| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 435,813 | T→A | 34.6% | A97A (GCA→GCT) | ybfF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 435,813 | 0 | T | A | 34.6% | 70.3 / 64.7 | 84 | A97A (GCA→GCT) | ybfF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (21/33); new base A (9/20); total (30/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
ATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAG > minE/435747‑435881 | aTAGTCGACCGGCGCGATATCGATCGCCACTAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGc < 1:363184/69‑1 (MQ=255)aTAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGc < 1:1800670/69‑1 (MQ=255)aTAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGc < 1:1798406/69‑1 (MQ=255)aTAGTCGACCGGCGCGATATAGATCGCCACCAGTTTATCGATGCGATCGGAGGATAGTGCAGTAAGTGc < 1:995257/69‑1 (MQ=255) gTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCaa > 1:1872537/1‑68 (MQ=255) gTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCAt > 1:1086598/1‑69 (MQ=255) gTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCAt > 1:2512218/1‑69 (MQ=255) gTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCAt > 1:1996639/1‑69 (MQ=255) gTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCAt > 1:1856927/1‑69 (MQ=255) gTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCAt > 1:1725172/1‑69 (MQ=255) gTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCAt > 1:586351/1‑69 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGTAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:3047010/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:145890/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:3179921/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:3170360/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:230274/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:1229678/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:1290876/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:3059844/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:518403/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:993637/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:974479/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:1464574/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:1657597/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:636981/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:2650061/62‑1 (MQ=255) atcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCtt < 1:2646078/62‑1 (MQ=255) tcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATg < 1:2492995/68‑1 (MQ=255) tcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATg < 1:176115/68‑1 (MQ=255) tcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATg < 1:2546086/68‑1 (MQ=255) tcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATg < 1:1533256/68‑1 (MQ=255) cgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGa > 1:1527876/1‑69 (MQ=255) cgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGa > 1:3082243/1‑69 (MQ=255) cgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGa > 1:1291169/1‑69 (MQ=255) cgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGa > 1:569878/1‑69 (MQ=255) caccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGt < 1:1129022/68‑1 (MQ=255) caccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGt < 1:3192979/68‑1 (MQ=255) caccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGt < 1:2415669/68‑1 (MQ=255) caccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGt < 1:2478513/68‑1 (MQ=255) caccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGt < 1:764051/68‑1 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:337607/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:3397462/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:554452/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:3035435/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:1095962/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:2839000/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:84979/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:2490602/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:1701250/1‑69 (MQ=255) ggCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGAt > 1:3285094/1‑69 (MQ=255) tAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctg > 1:2576846/1‑69 (MQ=255) gCAGTAAGCGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:3107478/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1615621/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1650056/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1504144/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1778244/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:401358/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1818648/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1856706/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1954641/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:929972/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1458924/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:3211878/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2064077/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2628789/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:227337/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2574658/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2365041/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2494385/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2571506/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2681687/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:278070/67‑1 (MQ=255) gTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa < 1:129370/69‑1 (MQ=255) gTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa < 1:216644/69‑1 (MQ=255) gTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa < 1:1813870/69‑1 (MQ=255) gTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa < 1:3014084/69‑1 (MQ=255) tAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa > 1:2541980/1‑68 (MQ=255) tAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa > 1:2827800/1‑68 (MQ=255) tAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa > 1:3069620/1‑68 (MQ=255) tAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa > 1:136106/1‑68 (MQ=255) aGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATa > 1:2802751/1‑39 (MQ=255) aGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATa > 1:930986/1‑39 (MQ=255) aGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa > 1:290931/1‑66 (MQ=255) aGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa > 1:295301/1‑66 (MQ=255) tGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCag < 1:1691128/69‑1 (MQ=255) tGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCag < 1:1972089/69‑1 (MQ=255) | ATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAG > minE/435747‑435881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |