| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 50,330 | A→G | 50.4% | L255L (CTT→CTC) | pdxA ← | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 50,330 | 0 | A | G | 50.4% | 0.9 / 129.3 | 139 | L255L (CTT→CTC) | pdxA | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (49/20); new base G (51/19); total (100/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 139 total aligned reads displayed.
TTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGCGCC > minE/50273‑50393 | ttttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGa > 1:905552/1‑61 (MQ=255)ttttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGa > 1:74212/1‑61 (MQ=255)ttttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGa > 1:642562/1‑61 (MQ=255)ttttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGa > 1:35836/1‑61 (MQ=255)ttttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGa > 1:3418937/1‑61 (MQ=255)ttttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGa > 1:3349743/1‑61 (MQ=255)ttttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGa > 1:2617162/1‑61 (MQ=255)ttttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGa > 1:2058947/1‑61 (MQ=255)ttttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGa > 1:1862165/1‑61 (MQ=255)ttttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGa > 1:153301/1‑61 (MQ=255)ttttAGCACGGGAAGACCCTGATAGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGa > 1:1705891/1‑61 (MQ=255) ttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTc > 1:809430/1‑65 (MQ=255) ttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGc > 1:1050984/1‑68 (MQ=255) ttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGc > 1:810765/1‑68 (MQ=255) ttAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGc > 1:2870571/1‑68 (MQ=255) aCGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGaaa < 1:1799287/68‑1 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:1527779/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:1923973/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:1899215/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:1972563/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:1993642/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:1858691/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:2064289/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:2377664/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:2867800/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:1276925/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:3206829/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:3361741/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:53732/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:594075/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:1576686/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:661405/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGa > 1:1388637/1‑58 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATc > 1:1713697/1‑69 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATc > 1:34681/1‑69 (MQ=255) aCCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATc > 1:1609502/1‑69 (MQ=255) aTCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAgg > 1:2536532/1‑58 (MQ=255) aTCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAgg > 1:2262171/1‑58 (MQ=255) aTCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAgg > 1:1041541/1‑58 (MQ=255) aTCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCggca > 1:2566224/1‑67 (MQ=255) aTCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCggca > 1:1287265/1‑67 (MQ=255) gTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCgg > 1:3015680/1‑69 (MQ=255) gTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCgg > 1:2625654/1‑69 (MQ=255) gTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCgg > 1:2009816/1‑69 (MQ=255) gTACATCGCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCgg > 1:2280499/1‑69 (MQ=255) aCATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGcc < 1:1954553/69‑1 (MQ=255) aCATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGcc < 1:3052293/69‑1 (MQ=255) aCATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGcc < 1:3358079/69‑1 (MQ=255) aCATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGcc < 1:2175216/69‑1 (MQ=255) aCATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGcc < 1:862610/69‑1 (MQ=255) cATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGccc > 1:1746190/1‑69 (MQ=255) cATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGccc > 1:1091492/1‑69 (MQ=255) cATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGccc > 1:3298464/1‑69 (MQ=255) cATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGccc > 1:3219148/1‑69 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACa > 1:1552085/1‑43 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACa > 1:1636084/1‑43 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACa > 1:378559/1‑43 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACa > 1:1151711/1‑43 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACa > 1:1191233/1‑43 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACa > 1:1056225/1‑43 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACa > 1:195329/1‑43 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACa > 1:1357386/1‑43 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACa > 1:1880138/1‑43 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACa > 1:221845/1‑43 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACa > 1:2236624/1‑43 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACa > 1:133263/1‑43 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACa > 1:2258503/1‑43 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:1098868/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:161921/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:2037926/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:175652/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:1642294/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:1217597/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:3301261/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:1279812/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:3220185/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:1684808/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:2937848/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:3054790/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:3046570/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg < 1:2743249/69‑1 (MQ=255) gCCAGCACGGCGTCGGCGTTATCGAGATATTTCGGCTGAAACAGGGTATAGGCAGGCAGCGGCCCGTTg < 1:3406222/69‑1 (MQ=255) aGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGt < 1:2892282/69‑1 (MQ=255) aGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGt < 1:53103/69‑1 (MQ=255) gCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGt < 1:269298/68‑1 (MQ=255) aCGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTc < 1:2667063/69‑1 (MQ=255) aCGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTc < 1:702942/69‑1 (MQ=255) aCGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTc < 1:809098/69‑1 (MQ=255) aCGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTc < 1:641301/69‑1 (MQ=255) aCGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTc < 1:3117370/69‑1 (MQ=255) aCGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTc < 1:3179235/69‑1 (MQ=255) aCGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTc < 1:1668237/69‑1 (MQ=255) aCGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTc < 1:1734417/69‑1 (MQ=255) aTCAAGATATTTCGGCTGAAACAGGGTATCggcaggc > 1:1725066/1‑37 (MQ=255) aTCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTg > 1:2514850/1‑49 (MQ=255) aTCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGCGcc > 1:1136100/1‑67 (MQ=255) aTCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGCGcc > 1:1795955/1‑67 (MQ=255) aTCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGCGcc > 1:496923/1‑67 (MQ=255) aTCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGCGcc > 1:476340/1‑67 (MQ=255) aTCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGCGcc > 1:1352274/1‑67 (MQ=255) | TTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCGGCGTTATCAAGATATTTCGGCTGAAACAGGGTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGCGCC > minE/50273‑50393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |