| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 390,468 | G→A | 37.6% | H333H (CAC→CAT) | ybdN ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 390,468 | 0 | G | A | 37.6% | 35.1 / 35.3 | 61 | H333H (CAC→CAT) | ybdN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (29/9); new base A (19/4); total (48/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATATCTTTCGCGCCAATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCA > minE/390402‑390532 | gATATCTTTCGCGCCAATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAg > 1:3335068/1‑67 (MQ=255)gATATCTTTCGCGCCAATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGt > 1:2105034/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGcaa > 1:493522/1‑65 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGcaa > 1:2663050/1‑65 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGcaa > 1:1546246/1‑65 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:76408/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:234022/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:2542416/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:1860803/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:3263806/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:1255411/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:1213377/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:601329/1‑69 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:1547293/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:3087890/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:2776372/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:1416713/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:2581629/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:2463874/1‑68 (MQ=255) aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:2355805/1‑68 (MQ=255) aTGTCCCAGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:2627298/1‑69 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:3037099/69‑1 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:2878199/69‑1 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:271046/69‑1 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:1163178/69‑1 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:1757446/69‑1 (MQ=255) tCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTa > 1:2839368/1‑69 (MQ=255) tCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTa > 1:175856/1‑69 (MQ=255) gCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGt > 1:2410886/1‑69 (MQ=255) gCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGt > 1:1809174/1‑69 (MQ=255) tGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGt < 1:1804006/67‑1 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:940696/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:1348518/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:1777180/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:2647228/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:625339/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:638408/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTCCAAATAAATGGCGAtttt > 1:1196759/1‑53 (MQ=255) aCCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAATTTTGTTGCGGTAATGCTCAGCGG‑t < 1:2727004/68‑1 (MQ=255) aCCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAATTTTGTTGCGGTAATGCTCAGCGG‑t < 1:1541861/68‑1 (MQ=255) aCCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAATTTTGTTGCGGTAATGCTCAGCGG‑t < 1:3221653/68‑1 (MQ=255) aCCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAATTTTGTTGCGGTAATGCTCAGCGG‑t < 1:3148511/68‑1 (MQ=255) gCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt > 1:528662/1‑38 (MQ=255) gCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt > 1:665918/1‑38 (MQ=255) gCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt > 1:173406/1‑38 (MQ=255) gCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt > 1:467081/1‑38 (MQ=255) gCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt > 1:2284691/1‑38 (MQ=255) ttCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGc < 1:2921800/67‑1 (MQ=255) ttCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGc < 1:2577741/67‑1 (MQ=255) tCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGc < 1:1007592/66‑1 (MQ=255) aTACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTGTTTTTTTCCGGCATGCTATTg > 1:2617503/1‑68 (MQ=255) aTACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTg > 1:1496973/1‑67 (MQ=255) aTACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTg > 1:186598/1‑67 (MQ=255) acaaGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:2784897/4‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:1680580/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:357313/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:1922719/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:1995700/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:846548/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:2181756/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCAGGCTATTGagc > 1:3076934/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGT‑ATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagca > 1:401556/1‑68 (MQ=255) | GATATCTTTCGCGCCAATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCA > minE/390402‑390532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |