| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 382,496 | G→A | 18.7% | V510V (GTG→GTA) | entE → | 2,3‑dihydroxybenzoate‑AMP ligase component of enterobactin synthase multienzyme complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 382,496 | 0 | G | A | 18.7% | 114.2 / 15.3 | 74 | V510V (GTG→GTA) | entE | 2,3‑dihydroxybenzoate‑AMP ligase component of enterobactin synthase multienzyme complex |
| Reads supporting (aligned to +/- strand): ref base G (31/29); new base A (12/2); total (43/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.29e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCAC > minE/382432‑382563 | tGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGAtt > 1:3419727/1‑69 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:582096/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:1828531/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:205592/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:1686224/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:1650801/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:2376704/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:687515/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:2846813/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:1530997/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:1360199/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:3030573/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:568573/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:515194/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:3351184/68‑1 (MQ=255) tGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTg < 1:3395660/68‑1 (MQ=255) gCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGAc > 1:757221/1‑69 (MQ=255) gCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGAc > 1:948721/1‑69 (MQ=255) ggTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCgg > 1:359081/1‑68 (MQ=255) ggTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCgg > 1:253218/1‑68 (MQ=255) ggTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCgg > 1:1567294/1‑68 (MQ=255) ggTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCgg > 1:1571604/1‑68 (MQ=255) ggTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCgg > 1:943995/1‑68 (MQ=255) ggTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCgg > 1:1349152/1‑68 (MQ=255) ggTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCgg > 1:1608253/1‑68 (MQ=255) gCCGATTTTAAATTACCGGATCGCGTGGAGTGTCTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTc > 1:1193745/1‑69 (MQ=255) gCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGt > 1:2647285/1‑68 (MQ=255) gCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTc > 1:819056/1‑69 (MQ=255) gCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTc > 1:2810980/1‑69 (MQ=255) gCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTc > 1:2806247/1‑69 (MQ=255) gCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTc > 1:1790820/1‑69 (MQ=255) gCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTc > 1:1335758/1‑69 (MQ=255) gCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTc > 1:2527664/1‑69 (MQ=255) gCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTc > 1:73108/1‑69 (MQ=255) gCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTc > 1:1065807/1‑69 (MQ=255) gCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTc > 1:1913449/1‑69 (MQ=255) gCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCAATTCCGCTGACGGCGGTCGGGAAAGTc > 1:1151655/1‑69 (MQ=255) cGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCg > 1:617563/1‑68 (MQ=255) gAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGa > 1:248888/1‑68 (MQ=255) gAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGa > 1:3310136/1‑68 (MQ=255) tAAATTACCGGATCTCGTGGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAg > 1:2177537/1‑59 (MQ=255) tAAATTACCGGATCGCGTGGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAg > 1:2734532/1‑59 (MQ=255) tAAATTACCGGATCGCGTGGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAg > 1:3207518/1‑59 (MQ=255) tAAATTACCGGATCGCGTGGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAg > 1:429025/1‑59 (MQ=255) tAAATTACCGGATCGCGTGGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAg > 1:3220404/1‑59 (MQ=255) tAAATTACCGGATCGCGTGGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAg > 1:3022501/1‑59 (MQ=255) tAAATTACCGGATCGCGTGGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAg > 1:2965484/1‑59 (MQ=255) tAAATTACCGGATCGCGTGGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAg > 1:2791185/1‑59 (MQ=255) tAAATTACCGGATCGCGTGGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAg > 1:1011928/1‑59 (MQ=255) tAAATTACCGGATCGCGTGGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAg > 1:2548771/1‑59 (MQ=255) tAAATTACCGGATCGCGTGGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAg > 1:2200580/1‑59 (MQ=255) tAAATTACCGGATCGCGTGGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAg > 1:2113715/1‑59 (MQ=255) aTTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAAc < 1:1641925/68‑1 (MQ=255) ttACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACa < 1:2567209/68‑1 (MQ=255) tACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACTGCGGTCGGGAAAGTCGATAAAAAACAAt < 1:168651/69‑1 (MQ=255) tACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAt < 1:671609/69‑1 (MQ=255) tACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAt < 1:726656/69‑1 (MQ=255) tACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAt < 1:754226/69‑1 (MQ=255) tACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAt < 1:2706824/69‑1 (MQ=255) tACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAt < 1:1165653/69‑1 (MQ=255) tACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAt < 1:2947011/69‑1 (MQ=255) tACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAt < 1:1596863/69‑1 (MQ=255) tACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAt < 1:2493750/69‑1 (MQ=255) tACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAt < 1:2409270/69‑1 (MQ=255) tACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGGCGATAAAAAACAAt < 1:1020045/69‑1 (MQ=255) aCCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAtt > 1:386715/1‑69 (MQ=255) aCCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAtt > 1:3210204/1‑69 (MQ=255) aCCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAtt > 1:636424/1‑69 (MQ=255) aCCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAtt > 1:640427/1‑69 (MQ=255) aCCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAtt > 1:2349083/1‑69 (MQ=255) aCCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAAtt > 1:2024621/1‑69 (MQ=255) aTCGCTTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTc < 1:309946/69‑1 (MQ=255) ggAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGtggct < 1:409274/69‑1 (MQ=255) ggAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGtggct < 1:1759090/69‑1 (MQ=255) gtgtgGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGt < 1:2952128/69‑1 (MQ=255) tgGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCAc > 1:2618941/1‑69 (MQ=255) tgGATTAACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCAc > 1:3052915/1‑69 (MQ=255) | TGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCAC > minE/382432‑382563 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |