Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	377,893	G→A	46.1%	G233S (GGT→AGT)	ybdA →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	377,893	0	G	A	46.1%	‑3.3 / 6.2	13	G233S (GGT→AGT)	ybdA	predicted transporter
Reads supporting (aligned to +/- strand):  ref base G (5/2);  new base A (4/2);  total (9/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

GAAATCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCA  >  minE/377826‑377957                                                                    |                                                                 gAAATCATTACTGGCAGTATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTggg                                                                 >  1:2503030/1‑69 (MQ=255) gAAATCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTggg                                                                 >  1:401674/1‑69 (MQ=255)      cATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGAGTGGtt                                                            >  1:1040722/1‑69 (MQ=255)      cATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGAGTGGtt                                                            >  1:1187339/1‑69 (MQ=255)      cATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGAGTGGtt                                                            >  1:3017108/1‑69 (MQ=255)      cATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGAGTGGtt                                                            >  1:549728/1‑69 (MQ=255)       aTTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGttt                                                           >  1:60748/1‑69 (MQ=255)       aTTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGttt                                                           >  1:905613/1‑69 (MQ=255)                                        cccGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGTGTACTGTa                          <  1:2054172/69‑1 (MQ=255)                                          cGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATc                        >  1:1384616/1‑69 (MQ=255)                                          cGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATc                        >  1:2326006/1‑69 (MQ=255)                                                        aTTGCGCTGCTGAGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGAc          <  1:2841053/69‑1 (MQ=255)                                                         ttGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCg                      >  1:2307452/1‑56 (MQ=255)                                                         ttGCGCTGCTGAGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGAc          <  1:2767465/68‑1 (MQ=255)                                                                gctgGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCa  <  1:2504464/69‑1 (MQ=255)                                                                    |                                                                 GAAATCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGGTACTGTATCCGGCGCTGGCTGACAACTGGCA  >  minE/377826‑377957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑