| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 441,078 | A→G | 62.6% | G163G (GGT→GGC) | potE ← | putrescine/proton symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 441,078 | 0 | A | G | 62.6% | 33.8 / 31.5 | 56 | G163G (GGT→GGC) | potE | putrescine/proton symporter |
| Reads supporting (aligned to +/- strand): ref base A (14/7); new base G (27/8); total (41/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATGCG > minE/441015‑441137 | atgCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACcgac > 1:7307/1‑69 (MQ=255) aTTCCAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGcct > 1:2498096/1‑60 (MQ=255) aTTCCAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGcct > 1:2478896/1‑60 (MQ=255) aTTCCAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGcct > 1:2754060/1‑60 (MQ=255) aTTCCAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGcct > 1:1123511/1‑60 (MQ=255) aTTCCAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGcct > 1:1192740/1‑60 (MQ=255) aTTCCAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGcct > 1:535071/1‑60 (MQ=255) aTTCCAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGcct > 1:3166485/1‑60 (MQ=255) aTTCCAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGcct > 1:1252801/1‑60 (MQ=255) aTTCCAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGcct > 1:3393584/1‑60 (MQ=255) aTTCCAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGcct > 1:3237410/1‑60 (MQ=255) aTTCCAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGcct > 1:1846200/1‑60 (MQ=255) aTTCCAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGcct > 1:3340269/1‑60 (MQ=255) aTTCCAGGAATCAACGTAAAGTGTAGGGCTAAACCAGAACCAGCCGATAATGCACAGGcct > 1:2800480/1‑60 (MQ=255) ccAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGaat > 1:3101177/1‑69 (MQ=255) ccAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGaat < 1:1969284/69‑1 (MQ=255) ccAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGaat < 1:1453806/69‑1 (MQ=255) ccAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGaat < 1:1237639/69‑1 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:1499974/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:1454413/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:2779645/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:1940286/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:196640/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:1380532/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:3209070/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:2264645/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:1097818/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:1069910/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:3001096/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaat > 1:2189682/1‑68 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaat > 1:942764/1‑68 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaa > 1:574001/1‑67 (MQ=255) aaCGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGAccc > 1:3325297/1‑68 (MQ=255) cGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGATCGACGACCGGAATAATGAccccc < 1:1342493/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:3157311/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:2222550/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:2626151/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:2153518/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:2042939/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:1878285/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:1771044/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:1044461/68‑1 (MQ=255) aaaCCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACt < 1:2475284/69‑1 (MQ=255) aaaCCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACt < 1:1563941/69‑1 (MQ=255) aaaCCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACt < 1:1295316/69‑1 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGc > 1:289816/1‑57 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:867342/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:992940/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:792781/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:959992/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:3331839/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:3293069/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:2973051/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:2623765/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:123235/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:1057348/1‑68 (MQ=255) | ATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATGCG > minE/441015‑441137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |