| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 413,754 | C→T | 40.9% | L143L (CTG→CTA) | lnt ← | apolipoprotein N‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 413,754 | 0 | C | T | 40.9% | 4.2 / 11.4 | 22 | L143L (CTG→CTA) | lnt | apolipoprotein N‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (7/6); new base T (3/6); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
ATCGGTGCCAGCCCTTTTAACGGGCCATCAATCTGGCTATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCA > minE/413686‑413822 | aTCGGTGCCAGCCCTTTTAACGGGCCATCAATCTGGCTATAGCCGAACTGTAACCACGGGAAGCCGGTc > 1:365569/1‑69 (MQ=255)aTCGGTGCCAGCCCTTTTAACGGGCCATCAATCTGGCTATAGCCGAACTGTAACCACGGGAAGCCGGTc > 1:2000204/1‑69 (MQ=255) ttAACGGGCCATCAATCTGGCTATAGCCGAACTGTAACCACGGGAAGCCTGTTAGTACCCAACcgcg > 1:2211810/1‑67 (MQ=255) ttAACGGGCCATCAATCTGGCTATAGCCGAACTGTAACCACGGGAAGCCTGTTAGTACCCAACCgcgc > 1:1315239/1‑68 (MQ=255) ttAACGGGCCATCAATCTGGCTATAGCCGAACTGTAACCACGGGAAGCCTGTTAGTACCCAACCgcgc > 1:3377681/1‑68 (MQ=255) tAACGGGCCATCAATCTGGCTATAGCCGAACTGTAACCACGGGAAGCCGGTCAg < 1:235062/54‑1 (MQ=255) aaTCTGGCTATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGa < 1:349955/58‑1 (MQ=255) aaTCTGGCTATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCAc > 1:1126059/1‑69 (MQ=255) aaTCTGGCTATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCAc > 1:808474/1‑69 (MQ=255) aaTCTGGCTATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCAc > 1:782014/1‑69 (MQ=255) aaTCTGGCTATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCAc > 1:405499/1‑69 (MQ=255) aaTCTGGCTATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCAc > 1:2049875/1‑69 (MQ=255) aaTCTGGCTATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCAc > 1:3092122/1‑69 (MQ=255) tCTGGCTATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACtt < 1:2753943/69‑1 (MQ=255) gCTATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACt < 1:3050283/64‑1 (MQ=255) gCTATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACt < 1:1629706/64‑1 (MQ=255) tataGCCGAACTGTAACCACGGGAAGCCTGTTAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCa < 1:1133920/67‑1 (MQ=255) tataGCCGAACTGTAACCACGGGAAGCCTGTTAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCa < 1:1582003/67‑1 (MQ=255) tataGCCGAACTGTAACCACGGGAAGCCTGTTAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCa < 1:1472601/67‑1 (MQ=255) tataGCCGAACTGTAACCACGGGAAGCCTGTTAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCa < 1:1360923/67‑1 (MQ=255) tataGCCGAACTGTAACCACGGGAAGCCTGTTAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCa < 1:1252460/67‑1 (MQ=255) gCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGc > 1:851023/1‑69 (MQ=255) cGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGc < 1:1582643/69‑1 (MQ=255) gCCTGTTAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCa < 1:2731093/42‑1 (MQ=38) cAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCa > 1:2323385/1‑69 (MQ=255) cAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCa > 1:864879/1‑69 (MQ=255) | ATCGGTGCCAGCCCTTTTAACGGGCCATCAATCTGGCTATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCA > minE/413686‑413822 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |