| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 389,235 | A→T | 37.0% | P251P (CCA→CCT) | ybdL → | methionine aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 389,235 | 0 | A | T | 37.0% | 68.7 / 74.6 | 89 | P251P (CCA→CCT) | ybdL | methionine aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (28/28); new base T (3/30); total (31/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.22e-01 | |||||||||||
GCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCAC > minE/389170‑389302 | gCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAgcg > 1:1339321/1‑69 (MQ=255) ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:3142824/1‑69 (MQ=255) ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:3303420/1‑69 (MQ=255) ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:1982728/1‑69 (MQ=255) ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:799519/1‑69 (MQ=255) ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:1683570/1‑69 (MQ=255) ggTGTCATCGTTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTtgcgcctgcgcc > 1:2809399/1‑69 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:48634/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:1491399/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:3259853/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:3388225/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:210468/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:2011179/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:971379/1‑68 (MQ=255) tttCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:97426/1‑68 (MQ=255) ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:1386122/1‑67 (MQ=255) ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:2637388/1‑67 (MQ=255) ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:282809/1‑67 (MQ=255) ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:1470549/1‑67 (MQ=255) ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:2547129/1‑67 (MQ=255) ttcttcATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGccc > 1:516525/1‑67 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2994785/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2660547/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1053493/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2512579/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2420899/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2826495/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2400174/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2398030/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:284910/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2880696/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:289473/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2954733/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2520722/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:3063319/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:3402217/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:795639/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:827283/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:956851/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:988601/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1070505/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1331200/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1375073/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1246128/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2006572/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1118673/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2224989/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2238681/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:2319101/68‑2 (MQ=255) gCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1082285/68‑2 (MQ=255) cAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGag < 1:1198923/67‑2 (MQ=255) tCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAgg > 1:687168/1‑68 (MQ=255) atatGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTa > 1:2093868/1‑68 (MQ=255) atatGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTAc > 1:2280349/1‑69 (MQ=255) atatGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTAc > 1:3281154/1‑69 (MQ=255) atatGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTAc > 1:2185521/1‑69 (MQ=255) tGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATc > 1:1456229/1‑69 (MQ=255) gACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGAGATCCGCAAAGTGCATc > 1:1828158/1‑68 (MQ=255) gACCGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCGCCCATCAGCGCCGAGATCCGCAAAGTGCATc > 1:1642729/1‑68 (MQ=255) gACAGGCTGGAAAGTGGGTTATTGCGTTGCGCCTGCG‑CCATCAGCGCCGAGATCCGCAAAGTGCATCAg > 1:490343/1‑69 (MQ=255) cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:122172/69‑1 (MQ=255) cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:2611535/69‑1 (MQ=255) cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:777118/69‑1 (MQ=255) cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:1192918/69‑1 (MQ=255) cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:1307378/69‑1 (MQ=255) cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:3372185/69‑1 (MQ=255) cGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:2461463/69‑1 (MQ=255) ggCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTa < 1:1420467/68‑1 (MQ=255) tGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCtttt > 1:2311586/1‑69 (MQ=255) tGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCtttt > 1:1386311/1‑69 (MQ=255) ggTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTc > 1:987397/1‑40 (MQ=255) ggTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCg > 1:971663/1‑69 (MQ=255) tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:1285544/69‑1 (MQ=255) tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:527047/69‑1 (MQ=255) tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:783210/69‑1 (MQ=255) tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:3360409/69‑1 (MQ=255) tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:3125334/69‑1 (MQ=255) tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:914414/69‑1 (MQ=255) tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:1624180/69‑1 (MQ=255) tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:92415/69‑1 (MQ=255) tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:2041407/69‑1 (MQ=255) tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:2623186/69‑1 (MQ=255) tATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTAGCAAGGTACATCAGTATCTGACCTTTTCGGTg < 1:1177302/69‑1 (MQ=255) tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGaa < 1:2343094/68‑1 (MQ=255) tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGaa < 1:2847338/68‑1 (MQ=255) tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGaa < 1:2846773/68‑1 (MQ=255) tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGaa < 1:2403468/68‑1 (MQ=255) tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAAt > 1:706978/1‑69 (MQ=255) tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAAt > 1:3148235/1‑69 (MQ=255) tgtgTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAAt > 1:841760/1‑69 (MQ=255) gcCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCgg < 1:1528674/68‑1 (MQ=255) gcCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCgg < 1:2162635/68‑1 (MQ=255) ccAGCGCCCATCAGCGCC‑AAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGcac < 1:2387007/69‑1 (MQ=255) cAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGcac < 1:2287666/69‑1 (MQ=255) cAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGcac < 1:2434807/69‑1 (MQ=255) aGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGcac < 1:3193055/68‑1 (MQ=255) | GCGGTTTCTTCATTTGGCAAGACCTATCATATGACCGGCTGGAAAGTGGGTTATTGTGTTGCGCCAGCGCCCATCAGCGCCGAAATTCGCAAGGTACATCAGTATCTGACCTTTTCGGTGAATACCCCGGCAC > minE/389170‑389302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |