| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 435,681 | A→T | 61.3% | T141T (ACT→ACA) | ybfF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 435,681 | 0 | A | T | 61.3% | 50.7 / 73.0 | 80 | T141T (ACT→ACA) | ybfF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (24/7); new base T (22/27); total (46/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.35e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01 | |||||||||||
GCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGACGGCGTACGTGA > minE/435617‑435747 | gcagAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGtt < 1:555535/68‑1 (MQ=255) aaCTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGttt > 1:2506266/1‑64 (MQ=255) aaCTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCg > 1:343916/1‑69 (MQ=255) aaCTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCg > 1:2697338/1‑69 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGtt > 1:869965/1‑47 (MQ=39) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTctg > 1:1775197/1‑55 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGAtt > 1:814097/1‑60 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGAt > 1:1840360/1‑59 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:1671225/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:1029594/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:181143/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:2226006/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:2744700/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:283873/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:337376/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:687698/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:343495/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:539121/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:1347215/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:1497523/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGActg > 1:1487041/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGAct > 1:3321612/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGAct > 1:1410193/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGAct > 1:256404/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGAct > 1:1490669/1‑67 (MQ=255) cttcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTAACTGAct > 1:1680903/1‑67 (MQ=255) tcATTAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTG‑TTGTGCGTCTGATTCACTGACtgcg < 1:939566/68‑1 (MQ=255) cATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACcgcg > 1:806109/1‑68 (MQ=255) cATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACcgcg > 1:2858807/1‑68 (MQ=255) cATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACcgcg > 1:2681675/1‑68 (MQ=255) ttAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGtt < 1:1617657/68‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:653625/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:560281/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:466449/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:2289266/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:462420/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:1716926/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:2630284/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:1343416/66‑1 (MQ=255) ttAAGATGCTGGCGCATTATTGCCGCTGCTTGCTGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:1347190/66‑1 (MQ=255) aaGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGtt > 1:644266/1‑66 (MQ=255) gATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATa < 1:838916/68‑1 (MQ=255) gATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATa < 1:677383/68‑1 (MQ=255) gATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATa < 1:360311/68‑1 (MQ=255) ttattGATGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtct > 1:225877/1‑69 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:1821557/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:220209/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:16481/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:209260/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:3200257/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:92441/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:2001632/1‑68 (MQ=255) attGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAAtctc > 1:1193360/1‑68 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:2155526/67‑1 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:1526313/67‑1 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:2174042/67‑1 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:2066711/67‑1 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:415967/67‑1 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:1187917/67‑1 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:1327194/67‑1 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:2616984/67‑1 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:1331095/67‑1 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:3327397/67‑1 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:1895712/67‑1 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:3097338/67‑1 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:3083196/67‑1 (MQ=255) cgctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:2974239/67‑1 (MQ=255) gctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:1584395/67‑1 (MQ=255) gctgctTGCTGGCGTGTTTGTGCGTCTGATTCACTGACTGCGTTGATGGCCGCGAAAATCTcatcat < 1:1778075/67‑1 (MQ=255) ttgctGGCGTGTTTGTGCGTCTGATTCACTGACtgcg < 1:3014410/37‑1 (MQ=25) tgctGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGACggat > 1:989951/1‑66 (MQ=255) tgctGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGACggat > 1:545270/1‑66 (MQ=255) tgctGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGACggat > 1:3386499/1‑66 (MQ=255) tgctGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGACggat > 1:3248816/1‑66 (MQ=255) tgctGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGACggat > 1:3172434/1‑66 (MQ=255) tgctGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGACggat > 1:207521/1‑66 (MQ=255) tgctGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGACggat > 1:1107987/1‑66 (MQ=255) tgctGACGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGACggat > 1:1084782/1‑66 (MQ=255) gAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGACGGCGTACGTGa < 1:3412107/68‑1 (MQ=255) gAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGACGGCGTACGTGa < 1:1432032/68‑1 (MQ=255) | GCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGACGGCGTACGTGA > minE/435617‑435747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |