| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 50,516 | C→G | 38.2% | R193R (CGG→CGC) | pdxA ← | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 50,516 | 0 | C | G | 38.2% | 122.1 / 153.9 | 165 | R193R (CGG→CGC) | pdxA | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (63/39); new base G (31/32); total (94/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 165 total aligned reads displayed.
GCCTTCGCCCGCGTGCGGATTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCGTCTGCGA > minE/50450‑50580 | gCCTTCGCCCGCGTGCGGATTCAGCCCGCAGACCATAATGCGCGGTTCGGCAATACCAAATTTGGTCCg < 1:2675904/69‑1 (MQ=255) ccGCGTGCGGATTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGt < 1:1332105/69‑1 (MQ=255) ccGCGTGCGGATTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGt < 1:2070542/69‑1 (MQ=255) cgcgTGCGGATTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTg < 1:2299563/69‑1 (MQ=255) cgcgTGCGGATTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTg < 1:1698043/69‑1 (MQ=255) cgcgTGCGGATTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTg > 1:3186865/1‑69 (MQ=255) cgcgTGCGGATTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTg > 1:1393574/1‑69 (MQ=255) cgcgTGCGGATTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTg > 1:2983409/1‑69 (MQ=255) cgcgTGCGGATTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTg > 1:1226086/1‑69 (MQ=255) cgcgTGCGGATTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTg < 1:2800440/69‑1 (MQ=255) cgcgTGCGGATTCAGACCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTg > 1:2753377/1‑69 (MQ=255) cgTGCGGATTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTg < 1:2192100/67‑1 (MQ=255) gATTCAGCCCGCAGACCAGAAT‑‑GCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGTGATGCAAAATa < 1:1407416/69‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGt < 1:2386924/59‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGt < 1:30598/59‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGt < 1:310558/59‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGt < 1:3119499/59‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGt < 1:230425/59‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACAAAATTTGGTGCGCAAATCGt < 1:866039/59‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACAAAATTTGGTGCGCAAATCGt < 1:1624307/59‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACAAAATTTGGTGCGCAAATCGt < 1:1683439/59‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACAAAATTTGGTGCGCAAATCGt < 1:169292/59‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACAAAATTTGGTGCGCAAATCGt < 1:714778/59‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACAAAATTTGGTGCGCAAATCGt < 1:2885999/59‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACAAAATTTGGTGCGCAAATCGt < 1:1266800/59‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACAAAATTTGGTGCGCAAATCGt < 1:2038143/59‑1 (MQ=255) aTTCAGCCCGCAGACCAGAATCCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGt < 1:195853/59‑1 (MQ=255) ttCAGCCTGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGTGATGCAAAATa < 1:1310884/69‑1 (MQ=255) ttCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGt < 1:323886/58‑1 (MQ=255) ttCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGTGATGCAAAATa < 1:1472834/69‑1 (MQ=255) ttCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGTGATGCAAAATa < 1:482095/69‑1 (MQ=255) ttCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGTGATGCAAAATa < 1:2843703/69‑1 (MQ=255) ttCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGTGATGCAAAATa < 1:154768/69‑1 (MQ=255) ttCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACAAAATTTGGTGCGCAAATCGt < 1:1415151/58‑1 (MQ=255) gCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCa > 1:3321625/1‑68 (MQ=255) cccGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGc < 1:1536709/66‑1 (MQ=255) cccGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGc < 1:1898216/66‑1 (MQ=255) cccGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGc < 1:2454615/66‑1 (MQ=255) cccGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGc < 1:2801694/66‑1 (MQ=255) cccGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGc < 1:2334469/66‑1 (MQ=255) gACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTc > 1:1872678/1‑69 (MQ=255) gACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTc > 1:973726/1‑69 (MQ=255) gACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTc > 1:1520193/1‑69 (MQ=255) gACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTc > 1:704059/1‑69 (MQ=255) aTACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCa > 1:248357/1‑60 (MQ=255) aTACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCa > 1:705049/1‑60 (MQ=255) aTACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCa > 1:632204/1‑60 (MQ=255) aTACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCa > 1:2251544/1‑60 (MQ=255) aTACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCa > 1:2239778/1‑60 (MQ=255) aTACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCa > 1:853656/1‑60 (MQ=255) aTACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCa > 1:286528/1‑60 (MQ=255) aTACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCa > 1:3225545/1‑60 (MQ=255) aTACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCa > 1:3265870/1‑60 (MQ=255) aTACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCa > 1:1243897/1‑60 (MQ=255) aTACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCa > 1:406583/1‑60 (MQ=255) aCCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGt < 1:466237/63‑1 (MQ=255) aCCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGt < 1:1107870/63‑1 (MQ=255) aCCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGt < 1:822816/63‑1 (MQ=255) aCCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGt < 1:1072100/63‑1 (MQ=255) aCCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGt < 1:2078977/63‑1 (MQ=255) aCCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAg > 1:1139716/1‑68 (MQ=255) aCCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAg > 1:2029604/1‑68 (MQ=255) aCCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGc > 1:2376371/1‑69 (MQ=255) aCCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGc > 1:587560/1‑69 (MQ=255) aCCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGc > 1:2009269/1‑69 (MQ=255) aCCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGc > 1:538255/1‑69 (MQ=255) aCCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGc > 1:2441570/1‑69 (MQ=255) aCCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGc > 1:920140/1‑69 (MQ=255) ccAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGc > 1:1311998/1‑68 (MQ=255) ccAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCg > 1:191649/1‑69 (MQ=255) ccAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCg > 1:1218283/1‑69 (MQ=255) ccAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCg > 1:15947/1‑69 (MQ=255) ccAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCg > 1:1797400/1‑69 (MQ=255) ccAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCg > 1:2138713/1‑69 (MQ=255) ccAAATCTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCg > 1:940821/1‑69 (MQ=255) ccAAATCTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCg > 1:383437/1‑69 (MQ=255) aaaTTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCGTc > 1:2094086/1‑69 (MQ=255) aaaTTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCGTc > 1:2126708/1‑69 (MQ=255) tttGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCGTCTGc < 1:991850/69‑1 (MQ=255) tttGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCGTCTGc < 1:3417698/69‑1 (MQ=255) tttGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCGTCTGc < 1:1485671/69‑1 (MQ=255) tttGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCGTCTGc < 1:2188228/69‑1 (MQ=255) tttGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCGTCTGc < 1:2407743/69‑1 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAgg > 1:736535/1‑46 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:2206937/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:1182785/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:456879/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:970900/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:1858145/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:1914097/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:2102859/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:334915/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:823847/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:309215/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:518966/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:2593615/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:63336/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:2647887/1‑69 (MQ=255) tGGTGCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGGGCAGGGGTGATAGCATCTGCGa > 1:2764041/1‑69 (MQ=255) tGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCGTCTGCGa < 1:2571466/69‑1 (MQ=255) | GCCTTCGCCCGCGTGCGGATTCAGCCCGCAGACCAGAATGCGCGGTTCGGCAATACCAAATTTGGTCCGCAAATCGTGATGCAAAATAGCAATCACTTCGTGCAAAAGTGCAGGGGTGATAGCGTCTGCGA > minE/50450‑50580 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |