| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 407,336 | A→G | 24.0% | D48D (GAT→GAC) | cobC ← | predicted alpha‑ribazole‑5'‑P phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 407,336 | 0 | A | G | 24.0% | 68.8 / 17.8 | 58 | D48D (GAT→GAC) | cobC | predicted alpha‑ribazole‑5'‑P phosphatase |
| Reads supporting (aligned to +/- strand): ref base A (25/19); new base G (12/2); total (37/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.16e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
GGTCACTGAGAACCAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT > minE/407275‑407398 | ggTCACTGAGAACCAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAgg < 1:1127874/69‑1 (MQ=255) gTCACTGAGAACCAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGa < 1:2611610/69‑1 (MQ=255) gTCACTGAGAACCAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGa < 1:582112/69‑1 (MQ=255) gTCACTGAGAACCAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGa < 1:1253770/69‑1 (MQ=255) gTCACTGAGAACCAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGa < 1:2673964/69‑1 (MQ=255) gTCACTGAGAACCAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGa < 1:2426907/69‑1 (MQ=255) gTCACTGAGAACCAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGa < 1:2901711/69‑1 (MQ=255) aCTGAGAACCAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAac < 1:1958047/69‑1 (MQ=255) aCTGAGAACCAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAac < 1:2219240/69‑1 (MQ=255) ccAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGt > 1:1679526/1‑68 (MQ=255) ccAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGt > 1:3092368/1‑68 (MQ=255) ccAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGt > 1:1430481/1‑68 (MQ=255) cgcgcgGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTagcagc < 1:2381994/69‑1 (MQ=255) gcgcgGTATGCTGTGCCCGTTCGAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTAGCAGCg > 1:2741610/1‑69 (MQ=255) gcgcgGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTagcagc < 1:2646383/68‑1 (MQ=255) gcgcgGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTagcagc > 1:3287816/1‑68 (MQ=255) gcgcgGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTAGCAGCg > 1:1422430/1‑69 (MQ=255) gcgcgGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTAGCAGCg > 1:259822/1‑69 (MQ=255) gcgcgGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTAGCAGCg > 1:1133003/1‑69 (MQ=255) gcgcgGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTAGCAGCg > 1:3375130/1‑69 (MQ=255) gcgcgGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTAGCAGCg > 1:1318976/1‑69 (MQ=255) gcgcgGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTAGCAGCg > 1:2783692/1‑69 (MQ=255) gcgcgGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTAGCAGCg > 1:2937462/1‑69 (MQ=255) gcgcgGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTAGCAGCg > 1:2991959/1‑69 (MQ=255) gcgcgGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTAGCAGCg > 1:1961536/1‑69 (MQ=255) gcgcgGTATGCTGTGCCCGTGCCAGTTCACTGCATAAAACCAGGTCAAAGGAAACATCATGTAGCAGCg > 1:3052592/1‑69 (MQ=255) cTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGAtt > 1:2228324/1‑69 (MQ=255) cTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGAtt > 1:1516843/1‑69 (MQ=255) tCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGc < 1:762777/69‑1 (MQ=255) tCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGc < 1:3157224/69‑1 (MQ=255) tCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGc < 1:3172580/69‑1 (MQ=255) ccAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGc > 1:393517/1‑68 (MQ=255) ccAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGc > 1:3331384/1‑68 (MQ=255) ccAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGc > 1:1780337/1‑68 (MQ=255) aGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTc < 1:357473/68‑1 (MQ=255) cATAAAGCCAGATCAAAGGAAACACCATGTagcagc > 1:443141/1‑36 (MQ=255) cATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGAtt > 1:2389980/1‑47 (MQ=255) cATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTc > 1:696257/1‑59 (MQ=255) cATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTc > 1:1209717/1‑59 (MQ=255) cATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTc > 1:2753721/1‑59 (MQ=255) cATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTc > 1:3146601/1‑59 (MQ=255) cATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTc > 1:1932976/1‑59 (MQ=255) cATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACcgcgc > 1:2548901/1‑69 (MQ=255) cATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACcgcgc > 1:2035066/1‑69 (MQ=255) cATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACcgcgc > 1:2121989/1‑69 (MQ=255) cATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACcgcgc > 1:2188714/1‑69 (MQ=255) cATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACcgcgc > 1:2506150/1‑69 (MQ=255) cATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACcgcgc > 1:2555042/1‑69 (MQ=255) cATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACcgagc > 1:1305491/1‑69 (MQ=255) aaaCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACcgcgcgcg > 1:203799/1‑68 (MQ=255) aaaCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACcgcgcgcg > 1:2883198/1‑68 (MQ=255) aaaCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACcgcgcgcg > 1:1071600/1‑68 (MQ=255) aCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGTTTTTGCGCTTGCTCAATACCGCGCGCGGt < 1:3209984/68‑1 (MQ=255) aCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGt < 1:1968432/68‑1 (MQ=255) aCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGt < 1:3023967/68‑1 (MQ=255) aCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGt < 1:1454125/68‑1 (MQ=255) aCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGt < 1:1313358/68‑1 (MQ=255) aCCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGt < 1:508049/68‑1 (MQ=255) | GGTCACTGAGAACCAGTCGCGCGGTATGCTGTGCCCGTTCCAGTTCACTGCATAAAACCAGATCAAAGGAAACACCATGTAGCAGCGTATGCAGATTTTGCGCTTGCTCAATACCGCGCGCGGT > minE/407275‑407398 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |