| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 379,015 | T→C | 100% | A131A (GCA→GCG) | fepB ← | iron‑enterobactin transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 379,015 | 0 | T | C | 91.1% | 40.3 / ‑3.4 | 22 | A131A (GCA→GCG) | fepB | iron‑enterobactin transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (0/20); total (2/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATGATTAATGTCGGGGCGATGGTGGAAAGCTGATCATACAGTGCCAGCGCCGAATCCCCGCCGGTTGCGCTAATTAAA > minE/378973‑379051 | gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGGGCCGaa < 1:440993/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:2489371/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:999641/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:694020/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:530657/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:3210911/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:2928798/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:2905141/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:2504460/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:1022621/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:2437048/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:2429759/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:2382075/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:2236073/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:1907443/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:187087/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:1503834/55‑1 (MQ=255)gatgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:1412052/55‑1 (MQ=255) atgatTAATGTTGGGGCGATGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:956758/54‑1 (MQ=37) ggggCGATGGTGGAAAGCTGATCATACAGTGCCAGCGCCGAATCCCCGCCGGTTGCGCTAATTaaa > 1:2936403/1‑66 (MQ=255) ggggCGATGGTGGAAAGCTGATCATACAGTGCCAGCGCCGAATCCCCGCCGGTTGCGCTAATTaaa > 1:1669027/1‑66 (MQ=255) aTGGTAGAAAGCTGATCGTACAGCGCCAGCGCCGaa < 1:1345660/36‑1 (MQ=21) | GATGATTAATGTCGGGGCGATGGTGGAAAGCTGATCATACAGTGCCAGCGCCGAATCCCCGCCGGTTGCGCTAATTAAA > minE/378973‑379051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |