| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 405,082 | A→G | 11.9% | I236I (ATT→ATC) | mrdA ← | transpeptidase involved in peptidoglycan synthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 405,082 | 0 | A | G | 11.9% | 105.1 / 3.5 | 58 | I236I (ATT→ATC) | mrdA | transpeptidase involved in peptidoglycan synthesis |
| Reads supporting (aligned to +/- strand): ref base A (21/30); new base G (3/4); total (24/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
TGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCT > minE/405021‑405145 | tGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAacac > 1:2549629/1‑69 (MQ=255) tCCAGCTTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCa < 1:1085537/69‑1 (MQ=255) tCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGccaa < 1:2715859/69‑3 (MQ=255) tCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCa < 1:1198741/69‑1 (MQ=255) tCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCa < 1:1531080/69‑1 (MQ=255) tCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCa < 1:1454525/69‑1 (MQ=255) tCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCa < 1:562947/69‑1 (MQ=255) tCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCa < 1:1745190/69‑1 (MQ=255) gTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGGATAACACGCCCACGgtt > 1:631066/1‑68 (MQ=255) gTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGGATAACACGCCCACGgtt > 1:1677129/1‑68 (MQ=255) gTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGGATAACACGCCCACGgtt > 1:2897255/1‑68 (MQ=255) aaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGgttgttt < 1:596580/64‑2 (MQ=255) aaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTa < 1:3192724/64‑1 (MQ=255) aaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTa > 1:2094713/1‑64 (MQ=255) aaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTa < 1:2163206/64‑1 (MQ=255) aaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTa < 1:2288771/64‑1 (MQ=255) aaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTa < 1:3370327/64‑1 (MQ=255) aaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTa < 1:849967/64‑1 (MQ=255) aaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTa > 1:1298884/1‑64 (MQ=255) aaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTa < 1:1189251/64‑1 (MQ=255) aaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTa > 1:1177607/1‑64 (MQ=255) aaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTa < 1:1036159/64‑1 (MQ=255) aaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCCAATAACACGCCCACGGTTGTTa < 1:3031150/64‑1 (MQ=255) aaTATCGTGTCCGGCTTGCGGGGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTa < 1:1326510/64‑1 (MQ=255) atCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAAc < 1:398877/69‑1 (MQ=255) atCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAAc < 1:368954/69‑1 (MQ=255) atCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAAc < 1:371572/69‑1 (MQ=255) tCGTGTCCGGCTTGCGGTGGTACTTCTTTTAATTGGCGAATAACACGCCCACGGTTGTTAACTTCAAcc < 1:521812/69‑1 (MQ=255) tCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAAcc < 1:3206307/69‑1 (MQ=255) tCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAAcc < 1:662439/69‑1 (MQ=255) tCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAAcc < 1:578613/69‑1 (MQ=255) tCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAAcc < 1:658391/69‑1 (MQ=255) tCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAAcc < 1:1363169/69‑1 (MQ=255) tCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAAc < 1:458853/68‑1 (MQ=255) gtgtCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTa < 1:3188415/58‑1 (MQ=255) gtgtCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAAc < 1:375501/66‑1 (MQ=255) gCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTa < 1:1207088/51‑1 (MQ=255) gtACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGAc > 1:2842506/1‑69 (MQ=255) cttcttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCg > 1:1020009/1‑68 (MQ=255) cttcttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCg > 1:2818732/1‑68 (MQ=255) cttcttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCg > 1:1602781/1‑68 (MQ=255) ctgcttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCg > 1:1465087/4‑68 (MQ=255) cttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCaa > 1:509772/1‑42 (MQ=255) cttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTgc > 1:3369255/1‑68 (MQ=255) cttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTgc > 1:1010484/1‑68 (MQ=255) cttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTgc > 1:1048890/1‑68 (MQ=255) cttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTgc > 1:1090364/1‑68 (MQ=255) cttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTgc > 1:1206875/1‑68 (MQ=255) cttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTgc > 1:1641340/1‑68 (MQ=255) cttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTgc > 1:180950/1‑68 (MQ=255) cttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTgc > 1:2303891/1‑68 (MQ=255) cttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTgc > 1:2635348/1‑68 (MQ=255) cttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTgc > 1:2744662/1‑68 (MQ=255) cttTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTgc > 1:2795868/1‑68 (MQ=255) tGGCGGATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACGTCt < 1:2448613/69‑1 (MQ=255) tGGCGGATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACGTCt < 1:686059/69‑1 (MQ=255) tGGCGGATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACGTCt < 1:3001841/69‑1 (MQ=255) tGGCGGATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACGTCt < 1:937109/69‑1 (MQ=255) | TGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCT > minE/405021‑405145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |