| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 389,181 | A→G | 35.0% | S233S (TCA→TCG) | ybdL → | methionine aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 389,181 | 0 | A | G | 35.0% | 37.1 / 33.5 | 60 | S233S (TCA→TCG) | ybdL | methionine aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (27/12); new base G (7/14); total (34/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
CAGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCC > minE/389119‑389241 | caGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGc < 1:3085969/69‑1 (MQ=255)caGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGc < 1:225380/69‑1 (MQ=255) gCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGa > 1:2313353/1‑69 (MQ=255) cATGCCAGTGTTCTGGCGCATCCGCAGCTGCGTGAGCGGTCAGTGGCGGTTTCTTCATTTGGCAAGAcc < 1:1129021/69‑1 (MQ=255) cATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGAcc < 1:3338323/69‑1 (MQ=255) cATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGAcc < 1:338402/69‑1 (MQ=255) aTGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGAcc < 1:2296395/68‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTGAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:1439648/69‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTGAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:3175842/69‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTGAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:3098355/69‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTGAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:1265173/69‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTGAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:2823280/69‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTGAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:391184/69‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTGAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:2330389/69‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTGAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:605961/69‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTGAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:1890535/69‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTGAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:1756766/69‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTGAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:325763/69‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTGAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:682184/69‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTGAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:1500850/69‑1 (MQ=255) gtgCTGGCGCATCCGCAATTGCGTCAGCGAGCGGTGGCGGTGTCATCGTTTGGCAAGACCTATCATATg < 1:1932213/69‑1 (MQ=255) tgCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCAtt > 1:2859832/1‑49 (MQ=255) tgCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGAcc < 1:3340606/59‑1 (MQ=255) tgCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGAcc < 1:1471997/59‑1 (MQ=255) tgCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCat > 1:967852/1‑65 (MQ=255) tgCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCat < 1:1509782/65‑1 (MQ=255) tgCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCat > 1:2703159/1‑65 (MQ=255) tgCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGa > 1:137912/1‑69 (MQ=255) tgCTGGCGCATCCGCAGCTGCGTGAGCCGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCat > 1:488446/1‑65 (MQ=255) gCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTgg > 1:1607110/1‑68 (MQ=255) gCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTgg > 1:1847565/1‑68 (MQ=255) gCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTgg > 1:1276176/1‑68 (MQ=255) gCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTgg > 1:420974/1‑68 (MQ=255) gCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTgg > 1:615155/1‑68 (MQ=255) aGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATtgtg > 1:2753837/1‑69 (MQ=255) ggCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTgc < 1:1114604/69‑1 (MQ=255) ggCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTgc < 1:3221641/69‑1 (MQ=255) gCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTgc < 1:3430252/68‑1 (MQ=255) gCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAgcg > 1:1339321/1‑69 (MQ=255) ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTgg > 1:3010036/1‑47 (MQ=255) ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:799519/1‑69 (MQ=255) ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:2809399/1‑69 (MQ=255) ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:3142824/1‑69 (MQ=255) ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:1683570/1‑69 (MQ=255) ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:1982728/1‑69 (MQ=255) ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:3303420/1‑69 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:2011179/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:97426/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:971379/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:1491399/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:48634/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:210468/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:3388225/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:3259853/1‑68 (MQ=255) ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:516525/1‑67 (MQ=255) ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:2547129/1‑67 (MQ=255) ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:2637388/1‑67 (MQ=255) ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:1470549/1‑67 (MQ=255) ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:1386122/1‑67 (MQ=255) ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:282809/1‑67 (MQ=255) | CAGGGCCATGCCAGTGTGCTGGCGCATCCGCAGCTGCGTGAGCGGGCAGTGGCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCC > minE/389119‑389241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |