Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	43,712	T→C	24.6%	I76I (ATT→ATC)	yaaU →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	43,712	0	T	C	24.6%	101.9 / 29.2	85	I76I (ATT→ATC)	yaaU	predicted transporter
Reads supporting (aligned to +/- strand):  ref base T (44/20);  new base C (9/12);  total (53/32)
Fisher's exact test for biased strand distribution p-value = 4.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTG  >  minE/43658‑43778                                                       |                                                                   gggCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCg                                                       <  1:419611/68‑1 (MQ=255) gggCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCg                                                       <  1:3340088/68‑1 (MQ=255) gggCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCg                                                       <  1:248031/68‑1 (MQ=255) gggCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCg                                                       <  1:837519/68‑1 (MQ=255) gggCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCg                                                       <  1:1956391/68‑1 (MQ=255) gggCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCg                                                       <  1:862948/68‑1 (MQ=255)  ggCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGa                                                     >  1:941102/1‑69 (MQ=255)  ggCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGa                                                     >  1:2649551/1‑69 (MQ=255)         gAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGgcgc                                               >  1:2152314/1‑68 (MQ=255)         gAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGgcgc                                               >  1:521121/1‑68 (MQ=255)         gAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGgcgc                                               >  1:3216126/1‑68 (MQ=255)         gAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGgcgc                                               >  1:3037991/1‑68 (MQ=255)         gAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGgcgc                                               >  1:2939592/1‑68 (MQ=255)         gAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGgcgc                                               >  1:3142970/1‑68 (MQ=255)         gAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGgcgc                                               >  1:2340525/1‑68 (MQ=255)         gAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGgcgc                                               >  1:1909849/1‑68 (MQ=255)         gAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGgcg                                                >  1:2221137/1‑67 (MQ=255)              cTCGCCGGGCTGTTCGTTGTCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATg                                         <  1:406901/69‑1 (MQ=255)              cTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAAt                                          >  1:2373158/1‑68 (MQ=255)              cTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAAt                                          >  1:334212/1‑68 (MQ=255)              cTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATg                                         <  1:186004/69‑1 (MQ=255)              cTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATg                                         >  1:699910/1‑69 (MQ=255)              cTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATg                                         <  1:1911336/69‑1 (MQ=255)              cTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATg                                         <  1:3296941/69‑1 (MQ=255)              cTCGCCGGGCTGGTCGTTGGCACATCGCTGTTTGGTTATATTTCCGAt                                                              >  1:398391/1‑48 (MQ=255)                   cGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTc                                      <  1:2721220/67‑1 (MQ=255)                   cGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTc                                      <  1:1003306/67‑1 (MQ=255)                   cGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTc                                      <  1:2598187/67‑1 (MQ=255)                   cGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTc                                      <  1:696335/67‑1 (MQ=255)                   cGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTc                                      <  1:424723/67‑1 (MQ=255)                   cGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTc                                      <  1:3315357/67‑1 (MQ=255)                    gggCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTcc                                     >  1:1888890/1‑67 (MQ=255)                    gggCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTc                                   >  1:1747473/1‑69 (MQ=255)                    gggCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTc                                   >  1:883206/1‑69 (MQ=255)                        tGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCAtt                                >  1:527871/1‑68 (MQ=255)                        tGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCAtt                                >  1:3166961/1‑68 (MQ=255)                        tGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCAtt                                >  1:2767262/1‑68 (MQ=255)                        tGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCAtt                                >  1:591546/1‑68 (MQ=255)                        tGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCAtt                                >  1:645248/1‑68 (MQ=255)                        tGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCAtt                                >  1:881883/1‑68 (MQ=255)                        tGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCAtt                                >  1:1682947/1‑68 (MQ=255)                         gTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGa                              <  1:1256713/69‑1 (MQ=255)                         gTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGa                              <  1:3320762/69‑1 (MQ=255)                            cGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGa                                                     >  1:537110/1‑43 (MQ=255)                            cGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:1198172/1‑60 (MQ=255)                            cGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:3053791/1‑60 (MQ=255)                            cGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:1105903/1‑60 (MQ=255)                            cGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:533164/1‑60 (MQ=255)                            cGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:18673/1‑60 (MQ=255)                            cGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:183658/1‑60 (MQ=255)                            cGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:856275/1‑60 (MQ=255)                            cGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:1370031/1‑60 (MQ=255)                            cGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:1551552/1‑60 (MQ=255)                            cGTTCGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:458697/1‑60 (MQ=255)                                   acaTCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGcc                    >  1:2624601/1‑69 (MQ=255)                                     aTCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGata                            <  1:3427334/59‑1 (MQ=255)                                     aTCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGata                            <  1:140598/59‑1 (MQ=255)                                     aTCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGata                            <  1:3418541/59‑1 (MQ=255)                                     aTCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGata                            <  1:3269807/59‑1 (MQ=255)                                     aTCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGata                            <  1:2962365/59‑1 (MQ=255)                                     aTCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGata                            <  1:1167698/59‑1 (MQ=255)                                     aTCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGata                            <  1:2421739/59‑1 (MQ=255)                                     aTCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGata                            <  1:2186188/59‑1 (MQ=255)                                     aTCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGata                            <  1:1695983/59‑1 (MQ=255)                                     aTCGCTGTTTGGCTATATCTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGata                            <  1:1566720/59‑1 (MQ=255)                                     aTCGCTGTTTGGCTATATCTACGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGata                            <  1:2342880/59‑1 (MQ=255)                                        gCTGTTTGGTTATATTTCCGATAAAGTCGGACGgcgc                                               >  1:3204899/1‑37 (MQ=255)                                        gCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:2892922/1‑48 (MQ=255)                                        gCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:3434392/1‑48 (MQ=255)                                        gCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:315131/1‑48 (MQ=255)                                        gCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:3150911/1‑48 (MQ=255)                                        gCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:2200501/1‑48 (MQ=255)                                        gCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCgg               <  1:1587041/69‑1 (MQ=255)                                        gCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCgg               <  1:2741433/69‑1 (MQ=255)                                        gCTGTTAGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCt                                    >  1:442401/1‑48 (MQ=255)                                         gtGTTTGGCTATATCTCCGATAAAGTCGGACGGCGCATAATGTTCCTCATTGata                            <  1:1052513/54‑1 (MQ=255)                                              tGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGat         >  1:1047544/1‑69 (MQ=255)                                              tGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGat         >  1:1405806/1‑69 (MQ=255)                                              tGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGat         >  1:2082943/1‑69 (MQ=255)                                              tGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGat         >  1:2135183/1‑69 (MQ=255)                                              tGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGat         >  1:324526/1‑69 (MQ=255)                                                   atatTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCgg    >  1:2421452/1‑69 (MQ=255)                                                   atatTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCgg    >  1:1639578/1‑69 (MQ=255)                                                   atatTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCgg    >  1:46732/1‑69 (MQ=255)                                                   atatTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCgg    >  1:390105/1‑69 (MQ=255)                                                      tttCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTg  >  1:796373/1‑68 (MQ=255)                                                      tttCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTg  >  1:2874452/1‑68 (MQ=255)                                                       |                                                                   GGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTG  >  minE/43658‑43778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑