| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 395,051 | C→T | 20.8% | D134D (GAC→GAT) | ahpF → | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 395,051 | 0 | C | T | 20.8% | 117.3 / 19.9 | 81 | D134D (GAC→GAT) | ahpF | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (24/40); new base T (3/14); total (27/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
TTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACA > minE/394993‑395103 | ttGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgtggtg > 1:2199103/1‑65 (MQ=255)ttGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgtggtg > 1:536600/1‑65 (MQ=255)ttGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgtggtg > 1:3055539/1‑65 (MQ=255) gACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGgcg > 1:1361163/1‑69 (MQ=255) gACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGgcg > 1:2010998/1‑69 (MQ=255) gACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGgcg > 1:764227/1‑69 (MQ=255) gACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGgcg > 1:1339214/1‑69 (MQ=255) gACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGgcg > 1:1337573/1‑69 (MQ=255) gACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGgcg > 1:111922/1‑69 (MQ=255) gTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGa < 1:967410/69‑1 (MQ=255) gTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGa < 1:3337282/69‑1 (MQ=255) gTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGa < 1:1113413/69‑1 (MQ=255) gTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGa < 1:2617159/69‑1 (MQ=255) gATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAAc > 1:2899119/1‑69 (MQ=255) ttttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:3221177/51‑1 (MQ=255) ttttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:215493/51‑1 (MQ=255) ttttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:3326473/51‑1 (MQ=255) ttttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:2327190/51‑1 (MQ=255) ttttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:2248295/51‑1 (MQ=255) ttttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:3155220/51‑1 (MQ=255) ttttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:311159/51‑1 (MQ=255) ttttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:461325/51‑1 (MQ=255) ttttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:1081468/51‑1 (MQ=255) ttttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:997816/51‑1 (MQ=255) ttttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:2933330/51‑1 (MQ=255) ttttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:1500825/51‑1 (MQ=255) ttttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:1433445/51‑1 (MQ=255) tttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGATgt < 1:558314/50‑1 (MQ=255) tttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCt < 1:1004612/68‑1 (MQ=255) tttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCt < 1:3127400/68‑1 (MQ=255) tttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCt < 1:1001816/68‑1 (MQ=255) tttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCt < 1:2756990/68‑1 (MQ=255) tttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCt < 1:2977409/68‑1 (MQ=255) tttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCt < 1:2276636/68‑1 (MQ=255) tttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCt < 1:3018386/68‑1 (MQ=255) tttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCt < 1:2245274/68‑1 (MQ=255) tttGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCt < 1:222592/68‑1 (MQ=255) ttCGAAACCTATTACTCGCTCTCTTGCGACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAg < 1:2535466/68‑1 (MQ=255) ttCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTg < 1:2848960/57‑1 (MQ=255) ttCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTg < 1:2973116/57‑1 (MQ=255) ttCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTg < 1:1178505/57‑1 (MQ=255) ttCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAg < 1:2752215/68‑1 (MQ=255) tCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCg < 1:2573423/69‑1 (MQ=255) tCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCg < 1:2799371/69‑1 (MQ=255) tCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCg < 1:2803433/69‑1 (MQ=255) cTATTACTCGCTCTCTTGCCACAACTGCCCGGACgtggt > 1:403400/1‑39 (MQ=255) cTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTg > 1:2821318/1‑49 (MQ=255) cTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTg > 1:2589815/1‑67 (MQ=255) cTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGaa > 1:400489/1‑69 (MQ=255) cTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGaa > 1:67052/1‑69 (MQ=255) cTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGaa > 1:78090/1‑69 (MQ=255) cTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGaa > 1:99838/1‑69 (MQ=255) cTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGaa > 1:2933158/1‑69 (MQ=255) cTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGa > 1:1866649/1‑68 (MQ=255) aCTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCt < 1:2317670/49‑1 (MQ=255) aCTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCt < 1:3116760/49‑1 (MQ=255) aCTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCt < 1:3116809/49‑1 (MQ=255) aCTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCt < 1:2452996/49‑1 (MQ=255) tCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcgcg < 1:2933676/68‑1 (MQ=255) tCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcgcg < 1:583159/68‑1 (MQ=255) tCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcgcg < 1:2186760/68‑1 (MQ=255) tCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcgcg < 1:114137/68‑1 (MQ=255) tCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcgcg < 1:2614025/68‑1 (MQ=255) tCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcgcg < 1:2981697/68‑1 (MQ=255) cGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCg < 1:2972117/54‑1 (MQ=255) cGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCg < 1:321371/54‑1 (MQ=255) cGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCg < 1:3248412/54‑1 (MQ=255) cGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCg < 1:2722718/54‑1 (MQ=255) cGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCg < 1:1969936/54‑1 (MQ=255) cGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcgcg < 1:1372556/67‑1 (MQ=255) cGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCcgcg < 1:2629355/67‑1 (MQ=255) gCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATc < 1:234343/62‑1 (MQ=255) gCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATc < 1:1953413/62‑1 (MQ=255) gCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGcaca > 1:180566/1‑69 (MQ=255) gCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGcaca > 1:1098600/1‑69 (MQ=255) gCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGcaca > 1:1932242/1‑69 (MQ=255) gCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGcaca > 1:2269354/1‑69 (MQ=255) gCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGcaca > 1:2292119/1‑69 (MQ=255) gCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGcaca > 1:3323938/1‑69 (MQ=255) gCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGcaca > 1:3185588/1‑69 (MQ=255) gCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGcac > 1:2607337/1‑68 (MQ=255) | TTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACA > minE/394993‑395103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |