| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 435,831 | G→A | 38.3% | G91G (GGC→GGT) | ybfF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 435,831 | 0 | G | A | 38.3% | 42.6 / 49.5 | 79 | G91G (GGC→GGT) | ybfF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (23/25); new base A (9/21); total (32/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
TCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATC > minE/435770‑435887 | tcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATg < 1:176115/68‑1 (MQ=255)tcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATg < 1:1533256/68‑1 (MQ=255)tcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATg < 1:2546086/68‑1 (MQ=255)tcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATg < 1:2492995/68‑1 (MQ=255) cgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGa > 1:3082243/1‑69 (MQ=255) cgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGa > 1:569878/1‑69 (MQ=255) cgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGa > 1:1527876/1‑69 (MQ=255) cgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGa > 1:1291169/1‑69 (MQ=255) caccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGt < 1:764051/68‑1 (MQ=255) caccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGt < 1:2478513/68‑1 (MQ=255) caccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGt < 1:2415669/68‑1 (MQ=255) caccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGt < 1:3192979/68‑1 (MQ=255) caccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGt < 1:1129022/68‑1 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:2490602/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:1095962/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:3035435/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:337607/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:3397462/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:2839000/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:554452/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:84979/1‑69 (MQ=255) gATCGGGGGCTAGTGCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCtt > 1:1701250/1‑69 (MQ=255) ggCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGAt > 1:3285094/1‑69 (MQ=255) tAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctg > 1:2576846/1‑69 (MQ=255) gCAGTAAGCGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:3107478/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2365041/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2628789/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2574658/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2571506/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:929972/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2494385/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2681687/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:278070/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1458924/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1504144/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1615621/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:227337/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1650056/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:3211878/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:2064077/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1954641/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1856706/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:401358/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1818648/67‑1 (MQ=255) gCAGTAAGAGCCATTACCGCTTTACCACCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgct < 1:1778244/67‑1 (MQ=255) gTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa < 1:1813870/69‑1 (MQ=255) gTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa < 1:3014084/69‑1 (MQ=255) gTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa < 1:129370/69‑1 (MQ=255) gTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa < 1:216644/69‑1 (MQ=255) tAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa > 1:3069620/1‑68 (MQ=255) tAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa > 1:2541980/1‑68 (MQ=255) tAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa > 1:136106/1‑68 (MQ=255) tAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa > 1:2827800/1‑68 (MQ=255) aGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATa > 1:930986/1‑39 (MQ=255) aGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATa > 1:2802751/1‑39 (MQ=255) aGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa > 1:295301/1‑66 (MQ=255) aGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCa > 1:290931/1‑66 (MQ=255) tGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCag < 1:1691128/69‑1 (MQ=255) tGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCag < 1:1972089/69‑1 (MQ=255) aCCGCTTTACCTCCCATGGAGTGACCGATAAATGTTGCTTTGTCGa < 1:604283/46‑1 (MQ=255) aCCGCTTTACCGCCCTTGGAGTGACCGATAAATGTTGCTTTGTCg < 1:2481534/45‑1 (MQ=255) aCCGCTTTACCGCCCATGGAGTTACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATc > 1:1380991/1‑68 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCg < 1:2186478/45‑1 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGa < 1:1121199/46‑1 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGa < 1:1224528/46‑1 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGa < 1:577962/46‑1 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGa < 1:521211/46‑1 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGa < 1:3258184/46‑1 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGa < 1:2137730/46‑1 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGa < 1:2446727/46‑1 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGa < 1:3057373/46‑1 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATc > 1:2112813/1‑68 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATc > 1:1889725/1‑68 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATc > 1:1849037/1‑68 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATc > 1:3195707/1‑68 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATc > 1:2619366/1‑68 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATc > 1:1241277/1‑68 (MQ=255) aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATc > 1:2645481/1‑68 (MQ=255) aCCGCTTAACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATc > 1:1732792/1‑68 (MQ=255) | TCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATC > minE/435770‑435887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |