| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 413,316 | A→G | 100% | R289R (CGT→CGC) | lnt ← | apolipoprotein N‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 413,316 | 0 | A | G | 79.3% | 30.6 / 2.1 | 24 | R289R (CGT→CGC) | lnt | apolipoprotein N‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base G (1/18); total (3/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.88e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTATCGTAGCGGTTCTGCTTATTGAGACGCGCGTCGACAATCCCGGTTACCAGCGAGCTACCTTTATCACGCAACTCACCGTCCAGTGCTTTGAGGAACGGTTGCT > minE/413248‑413353 | gTATCGTAGCGGTTCTGCTTATTGAGACGCGCGTCGACAATCCCGGTTACCAGCGAGCTACCTTTATCa > 1:692732/1‑69 (MQ=255) tATCGTAGCGGTTCTGCTTATTGAGACGCGCGTCGACAATCCCGGTTACCAGCGAGCTACCTTTATCa > 1:2505720/1‑68 (MQ=255) tATCGTAGCGGTTCTGCTTATTGAGACGCGCGTCGACAATCCCGGTTACCAGCGAGCTACCTTTATCa > 1:3129310/1‑68 (MQ=255) aTCGTAGCGGTTCTGTTTATTGAGACGCGCGTCGACAATCCCGG‑‑ACCAGCGAGCTACCTTTATCGCGCa > 1:748042/1‑69 (MQ=255) cTGCTTATTGAGACGCGCGTCGACAATCCCGGTTACCAGCGAGCTACCTTTATCACGCAACTCACCGTc < 1:363713/69‑1 (MQ=255) gcgcgTCGACAATCCCGGTTACCAGCGAGCTACCTTTATCACGCAACTCACCGTCCAGTGCTTTGAGGa > 1:2018344/1‑69 (MQ=255) gcgcgTCGACAATCCCGGTTACCAGCGAGCTACCTTTATCACGCAACTCACCGTCCAGTGCTTTGAGGa > 1:2294955/1‑69 (MQ=255) cgTCGACAATCCCGGTTACCAGCGAGCTACCTTTATCACGCAACTCACCGTCCAGTGCTTTGAGGAACg < 1:1415370/69‑1 (MQ=255) cgTCGACAATCCCGGTTACCAGCGAGCTACCTTTATCACGCAACTCACCGTCCAGTGCTTTGAGGAACg < 1:2089790/69‑1 (MQ=255) aCCTTTATCGCGCAACTCTCCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:2648144/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:919501/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:1032285/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:790791/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:344507/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:3430976/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:3419718/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:2682686/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:2006545/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:1944563/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:1907775/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:1771658/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:1414616/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:1367520/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:1121884/47‑1 (MQ=255) aCCTTTATCGCGCAACTCACAGTCCAGAGCTTTGAGGAACGGTTGCt < 1:1107102/47‑1 (MQ=255) cTTTATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:525198/45‑1 (MQ=255) tATCGCGCAACTCACCGTCCAGAGCTTTGAGGAACGGTTGCt < 1:395650/42‑1 (MQ=255) | GTATCGTAGCGGTTCTGCTTATTGAGACGCGCGTCGACAATCCCGGTTACCAGCGAGCTACCTTTATCACGCAACTCACCGTCCAGTGCTTTGAGGAACGGTTGCT > minE/413248‑413353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |