| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 48,236 | C→T | 46.8% | S222N (AGC→AAC) | apaH ← | diadenosine tetraphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 48,236 | 0 | C | T | 46.8% | 10.2 / 65.1 | 79 | S222N (AGC→AAC) | apaH | diadenosine tetraphosphatase |
| Reads supporting (aligned to +/- strand): ref base C (17/25); new base T (22/15); total (39/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01 | |||||||||||
CAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCG > minE/48172‑48302 | cAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGt > 1:1135835/1‑68 (MQ=255)cAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTa > 1:1918757/1‑69 (MQ=255)cAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTa > 1:2042071/1‑69 (MQ=255) tatataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCa < 1:966704/69‑1 (MQ=255) tatataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCa < 1:896332/69‑1 (MQ=255) tatataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCa < 1:3406277/69‑1 (MQ=255) tatataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCa < 1:2959823/69‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTg < 1:3243518/58‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTg < 1:2020744/58‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTg < 1:2444032/58‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTg < 1:2526908/58‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTg < 1:2600932/58‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTg < 1:2853037/58‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTg < 1:3355499/58‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTg < 1:1430586/58‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTg < 1:370181/58‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTg < 1:1255120/58‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTg < 1:794952/58‑1 (MQ=255) ataCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:234302/64‑1 (MQ=255) ataCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:3328017/64‑1 (MQ=255) ataCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:2804061/64‑1 (MQ=255) ataCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:2771941/64‑1 (MQ=255) ataCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:2383250/64‑1 (MQ=255) ataCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:1007013/64‑1 (MQ=255) ataCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:829272/64‑1 (MQ=255) ataCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:904914/64‑1 (MQ=255) ataCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:1836437/64‑1 (MQ=255) ataCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:1788736/64‑1 (MQ=255) ataCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:1596732/64‑1 (MQ=255) ataCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:1447196/64‑1 (MQ=255) ataCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:1457016/64‑1 (MQ=255) taCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:86557/63‑1 (MQ=255) taCCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTActctt < 1:1499381/63‑1 (MQ=255) taCCTTACGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGc > 1:1965055/1‑67 (MQ=255) tCCAGCGATGGCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:944285/1‑51 (MQ=255) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:1631680/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:1748688/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:2714162/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:2790819/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:1567823/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:2811699/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:3036721/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:1458945/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:3344896/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:1302562/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:42269/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:650285/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:886419/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:2562890/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:1639453/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:2422598/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:1704622/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:1677291/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:2115234/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:2580052/1‑51 (MQ=38) tCCAGCGATGCCCAGTGACCAAAGGCGATGTTGTACTCTTCAGCGACgggg > 1:2283186/1‑51 (MQ=38) ccAGCGCTGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACg > 1:1058/1‑69 (MQ=255) ccAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACg > 1:2180961/1‑69 (MQ=255) ccAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACg > 1:1938307/1‑69 (MQ=255) ccAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACg > 1:1171706/1‑69 (MQ=255) ccAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACg > 1:516598/1‑69 (MQ=255) ccAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACg > 1:1648728/1‑69 (MQ=255) ccAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACg > 1:2398717/1‑69 (MQ=255) gATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTc < 1:3336682/69‑1 (MQ=255) gATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTc < 1:1635084/69‑1 (MQ=255) gATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTc < 1:1393088/69‑1 (MQ=255) gATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTc < 1:307255/69‑1 (MQ=255) gATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTc < 1:390271/69‑1 (MQ=255) gATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTc < 1:913657/69‑1 (MQ=255) gATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTc < 1:1475502/69‑1 (MQ=255) gATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTc < 1:2919386/69‑1 (MQ=255) gATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTc < 1:1987867/69‑1 (MQ=255) aCCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAgg > 1:1015809/1‑69 (MQ=255) aCCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAgg > 1:164949/1‑69 (MQ=255) ggCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGc < 1:2350760/66‑1 (MQ=255) ggCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGc > 1:2339667/1‑66 (MQ=255) gCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCg > 1:1946255/1‑68 (MQ=255) gCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCg > 1:1022228/1‑68 (MQ=255) gCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCg > 1:1018326/1‑68 (MQ=255) | CAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCG > minE/48172‑48302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |