| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 390,449 | T→C | 50.0% | I340V (ATC→GTC) | ybdN ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 390,449 | 0 | T | C | 50.0% | ‑3.7 / 40.6 | 48 | I340V (ATC→GTC) | ybdN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (13/11); new base C (20/4); total (33/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCAGGAGGGGATATCTTTCGCGCCAATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTGT > minE/390392‑390509 | gccAGGAGGGGATATCTTTCGCGCCAATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCt < 1:1252792/69‑1 (MQ=255) ccAGGAGGGGATATCTTTCGCGCCAATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCt < 1:2918930/68‑1 (MQ=255) gaggGGATATCTTTCGCGCCAATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATAc < 1:324138/69‑1 (MQ=255) gaggGGATATCTTTCGCGCCAATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATAc < 1:1762349/69‑1 (MQ=255) gaggGGATATCTTTCGCGCCAATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATAc < 1:572102/69‑1 (MQ=255) gATATCTTTCGCGCCAATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAg > 1:3335068/1‑67 (MQ=255) gATATCTTTCGCGCCAATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGt > 1:2105034/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATAc > 1:565700/1‑48 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGcaa > 1:1546246/1‑65 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGcaa > 1:2663050/1‑65 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGcaa > 1:493522/1‑65 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:76408/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:1255411/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:3263806/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:1213377/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:601329/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:1860803/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:234022/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:2542416/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:2776372/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:2581629/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:2463874/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:2355805/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:3087890/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:1547293/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:1416713/1‑68 (MQ=255) aTGTCCCAGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:2627298/1‑69 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:3037099/69‑1 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:2878199/69‑1 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:1163178/69‑1 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:271046/69‑1 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:1757446/69‑1 (MQ=255) tCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTa > 1:2839368/1‑69 (MQ=255) tCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTa > 1:175856/1‑69 (MQ=255) gCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGt > 1:2410886/1‑69 (MQ=255) gCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGt > 1:1809174/1‑69 (MQ=255) tGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGt < 1:1804006/67‑1 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:625339/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:638408/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:940696/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:2647228/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:1777180/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:1348518/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTCCAAATAAATGGCGAtttt > 1:1196759/1‑53 (MQ=255) aCCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAATTTTGTTGCGGTAATGCTCAGCGGt < 1:3221653/68‑1 (MQ=255) aCCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAATTTTGTTGCGGTAATGCTCAGCGGt < 1:3148511/68‑1 (MQ=255) aCCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAATTTTGTTGCGGTAATGCTCAGCGGt < 1:2727004/68‑1 (MQ=255) aCCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAATTTTGTTGCGGTAATGCTCAGCGGt < 1:1541861/68‑1 (MQ=255) | GCCAGGAGGGGATATCTTTCGCGCCAATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTGT > minE/390392‑390509 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |