| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 390,483 | G→A | 21.8% | I328I (ATC→ATT) | ybdN ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 390,483 | 0 | G | A | 21.8% | 127.0 / 25.7 | 91 | I328I (ATC→ATT) | ybdN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (38/33); new base A (16/4); total (54/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
ATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCATATT > minE/390418‑390547 | aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:234022/1‑69 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:76408/1‑69 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:1213377/1‑69 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:1255411/1‑69 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:601329/1‑69 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:2542416/1‑69 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:3263806/1‑69 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:1860803/1‑69 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:2776372/1‑68 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:2355805/1‑68 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:2463874/1‑68 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:2581629/1‑68 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:1547293/1‑68 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:1416713/1‑68 (MQ=255)aTGTCCCCGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAt > 1:3087890/1‑68 (MQ=255)aTGTCCCAGTGCTGGGTCTGTGGCACCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAAtt > 1:2627298/1‑69 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:2878199/69‑1 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:1163178/69‑1 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:3037099/69‑1 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:1757446/69‑1 (MQ=255) tGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt < 1:271046/69‑1 (MQ=255) tCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTa > 1:175856/1‑69 (MQ=255) tCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTa > 1:2839368/1‑69 (MQ=255) gCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGt > 1:1809174/1‑69 (MQ=255) gCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGt > 1:2410886/1‑69 (MQ=255) tGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGt < 1:1804006/67‑1 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:2647228/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:940696/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:625339/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:638408/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:1777180/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAtttt > 1:1348518/1‑53 (MQ=255) tgtgGAACCTCGATGCCTTTTTTCTGATACCAGTCCAAATAAATGGCGAtttt > 1:1196759/1‑53 (MQ=255) aCCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAATTTTGTTGCGGTAATGCTCAGCGG‑t < 1:2727004/68‑1 (MQ=255) aCCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAATTTTGTTGCGGTAATGCTCAGCGG‑t < 1:1541861/68‑1 (MQ=255) aCCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAATTTTGTTGCGGTAATGCTCAGCGG‑t < 1:3148511/68‑1 (MQ=255) aCCTCGACACCTTTTTTCTGATACCAATGCAAGTAAATGGCAATTTTGTTGCGGTAATGCTCAGCGG‑t < 1:3221653/68‑1 (MQ=255) gCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt > 1:173406/1‑38 (MQ=255) gCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt > 1:467081/1‑38 (MQ=255) gCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt > 1:528662/1‑38 (MQ=255) gCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt > 1:665918/1‑38 (MQ=255) gCCTTTTTTCTGATACCAGTGCAAATAAATGGCGAttt > 1:2284691/1‑38 (MQ=255) ttCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGc < 1:2577741/67‑1 (MQ=255) ttCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGc < 1:2921800/67‑1 (MQ=255) tCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGc < 1:1007592/66‑1 (MQ=255) aTACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTGTTTTTTTCCGGCATGCTATTg > 1:2617503/1‑68 (MQ=255) aTACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTg > 1:1496973/1‑67 (MQ=255) aTACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTg > 1:186598/1‑67 (MQ=255) acaaGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:2784897/4‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:357313/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:846548/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:1680580/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:2181756/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:1995700/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagc > 1:1922719/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCAGGCTATTGagc > 1:3076934/1‑68 (MQ=255) aCCAGTGCAAATAAATGGCGATTTTATTGCGGT‑ATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGagca > 1:401556/1‑68 (MQ=255) cAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCaataa < 1:3381976/69‑1 (MQ=255) cAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCaataa < 1:2174694/69‑1 (MQ=255) cAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCaataa > 1:2101167/1‑69 (MQ=255) cAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCaataa > 1:2047993/1‑69 (MQ=255) cAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCaataa < 1:126378/69‑1 (MQ=255) cAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCaataa > 1:2422368/1‑69 (MQ=255) cAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCaataa < 1:51876/69‑1 (MQ=255) cAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCaataa > 1:387273/1‑69 (MQ=255) cAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCaataa < 1:2702751/69‑1 (MQ=255) cAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCaataa < 1:2467466/69‑1 (MQ=255) cAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCaataa < 1:2659590/69‑1 (MQ=255) cAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCaataa < 1:263522/69‑1 (MQ=255) cAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCaataa < 1:155821/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑tt > 1:1975237/1‑36 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCGGGCATGCTATTGAGCAGCAATAATGCa < 1:910726/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa > 1:2764885/1‑69 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa < 1:3119724/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa > 1:793140/1‑69 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa < 1:1177229/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa < 1:2340938/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa < 1:2640939/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa < 1:1654447/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa < 1:2876748/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa < 1:2893793/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa > 1:2433806/1‑69 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa < 1:1801719/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa < 1:3393746/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa < 1:2563103/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa < 1:1490343/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa < 1:3105387/69‑1 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCa > 1:3116691/1‑69 (MQ=255) taaatGGCGATTTTATTGCGGTAATGCTCAGCTG‑TCTTTTCCGGCATGCTATTGAGCAGCAATAATGCa > 1:856521/1‑69 (MQ=255) tGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCATAtt < 1:3267580/69‑1 (MQ=255) tGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCATAtt < 1:815589/69‑1 (MQ=255) | ATGTCCCCTTGCTGGGTTTGTGGAACCTCGATGCCTTTTTTCTGATACCAGTGCAAATAAATGGCGATTTTATTGCGGTAATGCTCAGCTG‑TTTTTTCCGGCATGCTATTGAGCAGCAATAATGCATATT > minE/390418‑390547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |