| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 395,924 | T→A | 57.0% | V425V (GTT→GTA) | ahpF → | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 395,924 | 0 | T | A | 57.0% | 26.7 / 74.7 | 72 | V425V (GTT→GTA) | ahpF | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (23/8); new base A (24/17); total (47/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGTCGACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATCGTGTCAGCGGCGATATTCACAACATCGAACTGGCCGGTATTTTCG > minE/395863‑395988 | aCGTCGACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTgg < 1:1216574/69‑1 (MQ=255)aCGTCGACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTgg < 1:292407/69‑1 (MQ=255)aCGTCGACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTgg < 1:34085/69‑1 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:1765000/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:2459522/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:1787063/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:2158087/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:2329571/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:263725/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:3136478/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:3252835/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:391265/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:5333/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:587102/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:66765/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:883279/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:981766/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:157335/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:1411579/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:1354717/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:1138839/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:1331847/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAatat > 1:1525377/1‑69 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAata > 1:1611394/1‑68 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAata > 1:974442/1‑68 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGCAAAGTCGTAGGTCTGGAata > 1:3418258/1‑68 (MQ=255) gACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGTAGAAAAGTCGTAGGTCTGGAata > 1:2933372/1‑68 (MQ=255) attattCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAata > 1:502304/1‑65 (MQ=255) attCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGAt > 1:2213818/1‑69 (MQ=255) attCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGAt > 1:1413265/1‑69 (MQ=255) attCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGAt > 1:3431500/1‑69 (MQ=255) attCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGAt > 1:2396844/1‑69 (MQ=255) attCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGAt > 1:1882467/1‑69 (MQ=255) attCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGAt > 1:1548073/1‑69 (MQ=255) attCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGAt > 1:3061261/1‑69 (MQ=255) cTGAATGCGCAAACCACGGAAGTTAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATCgt > 1:2774064/1‑69 (MQ=255) cgcAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATCGTGTCAgcg > 1:2300311/1‑69 (MQ=255) cgcAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATCGTGTCAgcg > 1:5787/1‑69 (MQ=255) cgcAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATCGTGTCAgcg > 1:3135382/1‑69 (MQ=255) cgcAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATCGTGTCAgcg > 1:1431552/1‑69 (MQ=255) cgcAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATCGTGTCAgcg > 1:298342/1‑69 (MQ=255) cgcAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATCGTGTCAgc > 1:813544/1‑68 (MQ=255) cgcAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATCGTGTAAgcg > 1:3389951/1‑69 (MQ=255) aaaGGCGACGGCAGCAAAGTCGTTGGTCTGGATTATCGAGATCGTGTCAGCGGCGATATTcac < 1:2133979/63‑1 (MQ=255) aaaGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATCGTGTCAGCGGCGATATTcac < 1:1062452/63‑1 (MQ=255) aaaGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATCGTGTCAGCGGCGATATTcac < 1:2634941/63‑1 (MQ=255) aaaGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATAGTGTCAGCGGCGATATTCA‑AACATCg > 1:508857/1‑69 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATct < 1:287758/68‑2 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:1493446/68‑1 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:1758742/68‑1 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:1873356/68‑1 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:1103461/68‑1 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:2075539/68‑1 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:1110828/68‑1 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:2130336/68‑1 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:2180873/68‑1 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:2295345/68‑1 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:353815/68‑1 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:2406819/68‑1 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:3340196/68‑1 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:3311259/68‑1 (MQ=255) aGGCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:260662/68‑1 (MQ=255) aGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATCGTGTCAGCGGCGATATTCACAACATCg < 1:1319405/68‑1 (MQ=255) ggCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:2310670/67‑1 (MQ=255) ggCGACGGTAGCAAAGTCGTAGGTCTGGAATATCGCGATCGTGTCAGCGGCAATATTCACAACATCg < 1:2688288/67‑1 (MQ=255) agcaAAGTCGTTGGTCTGGAATATCGAGATCGTGTCAGCGGCGATATTCACAACATCGAACTGGCCGGt > 1:3265869/1‑69 (MQ=255) agcaAAGTCGTTGGTCTGGAATATCGAGATCGTGTCAGCGGCGATATTCACAACATCGAACTGGCCGGt < 1:3044397/69‑1 (MQ=255) aaaGTCGTTGGTCTGGAATATCGAGATCGTGTCAGCGGCGATATTCACAACATCGAACTGGCCGGTAtt > 1:2168177/1‑69 (MQ=255) aaaGTCGTTGGTCTGGAATATCGAGATCGTGTCAGCGGCGATATTCACAACATCGAACTCGCCGGTa > 1:338217/1‑67 (MQ=255) tCGTTGGTCTGGAATATCGAGATCGTGTCAGCGGCGATATTCACAACATCGAACTGGCCGGTATTTTCg > 1:1039387/1‑69 (MQ=255) tCGTTGGTCTGGAATATCGAGATCGTGTCAGCGGCGATATTCACAACATCGAACTGGCCGGTATTTTCg > 1:1398758/1‑69 (MQ=255) tCGTTGGTCTGGAATATCGAGATCGTGTCAGCGGCGATATTCACAACATCGAACTGGCCGGTATTTTCg > 1:3243855/1‑69 (MQ=255) | ACGTCGACATTATTCTGAATGCGCAAACCACGGAAGTGAAAGGCGACGGCAGCAAAGTCGTTGGTCTGGAATATCGAGATCGTGTCAGCGGCGATATTCACAACATCGAACTGGCCGGTATTTTCG > minE/395863‑395988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |