| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 51,307 | A→G | 36.5% | T358T (ACT→ACC) | surA ← | peptidyl‑prolyl cis‑trans isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 51,307 | 0 | A | G | 36.5% | 27.6 / 30.3 | 52 | T358T (ACT→ACC) | surA | peptidyl‑prolyl cis‑trans isomerase |
| Reads supporting (aligned to +/- strand): ref base A (10/23); new base G (6/13); total (16/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTAAATGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCGCCGCC > minE/51248‑51369 | aTTTAATGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCACGGc < 1:346165/68‑1 (MQ=255)aTTAAATGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGc < 1:3176606/68‑1 (MQ=255)aTTAAATGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGc < 1:2249307/68‑1 (MQ=255)aTTAAATGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGc < 1:950494/68‑1 (MQ=255)aTTAAATGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGc < 1:1700268/68‑1 (MQ=255) ttAAACGCCAGCCTAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGt < 1:2923529/69‑1 (MQ=255) ttAAACGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGt < 1:2410362/69‑1 (MQ=255) ttAAACGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGt < 1:349530/69‑1 (MQ=255) ttAAACGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGt < 1:3341368/69‑1 (MQ=255) ttAAACGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGt < 1:2283313/69‑1 (MQ=255) ttAAACGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGt < 1:1310079/69‑1 (MQ=255) ccAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGa < 1:2532850/52‑1 (MQ=255) ccAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGa < 1:2465828/52‑1 (MQ=255) cGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCATGCGGGTCAGGGCGTCACGGAAGGCCg < 1:150444/69‑1 (MQ=255) cGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCg < 1:1749296/69‑1 (MQ=255) cGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCg < 1:1088702/69‑1 (MQ=255) cGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCg < 1:1108767/69‑1 (MQ=255) cGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCg < 1:595157/69‑1 (MQ=255) cGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCg < 1:3000698/69‑1 (MQ=255) cGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCg < 1:873022/69‑1 (MQ=255) cGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCg < 1:2614864/69‑1 (MQ=255) cGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCg < 1:135447/69‑1 (MQ=255) cGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCg < 1:1983830/69‑1 (MQ=255) cGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCg < 1:2086397/69‑1 (MQ=255) gattGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCg < 1:3350097/65‑1 (MQ=255) gAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCg < 1:74170/68‑1 (MQ=255) gAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGc < 1:2693302/54‑1 (MQ=255) tGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAata > 1:1148921/1‑69 (MQ=255) cTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAg > 1:3137814/1‑67 (MQ=255) cATTTGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCa < 1:2258386/69‑1 (MQ=255) aTTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCa > 1:2652041/1‑68 (MQ=255) aTTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAg > 1:2310804/1‑69 (MQ=255) aTTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAg > 1:967366/1‑69 (MQ=255) aTTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAg > 1:1104507/1‑69 (MQ=255) aTTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAg > 1:1417095/1‑69 (MQ=255) aTTTGACCTTTGTTCAGGCGGGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAg > 1:902969/1‑69 (MQ=255) tGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAAg < 1:78144/36‑1 (MQ=255) tGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAAg < 1:2186769/36‑1 (MQ=255) tGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAAg < 1:1024608/36‑1 (MQ=255) ccTTTGTTCAGGCGAGTCATGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCgag < 1:3402908/68‑1 (MQ=255) ccTTTGTTCAGGCGAGTCAGTGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCgag < 1:947504/68‑1 (MQ=255) ccTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCgag < 1:981926/68‑1 (MQ=255) ccTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCgag < 1:3044227/68‑1 (MQ=255) ccTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCgag < 1:2772039/68‑1 (MQ=255) ccTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCgag < 1:2224945/68‑1 (MQ=255) ccTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCgag < 1:1213616/68‑1 (MQ=255) ttGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATcg > 1:852594/1‑69 (MQ=255) cAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCgccgcc > 1:3388272/1‑69 (MQ=255) cAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCgccgcc > 1:3238553/1‑69 (MQ=255) cAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCgccgcc > 1:653440/1‑69 (MQ=255) cAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCgccgcc > 1:3055937/1‑69 (MQ=255) cAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCgccgcc > 1:2874611/1‑69 (MQ=255) cAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCgccgcc > 1:2814099/1‑69 (MQ=255) cAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCgccgcc > 1:191954/1‑69 (MQ=255) | ATTAAATGCCAGCCGAATGAAGAGTGAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAAGGCCGGATCGAAAATATCTGGTGTAGCCCAGCCGAGATCGCCGCC > minE/51248‑51369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |