Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	380,351	C→T	54.2%	V190V (GTC→GTT)	entC →	isochorismate synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	380,351	0	C	T	54.2%	2.2 / 38.3	48	V190V (GTC→GTT)	entC	isochorismate synthase 1
Reads supporting (aligned to +/- strand):  ref base C (13/9);  new base T (16/10);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GGAACGGTTGATTGCGCAAAACCCGGTTAGTTACAACTTCCATGTTCCGCTGGCTGATGGTGGCGTCCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGTTTTAGCTCCATTCC  >  minE/380285‑380413                                                                   |                                                               ggAACGGTTGATTGCGCAAAACCCGGTTAGTTACAACTTCCATGTTCCGCTGGCTGATGGTGGCGTCct                                                              >  1:775522/1‑69 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:2294182/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:3017357/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:2952684/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:308700/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:3316456/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:2694179/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:2690356/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:2654608/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:373662/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:2544565/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:2391792/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:1360165/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:1609150/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:809736/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAActgct                                      >  1:19977/1‑68 (MQ=255)                          gttagttaCAACTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCACCCCGGaa                                           >  1:2346145/1‑63 (MQ=255)                           ttagttaCAACTTCCATGTTCCGCTGGCTGATGGTGGCGTCCTGCTGGGGGCCAGCCCGGAACtgctgc                                    <  1:1799698/69‑1 (MQ=255)                                     cTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGa                          <  1:2598085/69‑1 (MQ=255)                                     cTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGa                          <  1:863971/69‑1 (MQ=255)                                     cTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGa                          <  1:1837581/69‑1 (MQ=255)                                     cTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGa                          <  1:1925579/69‑1 (MQ=255)                                     cTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGa                          <  1:1928352/69‑1 (MQ=255)                                     cTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGa                          <  1:1962026/69‑1 (MQ=255)                                     cTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGa                          <  1:2451748/69‑1 (MQ=255)                                     cTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGa                          <  1:489950/69‑1 (MQ=255)                                     cTTCCATGTTCCGTTGGCTGATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGa                          <  1:2129327/69‑1 (MQ=255)                                            gTTCCGCTGGCTGATGGTGGCGTCCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCTAg                   <  1:696172/69‑1 (MQ=255)                                            gTTCCGCTGGCTGATGGTGGCGTCCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAg                   <  1:827103/69‑1 (MQ=255)                                            gTTCCGCTGGCTGATGGTGGCGTCCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAg                   <  1:928779/69‑1 (MQ=255)                                            gTTCCGCTGGCTGATGGTGGCGTCCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAg                   <  1:2899052/69‑1 (MQ=255)                                            gTTCCGCTGGCTGATGGTGGCGTCCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAg                   <  1:1966478/69‑1 (MQ=255)                                            gTTCCGCTGGCTGATGGTGGCGTCCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAg                   <  1:2282236/69‑1 (MQ=255)                                             ttCCGCTGGCTGATGGTGGCGTCCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAg                   <  1:1727306/68‑1 (MQ=255)                                                  ctggctgATGGTGGCGTCCTGCTGGGGGCCAGCCCGGAACtgctgc                                    <  1:90223/46‑1 (MQ=255)                                                   tggctgATGGTGGCGTTCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGa                          <  1:2413371/55‑1 (MQ=255)                                                            ttggCGTCCTGTTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGtttt             >  1:52650/2‑59 (MQ=255)                                                            gtggCGTCCTGTTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGtttt             >  1:1348089/1‑59 (MQ=255)                                                            gtggCGTCCTGTTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGtttt             >  1:1351692/1‑59 (MQ=255)                                                            gtggCGTCCTGTTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGtttt             >  1:2019442/1‑59 (MQ=255)                                                            gtggCGTCCTGTTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGtttt             >  1:626571/1‑59 (MQ=255)                                                            gtggCGTCCTGTTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGtttt             >  1:44064/1‑59 (MQ=255)                                                            gtggCGTCCTGTTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGtttt             >  1:2629315/1‑59 (MQ=255)                                                            gtggCGTCCTGTTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGtttt             >  1:3171882/1‑59 (MQ=255)                                                            gtggCGTCCTGTTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGtttt             >  1:3106476/1‑59 (MQ=255)                                                            gtggCGTCCTGTTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGtttt             >  1:2922216/1‑59 (MQ=255)                                                            gtggCGACCTGTTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGtttt             >  1:4115/1‑59 (MQ=255)                                                             tggCGTCCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGTTTTAGCTCCATTcc  >  1:299616/1‑69 (MQ=255)                                                                   |                                                               GGAACGGTTGATTGCGCAAAACCCGGTTAGTTACAACTTCCATGTTCCGCTGGCTGATGGTGGCGTCCTGCTGGGGGCCAGCCCGGAACTGCTGCTACGTAAAGACGGCGAGCGTTTTAGCTCCATTCC  >  minE/380285‑380413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑