| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 49,231 | A→G | 44.8% | A18A (GCT→GCC) | apaG ← | protein associated with Co2+ and Mg2+ efflux |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 49,231 | 0 | A | G | 44.8% | 21.5 / 96.0 | 116 | A18A (GCT→GCC) | apaG | protein associated with Co2+ and Mg2+ efflux |
| Reads supporting (aligned to +/- strand): ref base A (33/31); new base G (34/18); total (67/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
TCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAACTCTCC > minE/49165‑49296 | tCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGc < 1:513979/69‑1 (MQ=255)tCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGc < 1:2121036/69‑1 (MQ=255)tCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGc < 1:2143061/69‑1 (MQ=255)tCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGc < 1:2471013/69‑1 (MQ=255)tCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGc < 1:911730/69‑1 (MQ=255)tCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGc < 1:764677/69‑1 (MQ=255)tCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGc < 1:690041/69‑1 (MQ=255)tCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGc < 1:3005129/69‑1 (MQ=255)tCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGc < 1:686174/69‑1 (MQ=255)tCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACCTTCATTATCAGGTGAAGATTGAGc < 1:150806/69‑1 (MQ=255) tgATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:168256/68‑1 (MQ=255) aGATTGCTTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:893285/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:1394783/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:872140/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:454463/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:2547613/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:2557333/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:1647673/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:384603/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:1575909/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:1565703/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:1241987/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:3325692/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:3188908/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:1369600/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATg < 1:257111/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATg < 1:292229/69‑1 (MQ=255) aGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATg < 1:506520/69‑1 (MQ=255) gATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGGGCCTCAATg < 1:3345646/68‑1 (MQ=255) tGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAg > 1:1041603/1‑62 (MQ=255) gTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGa > 1:1138505/1‑69 (MQ=255) gTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGa > 1:1157645/1‑69 (MQ=255) gTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTg < 1:1818908/68‑1 (MQ=255) gTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTg < 1:1253518/68‑1 (MQ=255) gTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTg < 1:2263278/68‑1 (MQ=255) tataAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTg < 1:2294124/67‑1 (MQ=255) tataAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGaa > 1:1699779/1‑69 (MQ=255) tataAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGaa > 1:1744598/1‑69 (MQ=255) tataAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGaa > 1:610865/1‑69 (MQ=255) tataAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGaa > 1:1306673/1‑69 (MQ=255) aGCAAAAACGTAACTTTCATTATCAGGTGAAGATTGAGCCCCAATGTAGACGc < 1:2408512/53‑1 (MQ=255) aaaaaCGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGAATaca < 1:1947110/66‑1 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTa > 1:2628616/1‑37 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGaa > 1:66399/1‑48 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGc > 1:917987/1‑68 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:518099/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:517766/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:424045/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:495934/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:2770725/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:1465627/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:1475562/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:718389/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:1523718/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:2677068/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:20180/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:2199275/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:770128/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:2271903/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:2394620/1‑69 (MQ=255) aacgtTCATTATCAGGTGAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:2061938/1‑69 (MQ=255) aacgtTCATTATAAGGTGAAGATTGGGCCTCAATg > 1:2735726/1‑35 (MQ=38) cATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACTCTCGGGGCGAATTg > 1:3140677/1‑68 (MQ=255) cATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTg > 1:1427999/1‑68 (MQ=255) cATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTg > 1:1883919/1‑68 (MQ=255) cATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTg > 1:785111/1‑68 (MQ=255) cATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTg > 1:1665109/1‑68 (MQ=255) cATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTg > 1:2904286/1‑68 (MQ=255) cATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTg > 1:2852713/1‑68 (MQ=255) gAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCg > 1:1909173/1‑47 (MQ=255) gAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCg > 1:186530/1‑47 (MQ=255) gAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCg > 1:919053/1‑47 (MQ=255) gAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCg > 1:2207375/1‑47 (MQ=255) gAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCg > 1:1194406/1‑47 (MQ=255) gAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCg > 1:1857673/1‑47 (MQ=255) gAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCg > 1:2498461/1‑47 (MQ=255) gAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCg > 1:810886/1‑47 (MQ=255) gAAGATTGGGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCg > 1:2834349/1‑47 (MQ=255) gAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCg > 1:3300906/1‑52 (MQ=255) gAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTg < 1:1108331/57‑1 (MQ=255) gAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTg < 1:1409349/57‑1 (MQ=255) gATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAActc < 1:622953/68‑1 (MQ=255) gATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAActc < 1:3157568/68‑1 (MQ=255) gATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAActc < 1:2923046/68‑1 (MQ=255) gATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAActc < 1:2248390/68‑1 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGCACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:927619/1‑66 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:2801670/1‑66 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:1190254/1‑66 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:1646637/1‑66 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:1865274/1‑66 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:2552861/1‑66 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:2559291/1‑66 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:2627808/1‑66 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:388747/1‑66 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:734655/1‑66 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:3029672/1‑66 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:651232/1‑66 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:316189/1‑66 (MQ=255) aTTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAAct > 1:3349854/1‑66 (MQ=255) tGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAACTCTcc > 1:2856152/1‑68 (MQ=255) | TCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCATTATCAGGTGAAGATTGAGCCTCAATGTAGACGCTTTGAACCTGAATACACACTCGGGGCGAATTGATCATCGTTAACTCTCC > minE/49165‑49296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |