAcgc > 1:616433/1‑69 (MQ=255) cGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCAcgc > 1:2871486/1‑69 (MQ=255) cGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCAcg > 1:1902622/1‑68 (MQ=255) cGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCAcg > 1:3301283/1‑68 (MQ=255) cGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCAAGCACCGCCGCCAGCGCCAcgc > 1:385433/1‑69 (MQ=255) | TTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGC > minE/376496‑376623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |