| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 418,848 | G→A | 59.2% | E149E (GAG→GAA) | ubiF → | 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 418,848 | 0 | G | A | 59.2% | 24.3 / 50.0 | 71 | E149E (GAG→GAA) | ubiF | 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase |
| Reads supporting (aligned to +/- strand): ref base G (22/7); new base A (8/34); total (30/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
GTGTGCCAGGCTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCA > minE/418793‑418910 | gtgtGCCAGGCTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGt < 1:1545202/68‑1 (MQ=255)gtgtGCCAGGCTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGt < 1:2398002/68‑1 (MQ=255) ccAGGCTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTg > 1:1911494/1‑69 (MQ=255) ccAGGCTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTg > 1:1811590/1‑69 (MQ=255) ccAGGCTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTg > 1:2505494/1‑69 (MQ=255) ccAGGCTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTg > 1:1512364/1‑69 (MQ=255) ccAGGCTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTg > 1:980363/1‑69 (MQ=255) cTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTcg > 1:56166/1‑69 (MQ=255) cTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTcg > 1:2693694/1‑69 (MQ=255) cTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTcg > 1:1943667/1‑69 (MQ=255) cTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTcg > 1:2523600/1‑69 (MQ=255) cTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTc > 1:614945/1‑68 (MQ=255) cTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTc > 1:312049/1‑68 (MQ=255) cTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTc > 1:2922900/1‑68 (MQ=255) gCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTcgcg > 1:335916/1‑68 (MQ=255) ctcTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:479292/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCATGAGCTGGAACTGaa < 1:2342064/38‑1 (MQ=25) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:2188008/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:2633191/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:2450042/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:248450/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:2459705/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:2322501/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:2373467/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:2675457/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:2758391/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:2938048/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:3187700/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:3262071/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:3292803/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:415377/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:419343/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:590279/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:601412/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:759506/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:1229909/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:1397566/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:2144588/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:2143545/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:214197/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:1506118/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:1555435/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:1718347/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:2044598/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:1772537/38‑1 (MQ=37) cacTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:1984055/38‑1 (MQ=37) cacTGCATCGCAATAATGATCTTCAGGAGCTGGAACTGaa < 1:2176633/38‑1 (MQ=25) cacTGCATCGACATAATGATCTTCAGGAGCTGGAACTGaa < 1:1550112/38‑1 (MQ=25) acTGCATCGCCATAATGATCTTCAGGAGCTGGAACTGaa < 1:1682128/38‑1 (MQ=37) cTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGt < 1:455346/68‑1 (MQ=255) cTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGt < 1:454825/68‑1 (MQ=255) cTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGt < 1:360170/68‑1 (MQ=255) gCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGt > 1:473109/1‑68 (MQ=255) gCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTg > 1:2577747/1‑69 (MQ=255) gCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTg > 1:1212384/1‑69 (MQ=255) gCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTg > 1:957811/1‑69 (MQ=255) gCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTg > 1:1419572/1‑69 (MQ=255) gCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTg > 1:2065563/1‑69 (MQ=255) gCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTg > 1:192801/1‑69 (MQ=255) gCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTg > 1:1812324/1‑69 (MQ=255) atggtCTTCAGGAGCTGGAACTGAAGGGCGGTGAAACGATCCTCGCGaa > 1:214702/1‑49 (MQ=16) atgatCTTCAGGAGCTGGAACTGAAGGGCGGTGAAACGATCCTCGCGAAGCTGGTGATTGGTGCCGACg > 1:3262903/1‑69 (MQ=255) atgatCTTCAGGAGCTGGAACTGAAGGGCGGTGAAACGATCCTCGCGAAGCTGGTGATTGGTGCCGACg > 1:795538/1‑69 (MQ=255) atgatCTTCAGGAGCTGGAACTGAAGGGCGGTGAAACGATCCTCGCGAAGCTGGTGATTGGTGCCGACg > 1:2897647/1‑69 (MQ=255) atgatCTTCAGGAGCTGGAACTGAAGGGCGGTGAAACGATCCTCGCGAAGCTGGTGATTGGTGCCGACg > 1:369775/1‑69 (MQ=255) atgatCTTCAGGAGCTGGAACTGAAGGGCGGTGAAACGATCCTCGCGAAGCTGGTGATTGGTGCCGACg > 1:2149064/1‑69 (MQ=255) atgatCTTCAGGAGCTGGAACTGAAGGGCGGTGAAACGATCCTCGCGAAGCTGGTGATTGGTGCCGACg > 1:663447/1‑69 (MQ=255) atgatCTTCAGGAGCTGGAACTGAAGGGCGGTGAAACGATACTCGCGAAGCTGGTGATTGGTGCCGAc > 1:3242918/1‑68 (MQ=255) atCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGgc > 1:2771690/1‑68 (MQ=255) cTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGt < 1:796284/50‑1 (MQ=255) ttCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGt < 1:19978/49‑1 (MQ=255) ggagctggagctGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCa < 1:2032465/64‑1 (MQ=255) ggagctggagctGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCa < 1:2594330/64‑1 (MQ=255) ggagctggagctGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCa < 1:1279733/64‑1 (MQ=255) ggagctggagctGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCa < 1:1758647/64‑1 (MQ=255) ggagctggagctGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCa < 1:1189371/64‑1 (MQ=255) ggagctggagctGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCa < 1:2442349/64‑1 (MQ=255) ggagctggagctGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAAtt < 1:1688532/68‑1 (MQ=255) gctggagctgAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGc > 1:1487622/1‑68 (MQ=255) gctggagctgAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCa > 1:419949/1‑69 (MQ=255) gctggagctgAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCa > 1:1247551/1‑69 (MQ=255) gctggagctgAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCa > 1:631879/1‑69 (MQ=255) gctggagctgAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCa > 1:1450999/1‑69 (MQ=255) gctggagctgAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCa > 1:784420/1‑69 (MQ=255) gctggagctgAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCa > 1:3356539/1‑69 (MQ=255) gctggagctgAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCa > 1:1605574/1‑69 (MQ=255) gctcgagctgAAAGGCGGAGAAGTGATTCGCGCGAAGCTGGTGATTCGAGCCGACgg > 1:1422457/1‑57 (MQ=255) | GTGTGCCAGGCTCGCTGATTGCGCTGCATCGCCATGATGATCTTCAGGAGCTGGAGCTGAAAGGCGGTGAAGTGATTCGCGCGAAGCTGGTGATTGGTGCCGACGGCGCAAATTCGCA > minE/418793‑418910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |