| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 445,484 | T→C | 56.1% | V744V (GTA→GTG) | kdpD ← | fused sensory histidine kinase in two‑component regulatory system with KdpE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 445,484 | 0 | T | C | 56.1% | 10.9 / 35.8 | 50 | V744V (GTA→GTG) | kdpD | fused sensory histidine kinase in two‑component regulatory system with KdpE |
| Reads supporting (aligned to +/- strand): ref base T (10/12); new base C (4/24); total (14/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.53e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATA > minE/445427‑445547 | agaAAGATTGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACCACTTCTTCCa > 1:2827993/1‑68 (MQ=255)agaAAGATTGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACCACTTCTTCCAg > 1:940090/1‑69 (MQ=255)agaAAGATTGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACCACTTCTTCCAg > 1:2454250/1‑69 (MQ=255)agaAAGATTGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACCACTTCTTCCAg > 1:3415099/1‑69 (MQ=255) ttGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTTCCGACTACTTCTTCCAGCGTTAAc < 1:2396229/69‑1 (MQ=255) ttGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAAc < 1:2328282/69‑1 (MQ=255) ttGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAAc < 1:1417814/69‑1 (MQ=255) ttGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAAc < 1:817775/69‑1 (MQ=255) ttGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAAc < 1:1246334/69‑1 (MQ=255) ttGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAAc < 1:1640513/69‑1 (MQ=255) aTGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCAc > 1:1325851/1‑69 (MQ=255) aTGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCAc > 1:2515655/1‑69 (MQ=255) aTGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCAc > 1:707689/1‑69 (MQ=255) cgacgaTAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACtcttt < 1:2257118/69‑1 (MQ=255) aaaCCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCaa > 1:2272014/1‑68 (MQ=255) gTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAgc > 1:2960362/1‑69 (MQ=255) tGCAGCGCGCTGCCGACTACTTCTTCCATCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGa > 1:2092128/1‑69 (MQ=255) tGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACtctttc > 1:2920677/1‑45 (MQ=255) tGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGa > 1:561032/1‑69 (MQ=255) tGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGa > 1:1533727/1‑69 (MQ=255) tGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGa > 1:2435880/1‑69 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:2828260/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:799801/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:1170635/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:3082984/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:3379840/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:3394483/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:580201/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:658497/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:704801/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:741082/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:275532/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:1251760/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:1315787/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:1412013/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:1889575/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:1954883/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:2104334/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:2114041/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:2176962/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:2209128/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:2284351/42‑1 (MQ=255) tGCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:2576844/42‑1 (MQ=255) gCAGCGCGCTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:302227/41‑1 (MQ=255) cgcgcTGCCGACCACTTCTTCCAGCGTTAACCActct < 1:2814172/37‑1 (MQ=255) gACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCata < 1:18318/67‑1 (MQ=255) gACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCata < 1:1759716/67‑1 (MQ=255) gACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCata < 1:1625432/67‑1 (MQ=255) gACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCata < 1:243477/67‑1 (MQ=255) gACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCata < 1:116830/67‑1 (MQ=255) | AGAAAGATTGATGGGCGACGATAAACCCGGTTCCAGCATTTGCAGCGCGCTGCCGACTACTTCTTCCAGCGTTAACCACTCTTTCTTCAAATTAAAGCCGCCGGACTGAATTCGCGCCATA > minE/445427‑445547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |