Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 412,305 A→G 24.0% H17H (CAT→CAC)  leuS ← leucyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE412,3050AG24.0% 144.9 / 40.2 116H17H (CAT→CAC) leuSleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (51/37);  new base G (18/10);  total (69/47)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

AATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCA  >  minE/412250‑412363
                                                       |                                                          
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:2926950/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:2266909/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:781458/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:2289930/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:30089/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:2711032/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:439500/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:2923115/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:3026472/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:85835/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:1996022/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:2665380/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:868182/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:908210/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                                               >  1:2571118/1‑69 (MQ=255)
aaTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAA‑GAAGCTGTACtt                                               >  1:1240158/1‑68 (MQ=255)
   aCTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTg                                             <  1:1357910/68‑1 (MQ=255)
    cTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTgg                                            >  1:2536698/1‑68 (MQ=255)
    cTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGa                                           >  1:2930144/1‑69 (MQ=255)
    cTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGa                                           >  1:3088246/1‑69 (MQ=255)
    cTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGa                                           >  1:1508095/1‑69 (MQ=255)
    cTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGa                                           >  1:2406249/1‑69 (MQ=255)
    cTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGa                                           >  1:1413300/1‑69 (MQ=255)
    cTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGa                                           >  1:2702690/1‑69 (MQ=255)
    cTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGa                                           >  1:1278677/1‑69 (MQ=255)
      tctcTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGAt                                          >  1:529703/1‑68 (MQ=255)
      tctcTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGAt                                          >  1:2003031/1‑68 (MQ=255)
      tctcTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGAt                                          >  1:3133532/1‑68 (MQ=255)
      tctcTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGAt                                          >  1:359161/1‑68 (MQ=255)
      tctcTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGAt                                          >  1:2875245/1‑68 (MQ=255)
      tctcTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGAt                                          >  1:1389254/1‑68 (MQ=255)
       ctctTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTc                                        >  1:2003614/1‑69 (MQ=255)
       ctctTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTc                                        >  1:1861590/1‑69 (MQ=255)
       ctctTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTc                                        >  1:3173461/1‑69 (MQ=255)
         ctTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTc                                        <  1:315056/67‑1 (MQ=255)
         ctTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTc                                        <  1:3299048/67‑1 (MQ=255)
         ctTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTc                                        <  1:2484536/67‑1 (MQ=255)
         ctTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTc                                        <  1:433193/67‑1 (MQ=255)
         ctTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTc                                        <  1:1651284/67‑1 (MQ=255)
         ctTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCt                                       <  1:58535/68‑1 (MQ=255)
           ttGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTAtc                                    >  1:3136008/1‑69 (MQ=255)
           ttGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTAtc                                    >  1:1961913/1‑69 (MQ=255)
           ttGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTAtc                                    >  1:1574981/1‑69 (MQ=255)
           ttGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTAtc                                    >  1:1532482/1‑69 (MQ=255)
           ttGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTAtc                                    >  1:761992/1‑69 (MQ=255)
           ttGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTAtc                                    >  1:204620/1‑69 (MQ=255)
           ttGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTAtc                                    >  1:1363642/1‑69 (MQ=255)
               tctcGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATctct                                 <  1:2210850/68‑1 (MQ=255)
               tctcGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATctct                                 <  1:1695914/68‑1 (MQ=255)
               tctcGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATctct                                 <  1:1602830/68‑1 (MQ=255)
               tctcGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATctct                                 <  1:1449502/68‑1 (MQ=255)
               tctcGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATctct                                 <  1:3246560/68‑1 (MQ=255)
                          gTTACTTCAAATGTGCTCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCgg                        <  1:967896/66‑1 (MQ=255)
                          gTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCgg                        <  1:2377862/66‑1 (MQ=255)
                          gTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCgg                        <  1:3196756/66‑1 (MQ=255)
                          gTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCgg                        <  1:1391746/66‑1 (MQ=255)
                          gTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCgg                        <  1:2041960/66‑1 (MQ=255)
                          gTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCgg                        <  1:2945052/66‑1 (MQ=255)
                          gTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCgg                        <  1:2979631/66‑1 (MQ=255)
                          gTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCgg                        <  1:1941726/66‑1 (MQ=255)
                          gTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCgg                        <  1:3046948/66‑1 (MQ=255)
                          gTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCgg                        <  1:3387948/66‑1 (MQ=255)
                          gTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCgg                        <  1:1759710/66‑1 (MQ=255)
                           ttACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTAt                     <  1:3322599/68‑1 (MQ=255)
                           ttACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTAt                     <  1:76196/68‑1 (MQ=255)
                                tCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGctct               >  1:2490182/1‑69 (MQ=255)
                                tCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGctct               >  1:1194518/1‑69 (MQ=255)
                                tCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGctct               >  1:856663/1‑69 (MQ=255)
                                tCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGctct               >  1:1748690/1‑69 (MQ=255)
                                tCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGctct               >  1:1890925/1‑69 (MQ=255)
                                tCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGctct               >  1:2071167/1‑69 (MQ=255)
                                tCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGctct               >  1:45221/1‑69 (MQ=255)
                                tCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGctct               >  1:227334/1‑69 (MQ=255)
                                tCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGctct               >  1:2382987/1‑69 (MQ=255)
                                tCAAATGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGctct               >  1:1014573/1‑69 (MQ=255)
                                    aTGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCa           <  1:3332805/69‑1 (MQ=255)
                                    aTGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCa           <  1:2494433/69‑1 (MQ=255)
                                    aTGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCa           <  1:3070064/69‑1 (MQ=255)
                                    aTGTGCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCa           <  1:1394537/69‑1 (MQ=255)
                                    aTGTGCGCTTCTCATCCCAGTGAAGCTGTACCTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCa           <  1:326363/69‑1 (MQ=255)
                                    aTGTGCGCTTCTCATCCCAGTGAAGATGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCa           <  1:3096699/69‑1 (MQ=255)
                                    aTGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCTGGCGGTAt                     <  1:2031225/59‑1 (MQ=255)
                                     tgtgCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCa           <  1:514093/68‑1 (MQ=255)
                                     tgtgCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCa           <  1:1337001/68‑1 (MQ=255)
                                     tgtgCGCTTCTCATCCCAGTGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCa           <  1:380068/68‑1 (MQ=255)
                                     tgtgCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCAt          <  1:2890662/69‑1 (MQ=255)
                                      gtgCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGct                 <  1:2202983/61‑1 (MQ=255)
                                      gtgCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGct                 <  1:1666592/61‑1 (MQ=255)
                                      gtgCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGct                 <  1:1141657/61‑1 (MQ=255)
                                       tgCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCAt          >  1:1780516/1‑67 (MQ=255)
                                       tgCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCAt          >  1:344338/1‑67 (MQ=255)
                                       tgCGCTTCTCATCCCAATGAAGATGTACTTTGGATTCTAtc                                    >  1:966623/1‑41 (MQ=255)
                                        gcgcTTCTCATCCCAATGAAGCTGTACTTTGGATTCt                                       <  1:2385743/37‑1 (MQ=255)
                                            ttCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTAt                     <  1:1589946/51‑1 (MQ=255)
                                             tctcATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCgg                        <  1:1345144/47‑1 (MQ=255)
                                             tctcATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCa  <  1:3278228/69‑1 (MQ=255)
                                             tctcATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCa  <  1:1903333/69‑1 (MQ=255)
                                              ctcATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTg             >  1:2238287/1‑57 (MQ=255)
                                              ctcATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTg             >  1:1801843/1‑57 (MQ=255)
                                                    ccAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTg                   >  1:3356657/1‑45 (MQ=255)
                                                       |                                                          
AATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCA  >  minE/412250‑412363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑