| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 424,830 | C→T | 32.4% | A22A (GCG→GCA) | nagC ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 424,830 | 0 | C | T | 32.4% | 68.7 / 38.7 | 80 | A22A (GCG→GCA) | nagC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (18/36); new base T (17/9); total (35/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.69e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGT > minE/424767‑424890 | cTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACcgccgc > 1:2926748/1‑69 (MQ=255) cTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGtttt > 1:279063/1‑67 (MQ=255) cTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGtttt > 1:3295128/1‑67 (MQ=255) cTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGtttt > 1:260305/1‑67 (MQ=255) cTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGtttt > 1:20816/1‑67 (MQ=255) tGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGt > 1:2206643/1‑63 (MQ=255) tGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGt > 1:3296627/1‑63 (MQ=255) tGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGt > 1:3429040/1‑63 (MQ=255) tGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGt > 1:676566/1‑63 (MQ=255) tCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACgag < 1:590665/67‑1 (MQ=255) tCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACgag < 1:2556339/67‑1 (MQ=255) tCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACgag < 1:2878428/67‑1 (MQ=255) cgAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAAc < 1:480979/69‑1 (MQ=255) cgAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAAc < 1:2614387/69‑1 (MQ=255) cgAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAAc < 1:3301784/69‑1 (MQ=255) cgAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAAc < 1:2069733/69‑1 (MQ=255) cgAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAAc < 1:1219114/69‑1 (MQ=255) aTTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAAc < 1:1170969/65‑1 (MQ=255) gTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTg < 1:228671/69‑1 (MQ=255) gTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTg < 1:3364670/69‑1 (MQ=255) gTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTg < 1:1516375/69‑1 (MQ=255) gTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTg < 1:1359001/69‑1 (MQ=255) gTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTg < 1:66533/69‑1 (MQ=255) gTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACATATCTg < 1:1839602/69‑1 (MQ=255) gTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTAAGAGATCAACATTACCTATCTg < 1:329807/69‑1 (MQ=255) gTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg > 1:285235/1‑69 (MQ=255) gTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg > 1:2178192/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg > 1:2914407/1‑68 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg > 1:87277/1‑68 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg > 1:2221477/1‑68 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:2431202/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:2491485/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:2435107/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:2765254/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:297072/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:2921654/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:1173070/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:1219157/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:1723251/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:1364556/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:2025623/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:1673475/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:204214/1‑69 (MQ=255) tCAATCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGt > 1:1685897/1‑69 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:1063089/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:2964254/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:3054644/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:3087291/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:3281387/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:1571241/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:1494479/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:1379257/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:664878/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:682691/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:944584/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:2194731/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:1980051/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:874483/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:2094303/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:2608534/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:26758/67‑1 (MQ=255) cAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTc > 1:1887513/1‑69 (MQ=255) aaTCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:2262973/66‑1 (MQ=255) aaTCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTg < 1:2648681/66‑1 (MQ=255) tCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGc < 1:2119319/69‑1 (MQ=255) tCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGc < 1:154252/69‑1 (MQ=255) tCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGc < 1:3410324/69‑1 (MQ=255) tCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGc < 1:68752/69‑1 (MQ=255) tCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGc < 1:191788/69‑1 (MQ=255) tCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGc < 1:2195691/69‑1 (MQ=255) tCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGc < 1:1914000/69‑1 (MQ=255) tCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGc < 1:2510398/69‑1 (MQ=255) tCAGGCGATAAACTGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATAACCTATCTGAGCTTGTCCGc < 1:1681080/69‑1 (MQ=255) gCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTg < 1:1648638/68‑1 (MQ=255) cGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGgt > 1:972958/1‑69 (MQ=255) cGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGgt > 1:3245602/1‑69 (MQ=255) cGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGgt > 1:2288046/1‑69 (MQ=255) gATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGgt > 1:3252413/1‑68 (MQ=255) gATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGgt > 1:2845929/1‑68 (MQ=255) gATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGgt > 1:887040/1‑68 (MQ=255) gATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGgt > 1:2540840/1‑68 (MQ=255) | CTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGT > minE/424767‑424890 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |