| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 443,210 | T→A | 19.6% | A184A (GCA→GCT) | speF ← | ornithine decarboxylase isozyme, inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 443,210 | 0 | T | A | 19.6% | 142.3 / 26.1 | 76 | A184A (GCA→GCT) | speF | ornithine decarboxylase isozyme, inducible |
| Reads supporting (aligned to +/- strand): ref base T (23/38); new base A (7/8); total (30/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01 | |||||||||||
AAACCACTTTGTTAGAAGATGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGT > minE/443143‑443274 | aaaCCACTTTGTTAGAAGATGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACtttt < 1:148212/68‑1 (MQ=255)aaaCCACTTTGTTAGAAGATGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACtttt < 1:2205925/68‑1 (MQ=255)aaaCCACTTTGTTAGAAGATGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACtttt < 1:36240/68‑1 (MQ=255)aaaCCACTTTGTTAGAAGATGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACtttt < 1:2592721/68‑1 (MQ=255) aCTTTGTTAGAAGATGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCa > 1:1156555/1‑69 (MQ=255) aCTTTGTTAGAAGATGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCa > 1:883306/1‑69 (MQ=255) aCTTTGTTAGAAGATGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCa > 1:829464/1‑69 (MQ=255) aCTTTGTTAGAAGATGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCa < 1:2495518/69‑1 (MQ=255) aCTTTGTTAGAAGATGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCa > 1:39510/1‑69 (MQ=255) aCTTTGTTAGAAGATGAAGTCCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCa < 1:3397075/69‑1 (MQ=255) ttAGAAGATGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGtt < 1:3225010/67‑1 (MQ=255) agaTGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGc < 1:138042/69‑1 (MQ=255) agaTGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGc < 1:528290/69‑1 (MQ=255) agaTGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGc < 1:3314136/69‑1 (MQ=255) agaTGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGc < 1:295749/69‑1 (MQ=255) aTGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCaa < 1:874399/69‑1 (MQ=255) aTGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCaa < 1:2278566/69‑1 (MQ=255) aTGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCaa < 1:3188385/69‑1 (MQ=255) gAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATg < 1:2103727/69‑1 (MQ=255) aTTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGgcgc > 1:2411924/1‑69 (MQ=255) aTTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGgcgc > 1:2013328/1‑69 (MQ=255) aTTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGgcgc > 1:1490070/1‑69 (MQ=255) aTTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGgcgc > 1:1741496/1‑69 (MQ=255) aTTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGgcgc > 1:1617589/1‑69 (MQ=255) aTTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGgcgc > 1:3256584/1‑69 (MQ=255) aTTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGgcgc > 1:139451/1‑69 (MQ=255) aTTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGgcgc > 1:1109897/1‑69 (MQ=255) ttAAAACGAAGTAGGTTTTATCGGCATTAAACACtttt < 1:3162613/38‑1 (MQ=255) aaCGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCtt < 1:3200825/68‑1 (MQ=255) aaCGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCtt < 1:2019666/68‑1 (MQ=255) aaCGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCtt < 1:2252819/68‑1 (MQ=255) aaCGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCtt < 1:937563/68‑1 (MQ=255) aCGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCtt < 1:2870704/67‑1 (MQ=255) ggtttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCaa < 1:664347/41‑1 (MQ=255) ttttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagca < 1:993111/69‑1 (MQ=255) ttttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagca < 1:3270332/69‑1 (MQ=255) ttttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagca < 1:2003341/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGTATcagcag < 1:2117492/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:1027132/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:3093638/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:1373015/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:629468/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:2895602/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:2691573/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:112846/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagca > 1:221263/1‑68 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATc > 1:2345477/1‑64 (MQ=255) ttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATc > 1:2794263/1‑63 (MQ=255) ttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATc > 1:1429592/1‑63 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:732949/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:2589691/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:1188877/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:3036427/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:951395/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:3334457/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:1468049/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGAAGATCGcc < 1:1335858/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTAGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:844562/69‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:1765438/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:1458228/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:1461687/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:1841596/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:2229102/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:2981123/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:2089219/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:2517627/68‑1 (MQ=255) aaaCACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGc > 1:1457489/1‑69 (MQ=255) aaaCACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGc > 1:360799/1‑69 (MQ=255) aaaCACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGc > 1:2932650/1‑69 (MQ=255) aaaCACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGc > 1:787286/1‑69 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATcagaa > 1:1982993/1‑53 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:3301183/1‑68 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:1189236/1‑68 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:2524431/1‑68 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:2602378/1‑68 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:1318859/1‑68 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:352689/1‑68 (MQ=255) cacTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTAc < 1:2519555/69‑1 (MQ=255) cTTTTGCCGCATGTTGCTGTGCAATGCATGTCGCGCCTTCGTGAATc > 1:1257114/1‑47 (MQ=255) cTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGt > 1:2940839/1‑69 (MQ=255) cTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGt > 1:2286845/1‑69 (MQ=255) | AAACCACTTTGTTAGAAGATGAAGTGCCATTTAAAACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGT > minE/443143‑443274 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |