| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 447,521 | T→C | 54.8% | R65R (CGA→CGG) | kdpD ← | fused sensory histidine kinase in two‑component regulatory system with KdpE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 447,521 | 0 | T | C | 54.8% | 5.0 / 30.9 | 42 | R65R (CGA→CGG) | kdpD | fused sensory histidine kinase in two‑component regulatory system with KdpE |
| Reads supporting (aligned to +/- strand): ref base T (6/13); new base C (16/7); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGCCGCCCACGGTACGCCTGGCGTTTTAACGGCAGAACAGCCAGCCCTTCCAGCATGGCGGCGGTATCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCGCTGGGCTTC > minE/447449‑447586 | atGCCGCCCACGGTACGCCTGGCGTTTTAACGGCAGAA‑‑‑‑CAGCCCTTCCAGCATGGCGGCGGTATCtttt > 1:2066667/1‑69 (MQ=255)atGCCGCCCACGGTACGCCTGGCGTTTTAACGGCAGAA‑‑‑‑CAGCCCTTCCAGCATGGCGGCGGTATCtttt > 1:270207/1‑69 (MQ=255)atGCCGCCCACGGTACGCCTGGCGTTTTAACGGCAGAA‑‑‑‑CAGCCCTTCCAGCATGGCGGCGGTATCtttt > 1:1987021/1‑69 (MQ=255)atGCCGCCCACGGTACGCCTGGCGTTTTAACGGCAGAA‑‑‑‑CAGCCCTTCCAGCATGGCGGCGGTATCtttt > 1:3106340/1‑69 (MQ=255)atGCCGCCCACGGTACGCCTGGCGTTTTAACGGCAGAA‑‑‑‑CAGCCCTTCCAGCATGGCGGCGGTATCtttt > 1:524938/1‑69 (MQ=255) cGCCTGGCGTTTTAACGGCAGAACAGCCAGCCCTTCCAGCATGGCGGCGGTATCTTTTCGCCCGTGGGt > 1:1361620/1‑69 (MQ=255) ttAACGGCAGAACAGCCAGCCCTTCCAGCATGGCGGCGGTATCTTTTCGCCCGTGGGTTTCTACCACg > 1:1474324/1‑68 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:3065060/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:2049308/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:3008477/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:2959331/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:2402362/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:3170829/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:2244194/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:2934089/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:2105531/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:2881784/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:2063331/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:1931892/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:414163/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:613646/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:1097519/1‑57 (MQ=255) tAACGGCAGAACAGCCAGACCTTTCAGCATGGCGGCGGTATCTTTCCGACCGTGGGt > 1:1056969/1‑57 (MQ=255) aCAGCCAGCCCTTCCAGCATGGCGGCGGTATCTTTTCGCCCGTGGGTTTCt > 1:2343130/1‑51 (MQ=255) aCAGCCAGCCCTTCCAGCATGGCGGCGGTATCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAata > 1:1926024/1‑69 (MQ=255) aCAGCCAGCCCTTCCAGCATGGCGGCGGTATCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAata > 1:937411/1‑69 (MQ=255) ccAGCATGGCGGCGGTATCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTgcg > 1:755811/1‑69 (MQ=255) ggcggTATCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTc < 1:2045275/68‑1 (MQ=255) ggcggTATCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTc < 1:2229375/68‑1 (MQ=255) ggcggTATCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTc < 1:470540/68‑1 (MQ=255) ggcggTATCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTc < 1:593848/68‑1 (MQ=255) ggcggTATCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTc < 1:2599199/68‑1 (MQ=255) ggcggTATCTTTCCTACCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCg < 1:1011434/69‑1 (MQ=255) ggcggTATCTTTCCGACCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCg < 1:2683148/69‑1 (MQ=255) ggcggTATCTTTCCGACCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCg < 1:105167/69‑1 (MQ=255) ggcggTATCTTTCCGACCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCg < 1:3044911/69‑1 (MQ=255) ggcggTATCTTTCCGACCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCg < 1:2413642/69‑1 (MQ=255) ggcggTATCTTTCCGACCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCg < 1:2263285/69‑1 (MQ=255) ggcggTATCTTTCCGACCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCg < 1:433436/69‑1 (MQ=255) gcggTATCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTc < 1:2707101/67‑1 (MQ=255) tCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCGCTGGGCt < 1:2703020/69‑1 (MQ=255) tCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCGCTGGGCt < 1:1127855/69‑1 (MQ=255) tCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCGCTGGGCt < 1:1667805/69‑1 (MQ=255) tCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCGCTGGGCt < 1:1705432/69‑1 (MQ=255) tCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCGCTGGGCt < 1:2417711/69‑1 (MQ=255) tCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCGCTGGGCt < 1:246374/69‑1 (MQ=255) tCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCGCTGGGCt < 1:2615796/69‑1 (MQ=255) ttttCTCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCGCTGGGCtt > 1:932343/1‑68 (MQ=255) ttttCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCGCTGGGCtt > 1:1010416/1‑68 (MQ=255) ttttCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCGCTGGGCTTc > 1:154776/1‑69 (MQ=255) ttttCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCGCTGGGCTTc > 1:1333548/1‑69 (MQ=255) | ATGCCGCCCACGGTACGCCTGGCGTTTTAACGGCAGAACAGCCAGCCCTTCCAGCATGGCGGCGGTATCTTTTCGCCCGTGGGTTTCTACCACGCCAACCACAATATCCAGCCCTTGCGCCCGCAGTCGCTGGGCTTC > minE/447449‑447586 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |