Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 434,130 | C→T | 39.9% | intergenic (‑183/+106) | fur ← / ← fldA | DNA‑binding transcriptional dual regulator/flavodoxin 1 |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 434,130 | 0 | C | T | 39.9% | 40.7 / 56.8 | 85 | intergenic (‑183/+106) | fur/fldA | DNA‑binding transcriptional dual regulator/flavodoxin 1 |
Reads supporting (aligned to +/- strand): ref base C (32/19); new base T (27/7); total (59/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.49e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01 |
GTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCT > minE/434076‑434197 | gTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACa < 1:2468372/68‑1 (MQ=255) gTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACa < 1:2214577/68‑1 (MQ=255) gTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACa < 1:282094/68‑1 (MQ=255) gTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACa < 1:494295/68‑1 (MQ=255) gTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACa < 1:3263533/68‑1 (MQ=255) gTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACa < 1:2236482/68‑1 (MQ=255) gTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACa < 1:3346722/68‑1 (MQ=255) ttAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAgg > 1:2740109/1‑69 (MQ=255) taaAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTac < 1:3124471/68‑1 (MQ=255) aaaaCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTacaac > 1:2184213/1‑69 (MQ=255) aaaaCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTacaac > 1:1842201/1‑69 (MQ=255) aaaaCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTacaac > 1:1291450/1‑69 (MQ=255) aaaaCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTacaac > 1:2045774/1‑69 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGGACACTGGGACATTGTACAGGTacaaca > 1:452810/1‑67 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTacaaca > 1:531479/1‑67 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTacaaca > 1:2203909/1‑67 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTacaaca > 1:157536/1‑67 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTacaaca > 1:1638946/1‑67 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTacaac > 1:3175354/1‑66 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:2246972/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:2217621/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:2933749/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:2631256/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:2749722/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:1036248/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:3368514/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:40389/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:479299/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:596565/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:652983/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:764707/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:845851/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:1352322/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:1026672/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:2029889/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:1369616/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:106595/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:1113306/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:1389152/1‑68 (MQ=255) aCGTGGCACAGCTTACATTTACAACAGCAAGGCCACTTGAACACTGGGACATTGTACAGGTACAACAg > 1:1162594/1‑68 (MQ=255) ggCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTg < 1:1328794/50‑1 (MQ=255) gCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:908815/1‑69 (MQ=255) gCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:827234/1‑69 (MQ=255) gCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:242614/1‑69 (MQ=255) gCTTACATTTACAACGGAAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:2151158/1‑69 (MQ=255) cAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGc < 1:3043447/68‑1 (MQ=255) ggCCACTTGAACACTGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:570183/69‑1 (MQ=255) ggCCACTTGAACACTGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:1234045/69‑1 (MQ=255) ggCCACTTGAACACTGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:378794/69‑1 (MQ=255) ggCCACTTGAACACTGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:132372/69‑1 (MQ=255) ggCCACTTGAACACTGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:1673028/69‑1 (MQ=255) ggCCACTTGAACACTGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:596192/69‑1 (MQ=255) ggCCACTTGAACACTGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:2822994/69‑1 (MQ=255) gCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa > 1:788724/1‑69 (MQ=255) gCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa > 1:83191/1‑69 (MQ=255) gCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa > 1:1343109/1‑69 (MQ=255) gCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa > 1:2137597/1‑69 (MQ=255) gCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa > 1:3051700/1‑69 (MQ=255) gCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa > 1:2131763/1‑69 (MQ=255) gCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa > 1:2101207/1‑69 (MQ=255) gCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa > 1:2404274/1‑69 (MQ=255) gCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa > 1:1833965/1‑69 (MQ=255) gCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa > 1:2532479/1‑69 (MQ=255) cACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGAtt > 1:1349450/1‑69 (MQ=255) cACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGAtt > 1:1147537/1‑69 (MQ=255) cTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTgg < 1:3056949/69‑1 (MQ=255) cTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTgg < 1:2487495/69‑1 (MQ=255) tGAACACCGGGACATTGTACAGGTACCACAGCAAAAGTTACa > 1:2881541/1‑42 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:545367/1‑42 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:2260065/1‑42 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:1364255/1‑42 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:1075247/1‑42 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:2441031/1‑42 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:3293677/1‑42 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:505183/1‑42 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:1233283/1‑42 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:2707700/1‑42 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:2711561/1‑42 (MQ=255) acacCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCAt < 1:1151402/67‑1 (MQ=255) acacCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCAt < 1:762421/67‑1 (MQ=255) acacCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCAt < 1:44214/67‑1 (MQ=255) acacCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCAt < 1:1791394/67‑1 (MQ=255) acacCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCAt < 1:1943389/67‑1 (MQ=255) cacCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCAt < 1:530159/66‑1 (MQ=255) cacCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCAt < 1:488832/66‑1 (MQ=255) ccGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCt > 1:3297197/1‑69 (MQ=255) ccGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCt > 1:2286478/1‑69 (MQ=255) | GTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCT > minE/434076‑434197 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |