| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 435,666 | T→C | 55.0% | A146A (GCA→GCG) | ybfF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 435,666 | 0 | T | C | 55.0% | 10.9 / 46.1 | 60 | A146A (GCA→GCG) | ybfF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (22/5); new base C (23/10); total (45/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
AACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTC > minE/435603‑435728 | aacaaAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGGTGCTGGCGCATTATtgctgctgc > 1:1821053/1‑69 (MQ=255)aacaaAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATtgctgctgc > 1:263422/1‑69 (MQ=255)aacaaAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATtgctgctgc > 1:990399/1‑69 (MQ=255)aacaaAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATtgctgctgc > 1:411683/1‑69 (MQ=255)aacaaAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATtgctgctgc > 1:3352355/1‑69 (MQ=255)aacaaAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATtgctgctgc > 1:3198036/1‑69 (MQ=255)aacaaAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATtgctgctgc > 1:2290082/1‑69 (MQ=255)aacaaAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATtgctgc > 1:1410178/1‑66 (MQ=255) aaGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATtgctgct > 1:1831667/1‑62 (MQ=255) aaGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCtgcttgct > 1:2180823/1‑69 (MQ=255) aaGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCtgcttgct > 1:300334/1‑69 (MQ=255) aaGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCtgcttgct > 1:2781371/1‑69 (MQ=255) aaGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCtgcttgct > 1:637932/1‑69 (MQ=255) aaGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCtgcttgc > 1:2543251/1‑68 (MQ=255) aaGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCtgcttgc > 1:1835780/1‑68 (MQ=255) gcagAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGtt < 1:555535/68‑1 (MQ=255) aaCTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGttt > 1:2506266/1‑64 (MQ=255) aaCTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCg > 1:343916/1‑69 (MQ=255) aaCTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCg > 1:2697338/1‑69 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCtgcttgct > 1:2676515/1‑39 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGtt > 1:869965/1‑47 (MQ=39) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTctg > 1:1775197/1‑55 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGAtt > 1:814097/1‑60 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGAt > 1:1840360/1‑59 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:1029594/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:343495/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:283873/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:2744700/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:1347215/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:2226006/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:337376/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:539121/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:181143/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:687698/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:1671225/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:1497523/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:1487041/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGAct > 1:3321612/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGAct > 1:1490669/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGAct > 1:256404/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGAct > 1:1410193/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTAACTGAct > 1:1680903/1‑67 (MQ=255) tcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTG‑TTGTGCGTCTGATTCACTGACtgcg < 1:939566/68‑1 (MQ=255) cATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACcgcg > 1:806109/1‑68 (MQ=255) cATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACcgcg > 1:2858807/1‑68 (MQ=255) cATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACcgcg > 1:2681675/1‑68 (MQ=255) ttAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGtt < 1:1617657/68‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:1343416/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:653625/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:1716926/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:560281/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:466449/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:462420/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:1347190/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:2289266/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:2630284/66‑1 (MQ=255) aaGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGtt > 1:644266/1‑66 (MQ=255) gATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATa < 1:838916/68‑1 (MQ=255) gATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATa < 1:360311/68‑1 (MQ=255) gATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATa < 1:677383/68‑1 (MQ=255) ttattGATGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtct > 1:225877/1‑69 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:1193360/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:92441/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:16481/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:1821557/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:2001632/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:209260/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:220209/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:3200257/1‑68 (MQ=255) | AACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTC > minE/435603‑435728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |