| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 396,089 | G→C | 39.1% | V480V (GTG→GTC) | ahpF → | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 396,089 | 0 | G | C | 39.1% | 56.7 / 87.1 | 92 | V480V (GTG→GTC) | ahpF | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (27/29); new base C (24/12); total (51/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
GCAGTCGAACGTAACCGCATGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGATCAT > minE/396030‑396142 | gcAGTCGAACGTAACCGCATGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCgtg < 1:992630/69‑1 (MQ=255)gcAGTCGAACGTAACCGCATGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCgtg < 1:3120095/69‑1 (MQ=255) aaCCGCATGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGt > 1:3196385/1‑69 (MQ=255) aTGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTCAAAGGCGTTTTCGCAGCGGGTGACTg < 1:332682/68‑1 (MQ=255) aTGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTg < 1:1995968/68‑1 (MQ=255) aTGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTg < 1:2159463/68‑1 (MQ=255) aTGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTg < 1:2511194/68‑1 (MQ=255) aTGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTg < 1:2824238/68‑1 (MQ=255) aTGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTg < 1:2983300/68‑1 (MQ=255) aTGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTg < 1:1549285/68‑1 (MQ=255) aTGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTg < 1:325184/68‑1 (MQ=255) aTGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTg < 1:966564/68‑1 (MQ=255) aTGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTg < 1:50828/68‑1 (MQ=255) aTGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTg < 1:514569/68‑1 (MQ=255) tGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTg < 1:1468248/67‑1 (MQ=255) agaTTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACgg > 1:2101046/1‑69 (MQ=255) agaTTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACgg > 1:2270499/1‑69 (MQ=255) agaTTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACgg > 1:2384688/1‑69 (MQ=255) agaTTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACgg > 1:904273/1‑69 (MQ=255) agaTTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACgg > 1:760549/1‑69 (MQ=255) agaTTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACgg > 1:2939479/1‑69 (MQ=255) agaTTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACgg > 1:3064457/1‑69 (MQ=255) agaTTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACgg > 1:3140458/1‑69 (MQ=255) agaTTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACgg > 1:1965773/1‑69 (MQ=255) agaTTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACgg > 1:1972805/1‑69 (MQ=255) agaTTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACgg > 1:419296/1‑69 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCgtg > 1:1025569/1‑40 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCgtg > 1:430471/1‑40 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCgtg < 1:3166896/40‑1 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCgtg < 1:3207841/40‑1 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCgtg < 1:1315722/40‑1 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCgtg < 1:1953820/40‑1 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCgtg < 1:1855114/40‑1 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCgtg < 1:1511687/40‑1 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCgtg < 1:1836120/40‑1 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCgtg < 1:1240549/40‑1 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTc > 1:1260186/1‑68 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTc > 1:1783603/1‑68 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTc > 1:2703941/1‑68 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTc > 1:266362/1‑68 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTc > 1:639401/1‑68 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTc > 1:1991011/1‑68 (MQ=255) tATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTc > 1:1514933/1‑68 (MQ=255) aTCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCg > 1:3348782/1‑69 (MQ=255) aTCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCg > 1:173675/1‑69 (MQ=255) aTCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCg > 1:1000461/1‑69 (MQ=255) tCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCg > 1:2492512/1‑68 (MQ=255) cATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGt > 1:1351545/1‑68 (MQ=255) gcgaaatgcgaaaCCAACGTGATAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:1439430/69‑1 (MQ=255) gcgaaatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:2811356/69‑1 (MQ=255) gcgaaatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:1564500/69‑1 (MQ=255) gcgaaatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:487979/69‑1 (MQ=255) gcgaaatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:2998293/69‑1 (MQ=255) gcgaaatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:1668666/69‑1 (MQ=255) gcgaaatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:2976565/69‑1 (MQ=255) gcgaaatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:2658401/69‑1 (MQ=255) gcgaaatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:209283/69‑1 (MQ=255) gcgaaatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:899107/69‑1 (MQ=255) gcgaaatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:2094851/69‑1 (MQ=255) gcgaaatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:2596957/69‑1 (MQ=255) gcgaaatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:182754/69‑1 (MQ=255) cgaaatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAg < 1:710940/68‑1 (MQ=255) aatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatca < 1:2561284/69‑1 (MQ=255) aatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatca < 1:380950/69‑1 (MQ=255) aatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatca < 1:379630/69‑1 (MQ=255) aatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatca < 1:219414/69‑1 (MQ=255) aatgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatca < 1:2863245/69‑1 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:1090093/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:924186/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:48644/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:982861/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:118493/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:1268368/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:81309/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:1220449/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:1566629/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:375055/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:370844/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:1406433/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:3176380/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:3175461/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:3120472/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:1600591/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:2778303/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:2656875/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:2655772/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:2607537/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:2575917/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:2360316/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:1936959/1‑68 (MQ=255) aatgcgaaaCCAACGTCAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatc > 1:2028460/1‑68 (MQ=255) atgcgaaaCCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGatcat > 1:2737486/1‑69 (MQ=255) | GCAGTCGAACGTAACCGCATGGGCGAGATTATCATTGATGCGAAATGCGAAACCAACGTGAAAGGCGTGTTCGCAGCGGGTGACTGTACGACGGTTCCGTACAAGCAGATCAT > minE/396030‑396142 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |