| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 445,418 | T→C | 100% | E766E (GAA→GAG) | kdpD ← | fused sensory histidine kinase in two‑component regulatory system with KdpE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 445,418 | 0 | T | C | 92.4% | 22.0 / ‑4.3 | 13 | E766E (GAA→GAG) | kdpD | fused sensory histidine kinase in two‑component regulatory system with KdpE |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (0/12); total (1/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCC > minE/445350‑445480 | gcgTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGt < 1:3074079/69‑1 (MQ=255) ggttAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGCTCTGGCAGAGaaa < 1:1710525/67‑1 (MQ=255) ggttAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGCTCTGGCAGAGaaa < 1:1946028/67‑1 (MQ=255) ggttAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGCTCTGGCAGAGaaa < 1:2110061/67‑1 (MQ=255) ggttAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGCTCTGGCAGAGaaa < 1:2180661/67‑1 (MQ=255) ggttAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGCTCTGGCAGAGaaa < 1:2560114/67‑1 (MQ=255) ggttAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGCTCTGGCAGAGaaa < 1:2684012/67‑1 (MQ=255) ggttAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGCTCTGGCAGAGaaa < 1:3155955/67‑1 (MQ=255) ggttAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGCTCTGGCAGAGaaa < 1:792514/67‑1 (MQ=255) ggttAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGCTCTGGCAGAGaaa < 1:930831/67‑1 (MQ=255) ggttAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGGCAGCGGCTCTGGCAGAGaaa < 1:2543767/67‑1 (MQ=255) gTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGCTCTGGCAGAGaaa < 1:2706540/54‑1 (MQ=255) aaCGTGGATTAAGGTCAGCGGCTCTGGCAGAGaaa < 1:397271/35‑1 (MQ=255) aGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCTGTTCCAGCATTTGCAGCGCGCTGcc > 1:1057419/1‑68 (MQ=255) | GCGTTCTCCAGCAGATTAATCAGCACCCGTTCAAAGAGTGGCCCGTCAACGTGGATTAAGGTCAGCGGTTCTGGCAGAGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCC > minE/445350‑445480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |