| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 425,081 | G→A | 60.6% | C324C (TGC→TGT) | nagA ← | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 425,081 | 0 | G | A | 60.6% | 28.0 / 43.4 | 66 | C324C (TGC→TGT) | nagA | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Reads supporting (aligned to +/- strand): ref base G (22/4); new base A (25/15); total (47/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.35e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTT > minE/425014‑425146 | tctcAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGc > 1:2662686/1‑69 (MQ=255)tctcAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGc > 1:600556/1‑69 (MQ=255)tctcAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGc > 1:2670625/1‑69 (MQ=255)tctcAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGc > 1:2793548/1‑69 (MQ=255)tctcAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGc > 1:1539249/1‑69 (MQ=255)tctcAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGc > 1:3217111/1‑69 (MQ=255) tCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCa < 1:2319133/69‑1 (MQ=255) cGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:83533/1‑69 (MQ=255) cGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:3241958/1‑69 (MQ=255) cGTAGCACTTCATCCAGAGCGATACCGCAATGTTCGACCAGATTACGCGCGCCTTCAATCATGGTTaa > 1:3023461/1‑68 (MQ=255) gCACTTCATCGAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:3082088/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:3331825/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:3119637/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:3038038/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:3388134/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:2876797/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:1851040/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:1334636/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:2750953/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:567470/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:2134267/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:854605/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:2522625/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATg < 1:906268/69‑1 (MQ=255) gCACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGAACGCCTTCAATCATGGTTAAGGATg < 1:254418/69‑1 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGCTTACGCACGCCTTCAATCATGGTTAAg > 1:1059954/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTacgctc > 1:2192020/1‑43 (MQ=38) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGGCTTCAATCATGGTTAAg > 1:1735871/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGt > 1:115279/1‑60 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:1437689/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:960786/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:105031/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:802624/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:53472/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:411799/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:3409918/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:1410669/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:1413497/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:2320347/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:16756/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:1691086/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:1818816/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:2418755/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:1857121/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:2421706/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:2516016/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:1978365/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:2271043/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAg > 1:2146735/1‑64 (MQ=255) cACTTCATCCAGTGCGATACCACAATGTTCGACCAGATTACGCACGCCTTCAATAATGGTTAAg > 1:193450/1‑64 (MQ=255) aCTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCAGGCCTTCAATCATGGTTAAGGATGa < 1:3363449/68‑1 (MQ=255) aCTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGa < 1:318724/68‑1 (MQ=255) tgTGCGATACCGCATTGTTCGACCTGATTACGCACGGCTTCAATCATGGTTAAGGATGa < 1:2791104/58‑1 (MQ=255) cGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTAc > 1:608554/1‑69 (MQ=255) cGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTAc > 1:1278904/1‑69 (MQ=255) aCCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGtt > 1:703954/1‑69 (MQ=255) aCCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGtt > 1:2322422/1‑69 (MQ=255) aCCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGtt > 1:66228/1‑69 (MQ=255) aCCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGtt > 1:1690625/1‑69 (MQ=255) aCCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGt > 1:2976529/1‑68 (MQ=255) ccGCAATGTTCGACCAGATTACGCACGCCTTCAAt > 1:1260740/1‑35 (MQ=255) ccGCAATGTTCGACCAGATTACGCACGCCTTCAAt > 1:3422715/1‑35 (MQ=255) ccGCAATGTTCGACCAGATTACGCACGCCTTCAAt > 1:3391198/1‑35 (MQ=255) ccGCAATGTTCGACCAGATTACGCACGCCTTCAAt > 1:1230270/1‑35 (MQ=255) ccGCAATGTTCGACCAGATTACGCACGCCTTCAAt > 1:3325210/1‑35 (MQ=255) ccGCAATGTTCGACCAGATTACGCACGCCTTCAAt > 1:1504696/1‑35 (MQ=255) | TCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTT > minE/425014‑425146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |