| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 443,240 | G→A | 26.1% | G174G (GGC→GGT) | speF ← | ornithine decarboxylase isozyme, inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 443,240 | 0 | G | A | 26.1% | 66.2 / 18.3 | 57 | G174G (GGC→GGT) | speF | ornithine decarboxylase isozyme, inducible |
| Reads supporting (aligned to +/- strand): ref base G (16/26); new base A (7/8); total (23/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCC > minE/443177‑443306 | aaCGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCtt < 1:2252819/68‑1 (MQ=255)aaCGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCtt < 1:2019666/68‑1 (MQ=255)aaCGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCtt < 1:937563/68‑1 (MQ=255)aaCGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCtt < 1:3200825/68‑1 (MQ=255) aCGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCtt < 1:2870704/67‑1 (MQ=255) ttttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagca < 1:993111/69‑1 (MQ=255) ttttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagca < 1:2003341/69‑1 (MQ=255) ttttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagca < 1:3270332/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGTATcagcag < 1:2117492/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:1373015/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:629468/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:3093638/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:2895602/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:112846/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:2691573/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagcag < 1:1027132/69‑1 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATcagca > 1:221263/1‑68 (MQ=255) tttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATc > 1:2345477/1‑64 (MQ=255) ttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATc > 1:1429592/1‑63 (MQ=255) ttATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATc > 1:2794263/1‑63 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:951395/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:732949/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:3036427/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:3334457/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:2589691/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:1468049/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:1188877/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGAAGATCGcc < 1:1335858/69‑1 (MQ=255) ggCATTAAACACTTTTGCCGCATGTAGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGcc < 1:844562/69‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:1458228/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:1461687/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:2981123/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:1765438/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:1841596/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:2229102/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:2517627/68‑1 (MQ=255) cATTAAACACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCAccc < 1:2089219/68‑1 (MQ=255) aaaCACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGc > 1:2932650/1‑69 (MQ=255) aaaCACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGc > 1:1457489/1‑69 (MQ=255) aaaCACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGc > 1:360799/1‑69 (MQ=255) aaaCACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGc > 1:787286/1‑69 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATcagaa > 1:1982993/1‑53 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:1318859/1‑68 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:3301183/1‑68 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:352689/1‑68 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:1189236/1‑68 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:2602378/1‑68 (MQ=255) aaCACTTTAGCCGCATGTTGCTGTGCAATGCATGGCGCACCTTCATGAATCAGAAGATCACCCATTGca > 1:2524431/1‑68 (MQ=255) cacTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTAc < 1:2519555/69‑1 (MQ=255) cTTTTGCCGCATGTTGCTGTGCAATGCATGTCGCGCCTTCGTGAATc > 1:1257114/1‑47 (MQ=255) cTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGt > 1:2940839/1‑69 (MQ=255) cTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGt > 1:2286845/1‑69 (MQ=255) ggCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAgc > 1:1107246/1‑69 (MQ=255) ggCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAgc > 1:3417022/1‑69 (MQ=255) ggCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAgc > 1:317494/1‑69 (MQ=255) ggCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAgc > 1:3161415/1‑69 (MQ=255) ggCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAgc > 1:2963793/1‑69 (MQ=255) gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGcc < 1:361372/69‑1 (MQ=255) gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGcc < 1:581962/69‑1 (MQ=255) gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGcc < 1:3301474/69‑1 (MQ=255) gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGcc < 1:916189/69‑1 (MQ=255) gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGcc < 1:1830118/69‑1 (MQ=255) gcgcCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGcc < 1:1852498/69‑1 (MQ=255) | AACGAAGTAGGTTTTATCGGCATTAAACACTTTTGCCGCATGTTGCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCC > minE/443177‑443306 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |