| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 401,428 | A→G | 24.6% | T36T (ACT→ACC) | dacA ← | D‑alanyl‑D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 401,428 | 0 | A | G | 24.6% | 100.7 / 29.4 | 85 | T36T (ACT→ACC) | dacA | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base A (30/34); new base G (12/9); total (42/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTG > minE/401366‑401491 | ccGGAGTTGTAGTCAATCAGGATGTAGGACTCCGCGTCGATCTGCGGTACACCCGGGATCATGGtttt < 1:2872013/68‑1 (MQ=255)ccGGAGTTGTAGTCAATCAGGATGTAGGACTCCGCGTCGATCTGCGGTACACCCGGGATCATGGtttt < 1:2976528/68‑1 (MQ=255)ccGGAGTTGTAGTCAATCAGGATGTAGGACTCCGCGTCGATCTGCGGTACACCCGGGATCATGGtttt < 1:773939/68‑1 (MQ=255)ccGGAGTTGTAGTCAATCAGGATGTAGGACTCCGCGTCGATCTGCGGTACACCCGGGATCATGGtttt < 1:3086921/68‑1 (MQ=255)ccGGAGTTGTAGTCAATCAGGATGTAGGACTCCGCGTCGATCTGCGGTACACCCGGGATCATGGtttt < 1:1257569/68‑1 (MQ=255)ccGGAGTTGTAGTCAATCAGGATGTAGGACTCCGCGTCGATCTGCGGTACACCCGGGATCATGGtttt < 1:1930588/68‑1 (MQ=255)ccGGAGTTGTAGTCAATCAGGATGTAGGACTCCGCGTCGATCTGCGGTACACCCGGGATCATGGtttt < 1:3398054/68‑1 (MQ=255)ccGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGtttt < 1:3183972/68‑1 (MQ=255)ccGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGtttt < 1:2267754/68‑1 (MQ=255) tCAATCAGGATGTAGGACTCCGCGTCGATCTGCGGTACACCCGGGATCATGGtttt < 1:2222969/56‑1 (MQ=255) cAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCAt > 1:681262/1‑69 (MQ=255) cAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCAt > 1:1097863/1‑69 (MQ=255) gATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCTtg < 1:1486241/69‑1 (MQ=255) gATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg < 1:1934851/69‑1 (MQ=255) gATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg < 1:1889922/69‑1 (MQ=255) gATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg < 1:1996447/69‑1 (MQ=255) gATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg < 1:1801745/69‑1 (MQ=255) gATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg < 1:2610337/69‑1 (MQ=255) aTGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg > 1:2547085/1‑68 (MQ=255) aTGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg > 1:262790/1‑68 (MQ=255) aTGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg > 1:2528988/1‑68 (MQ=255) aTGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg > 1:3034184/1‑68 (MQ=255) aTGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg > 1:368304/1‑68 (MQ=255) aTGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg > 1:427020/1‑68 (MQ=255) aTGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg > 1:479133/1‑68 (MQ=255) aTGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg > 1:999335/1‑68 (MQ=255) aTGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg < 1:2210117/68‑1 (MQ=255) aTGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg > 1:1047947/1‑68 (MQ=255) aTGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg > 1:1488941/1‑68 (MQ=255) aTGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg > 1:126329/1‑68 (MQ=255) aTGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg > 1:1726294/1‑68 (MQ=255) tAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGa > 1:1987735/1‑46 (MQ=255) tAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGa < 1:3189211/46‑1 (MQ=255) tAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGa < 1:3299850/46‑1 (MQ=255) tAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGa < 1:369675/46‑1 (MQ=255) tAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGa > 1:1192388/1‑46 (MQ=255) tAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGa > 1:1778975/1‑46 (MQ=255) tAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGa > 1:1853804/1‑46 (MQ=255) tAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGa < 1:2518791/46‑1 (MQ=255) tAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGa < 1:1164693/46‑1 (MQ=255) tAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGa < 1:839402/46‑1 (MQ=255) tAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGa > 1:1945713/1‑46 (MQ=255) tAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGa > 1:2049087/1‑46 (MQ=255) cTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGat > 1:325231/1‑42 (MQ=255) cTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgcag > 1:359702/1‑65 (MQ=255) cTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCaga < 1:1017080/69‑1 (MQ=255) cTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCaga < 1:1536855/69‑1 (MQ=255) cTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCaga < 1:9134/69‑1 (MQ=255) cTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCaga < 1:1415038/69‑1 (MQ=255) tCCGCGTCGATCTGCGGTACACCCGGGATCATGGtttt < 1:491049/38‑1 (MQ=38) cGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAgaga > 1:1930725/1‑68 (MQ=255) cGCATCGATCTGCGGTAAACCCGGGATCATATTTTTGATATTCAGGTCATCGGCAtg < 1:2636636/57‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgc < 1:2185856/54‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgc < 1:1415819/54‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgc < 1:297265/54‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgc < 1:1336651/54‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgc < 1:240817/54‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgc < 1:1952906/54‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgc < 1:2222237/54‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgc < 1:750955/54‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgc < 1:2547161/54‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgc < 1:2227682/54‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGc < 1:1996980/69‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGc > 1:3127346/1‑69 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGc < 1:2179228/69‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGc < 1:2306253/69‑1 (MQ=255) gATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGc > 1:2648296/1‑69 (MQ=255) aTCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgc < 1:891409/53‑1 (MQ=255) aTCATGGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATaa > 1:508060/1‑46 (MQ=255) aTCATGGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATaa > 1:2169818/1‑46 (MQ=255) aTCATGGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATaa > 1:873289/1‑46 (MQ=255) aTCATGGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATaa > 1:694140/1‑46 (MQ=255) aTCATGGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATaa > 1:1469739/1‑46 (MQ=255) aTCATGGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATaa > 1:2668586/1‑46 (MQ=255) aTCATGGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATaa > 1:1577574/1‑46 (MQ=255) aTCATGGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATaa > 1:2892526/1‑46 (MQ=255) aTCATGGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATaa > 1:2973005/1‑46 (MQ=255) aTCATGGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATaa > 1:3420125/1‑46 (MQ=255) aTCATGGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATaa > 1:3174923/1‑46 (MQ=255) aTCATGGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATa > 1:2411842/1‑45 (MQ=255) aTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCgtggtg > 1:2753524/1‑69 (MQ=255) aTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCgtggtg > 1:1771344/1‑69 (MQ=255) aTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCgtggtg > 1:2920040/1‑69 (MQ=255) aTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCgtggtg > 1:860886/1‑69 (MQ=255) aTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCgtggtg > 1:1537052/1‑69 (MQ=255) | CCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTG > minE/401366‑401491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |