| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 428,771 | C→T | 31.5% | V509V (GTC→GTT) | nagE → | fused N‑acetyl glucosamine specific PTS enzyme IICBA components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 428,771 | 0 | C | T | 31.5% | 90.6 / 48.0 | 98 | V509V (GTC→GTT) | nagE | fused N‑acetyl glucosamine specific PTS enzyme IICBA components |
| Reads supporting (aligned to +/- strand): ref base C (34/33); new base T (8/23); total (42/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.79e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAACCGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGA > minE/428705‑428838 | aaaaCCGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGt > 1:3411861/1‑69 (MQ=255) aaCCGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTgg < 1:1195414/69‑1 (MQ=255) aCCGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGc < 1:1639662/69‑1 (MQ=255) aCCGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGc < 1:1406108/69‑1 (MQ=255) ccGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGGGATGTTGTGGCa > 1:69461/1‑69 (MQ=255) ccGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGGGATGTTGTGGCa > 1:2028288/1‑69 (MQ=255) ccGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGGGATGTTGTGGCa > 1:1844493/1‑69 (MQ=255) ccGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGGGATGTTGTGGCa > 1:3090227/1‑69 (MQ=255) ccGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGGGATGTTGTGGCa > 1:2573490/1‑69 (MQ=255) ccGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGGGATGTTGTGGCa > 1:3076864/1‑69 (MQ=255) ccGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGGGATGTTGTGGCa > 1:1472407/1‑69 (MQ=255) ccGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGGGATGTTGTGGCa > 1:1424699/1‑69 (MQ=255) gTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGaa < 1:1651306/69‑1 (MQ=255) gTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGaa < 1:2623519/69‑1 (MQ=255) tatctatcGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAg < 1:3428908/69‑1 (MQ=255) tatctatcGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAg < 1:1743406/69‑1 (MQ=255) atctatcGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGc > 1:80604/1‑69 (MQ=255) atctatcGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGc > 1:1822081/1‑69 (MQ=255) tctatcGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCa > 1:3039154/1‑69 (MQ=255) tatcGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCAtt > 1:566748/1‑69 (MQ=255) tatcGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCAtt > 1:1999442/1‑69 (MQ=255) tatcGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCAtt > 1:1117814/1‑69 (MQ=255) tatcGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCAtt > 1:2534061/1‑69 (MQ=255) gCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCaa > 1:3230006/1‑69 (MQ=255) gCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCaa > 1:2878167/1‑69 (MQ=255) gCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCaa > 1:1511876/1‑69 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:600832/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:3038573/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:638441/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:2476656/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:2706012/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:2755058/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:235328/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:2962683/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:2264477/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:3025982/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:3373512/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:768865/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:1468954/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:148809/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:1261503/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:1542653/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:838004/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:1838843/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:1260127/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:1788817/69‑1 (MQ=255) ggTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:1780394/69‑1 (MQ=255) gTATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:2004967/68‑1 (MQ=255) tATCGCCGATTACCGGGGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGc < 1:2722614/67‑1 (MQ=255) aTCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggt > 1:889947/1‑69 (MQ=255) aTCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggt > 1:508937/1‑69 (MQ=255) aTCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggt > 1:327067/1‑69 (MQ=255) aTCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggt > 1:3158763/1‑69 (MQ=255) aTCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggt > 1:2073616/1‑69 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGTATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:2471867/69‑1 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtt < 1:2218578/69‑2 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:421912/69‑1 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:3204419/69‑1 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg > 1:1011402/1‑69 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:3329779/69‑1 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:3216397/69‑1 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:3097304/69‑1 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:3163833/69‑1 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:2800521/69‑1 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:1389707/69‑1 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:2653793/69‑1 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:254666/69‑1 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:1437467/69‑1 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:1821697/69‑1 (MQ=255) tCGCCGATTACCGGTGATGTCGTGGCACGGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:3122168/69‑1 (MQ=255) gCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAg < 1:3023591/47‑1 (MQ=255) gCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAg < 1:1496322/47‑1 (MQ=255) gCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAg < 1:1773882/47‑1 (MQ=255) gCCGATTACCGGTGATGTCGTGGCACTGGATCAAGTTCCTGACGAAg < 1:2360906/47‑1 (MQ=255) gCCGATTACCGGGGATGTCGTGGCACTGGATCAGGTTCCTGACGAAg < 1:469441/47‑1 (MQ=255) tACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:3429246/61‑1 (MQ=255) tACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACgg < 1:2296458/69‑1 (MQ=255) tACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACgg > 1:2683384/1‑69 (MQ=255) tACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACgg > 1:1277492/1‑69 (MQ=255) tACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACgg > 1:2761731/1‑69 (MQ=255) aTGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtg < 1:2185440/53‑1 (MQ=255) aTGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCg > 1:874842/1‑68 (MQ=255) aTGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCg > 1:702165/1‑68 (MQ=255) aTGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCg > 1:984030/1‑68 (MQ=255) aTGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCg > 1:495362/1‑68 (MQ=255) aTGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCg > 1:308349/1‑68 (MQ=255) aTGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCg > 1:2204574/1‑68 (MQ=255) aTGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCg > 1:2108090/1‑68 (MQ=255) aTGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCg > 1:1366213/1‑68 (MQ=255) tCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGa > 1:52428/1‑69 (MQ=255) tCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGa > 1:2894148/1‑69 (MQ=255) tCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGa < 1:2798970/69‑1 (MQ=255) tCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGa < 1:251283/69‑1 (MQ=255) tCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGa > 1:2125819/1‑69 (MQ=255) tCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGa > 1:1971772/1‑69 (MQ=255) tCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGa > 1:1952872/1‑69 (MQ=255) tCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGa < 1:1804227/69‑1 (MQ=255) tCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGa > 1:1095392/1‑69 (MQ=255) | AAAACCGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGA > minE/428705‑428838 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |