| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 441,081 | G→T | 52.3% | V162V (GTC→GTA) | potE ← | putrescine/proton symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 441,081 | 0 | G | T | 52.3% | ‑4.9 / 49.3 | 42 | V162V (GTC→GTA) | potE | putrescine/proton symporter |
| Reads supporting (aligned to +/- strand): ref base G (13/7); new base T (14/8); total (27/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
ATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATGCG > minE/441015‑441137 | atgCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACcgac > 1:7307/1‑69 (MQ=255) ccAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGaat < 1:1453806/69‑1 (MQ=255) ccAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGaat < 1:1969284/69‑1 (MQ=255) ccAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGaat > 1:3101177/1‑69 (MQ=255) ccAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGaat < 1:1237639/69‑1 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:1097818/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:2779645/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:1380532/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:1454413/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:1499974/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:3209070/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:3001096/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:1069910/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:1940286/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:196640/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaata > 1:2264645/1‑69 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaat > 1:2189682/1‑68 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaat > 1:942764/1‑68 (MQ=255) cAGGAATCAACGTAAAGTGTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGaa > 1:574001/1‑67 (MQ=255) aaCGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGAccc > 1:3325297/1‑68 (MQ=255) cGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGATCGACGACCGGAATAATGAccccc < 1:1342493/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:3157311/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:2626151/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:2042939/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:2222550/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:2153518/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:1878285/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:1771044/68‑1 (MQ=255) tgTCGGGCTAAACCAGAACCAGCCGATAATGCACAGGCCTACCACCGGAATAATGACCCCCCACACGGt < 1:1044461/68‑1 (MQ=255) aaaCCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACt < 1:2475284/69‑1 (MQ=255) aaaCCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACt < 1:1563941/69‑1 (MQ=255) aaaCCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACt < 1:1295316/69‑1 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGc > 1:289816/1‑57 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:867342/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:992940/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:792781/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:959992/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:3331839/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:3293069/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:2973051/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:2623765/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:123235/1‑68 (MQ=255) atGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATgcg > 1:1057348/1‑68 (MQ=255) | ATGCGGATTCCAGGAATCAACGTACAGCGTCGGGCTAAACCAGAACCAGCCAATAATGCACAGACCGACGACCGGAATAATGACCCCCCACACGGTAATGCTACTGATTTGCCCGGTAATGCG > minE/441015‑441137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |