| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 423,948 | C→T | 44.4% | E316E (GAG→GAA) | nagC ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 423,948 | 0 | C | T | 44.4% | 10.3 / 40.7 | 54 | E316E (GAG→GAA) | nagC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (23/7); new base T (13/11); total (36/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.29e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
GCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTC > minE/423890‑424016 | gCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACt < 1:2546939/68‑1 (MQ=255)gCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACt < 1:1589776/68‑1 (MQ=255) ttAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATATTCAAtc < 1:1224392/60‑2 (MQ=255) ttAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATATTCAAtc < 1:127276/60‑2 (MQ=255) ttAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATATTCAAtc < 1:2140068/60‑2 (MQ=255) ttAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATATTCAAtc < 1:359384/60‑2 (MQ=255) ttAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATATTCAAtc < 1:2841774/60‑2 (MQ=255) ttAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATATTCAAtc < 1:2722717/60‑2 (MQ=255) ttAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATATTCAAtc < 1:3150095/60‑2 (MQ=255) ttAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATATTCAAtc < 1:2992403/60‑2 (MQ=255) ttAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATATTCAAtc < 1:1873873/60‑2 (MQ=255) ttAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATATTCAAtc < 1:3090353/60‑2 (MQ=255) tAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATATTCAAtc < 1:3092996/59‑2 (MQ=255) gCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGcc > 1:2257439/1‑68 (MQ=255) gCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCt > 1:2375872/1‑69 (MQ=255) gCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCt > 1:2831701/1‑69 (MQ=255) gCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCt > 1:3310372/1‑69 (MQ=255) gCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCt > 1:2639576/1‑69 (MQ=255) aTGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTg > 1:2040861/1‑69 (MQ=255) aTGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTg > 1:611437/1‑69 (MQ=255) gagggTTTTACCCAGATGACGACCGACATATTCAATCACTTCCGAGGCCAGGCTATCGCCTTTGTTcg > 1:1776778/4‑68 (MQ=255) gATGGTTTTACCCAGATGACGACCGACATATTCAATCACTTCCGAGGCCAGGCTATCGCCTTTGTTcgc > 1:89124/1‑69 (MQ=255) gATGGTTTTACCCAGATGACGACCGACATATTCAATCACTTCCGAGGCCAGGCTATCGCCTTTGTTcgc > 1:2747669/1‑69 (MQ=255) gATGGTTTTACCCAGATGACGACCGACATATTCAATCACTTCCGAGGCCAGGCTATCGCCTTTGTTcgc > 1:2632527/1‑69 (MQ=255) gATGGTTTTACCCAGATGACGACCGACATATTCAATCACTTCCGAGGCCAGGCTATCGCCTTTGTTcgc > 1:2748706/1‑69 (MQ=255) gATGGTTTTACCCAGATGACGACCGACATATTCAATCACTTCCGAGGCCAGGCTATCGCCTTTGTTcgc > 1:279974/1‑69 (MQ=255) gATGGTTTTACCCAGATGACGACCGACATATTCAATCACTTCCGAGGCCAGGCTATCGCCTTTGTTcgc > 1:2831648/1‑69 (MQ=255) gATGGTTTTACCCAGATGACGACCGACATATTCAATCACTTCCGAGGCCAGGCTATCGCCTTTGTTcgc > 1:3256724/1‑69 (MQ=255) gATGGTTTTACCCAGATGACGACCGACATATTCAATCACTTCCGAGGCCAGGCTATCGCCTTTGTTcgc > 1:3260802/1‑69 (MQ=255) gATGGTTTTACCCAGATGACGACCGACATATTCAATCACTTCCGAGGCCAGGCTATCGCCTTTGTTcgc > 1:444162/1‑69 (MQ=255) gATGGTTTTACCCAGATGACGACCGACATATTCAATCACTTCCGAGGCCAGGCTATCGCCTTTGTTcgc > 1:713633/1‑69 (MQ=255) gATGGTTTTACCCAGATGACGACCGACATATTCAATCACTTCCGAGGCCAGGCTATCGCCTTTGTTcgc > 1:2583271/1‑69 (MQ=255) gATGGTTTTACCCAGATGACGACCGACATATTCAATCACTTCCGAGGCCAGGCTATCGCCTTTGTTcg > 1:120051/1‑68 (MQ=255) tGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCgg > 1:3352662/1‑69 (MQ=255) tGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCgg > 1:250918/1‑69 (MQ=255) tGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCgg > 1:2264083/1‑69 (MQ=255) ttACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCgg > 1:768015/1‑64 (MQ=255) tGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtt > 1:2987232/1‑69 (MQ=255) gacgacCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtt > 1:14394/1‑68 (MQ=255) gacgacCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtt > 1:1354006/1‑68 (MQ=255) gacgacCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtt > 1:1248505/1‑68 (MQ=255) acgacCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:2835399/69‑1 (MQ=255) acgacCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:2321829/69‑1 (MQ=255) acgacCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:2324239/69‑1 (MQ=255) acgacCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGtttt < 1:2513664/69‑1 (MQ=255) accgacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGAt > 1:852223/1‑69 (MQ=255) accgacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGAt > 1:804161/1‑69 (MQ=255) accgacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGAt > 1:2615373/1‑69 (MQ=255) accgacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGAt > 1:2764638/1‑69 (MQ=255) accgacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGAt > 1:470054/1‑69 (MQ=255) accgacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGAt > 1:3432243/1‑69 (MQ=255) accgacATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGAt > 1:2662999/1‑69 (MQ=255) cATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCgg < 1:228784/45‑1 (MQ=255) aTACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTAGATGGTGCa > 1:1135707/1‑69 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGtt > 1:33088/1‑36 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGc > 1:1449464/1‑42 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGc > 1:345287/1‑42 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGc < 1:1496774/42‑1 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGc < 1:1745862/42‑1 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGc > 1:628983/1‑42 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGc > 1:1929542/1‑42 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGc > 1:2845229/1‑42 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGc > 1:2129673/1‑42 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGc > 1:2814588/1‑42 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgtc > 1:3312162/1‑69 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgtc > 1:166855/1‑69 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgtc > 1:1917738/1‑69 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgt > 1:1360210/1‑68 (MQ=255) cTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgt > 1:576326/1‑68 (MQ=255) | GCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTC > minE/423890‑424016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |