| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 449,111 | A→G | 100% | G409G (GGT→GGC) | kdpB ← | potassium translocating ATPase, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 449,111 | 0 | A | G | 83.5% | 56.5 / 3.1 | 36 | G409G (GGT→GGC) | kdpB | potassium translocating ATPase, subunit B |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base G (9/21); total (15/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.57e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCGGCGTGGCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTC > minE/449046‑449173 | aGCGGCGTGGCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGaccacc > 1:2248874/1‑68 (MQ=255)aGCGGCGTGGCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGaccacc > 1:915084/1‑68 (MQ=255)aGCGGCGTGGCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGaccacc > 1:2631354/1‑68 (MQ=255) gCTGCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTc > 1:1646830/1‑68 (MQ=255) gCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTATGGAAGTGACCACCGTTAGCCTc > 1:182133/1‑68 (MQ=255) gCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGtt > 1:2476725/1‑62 (MQ=255) gCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTc > 1:2760592/1‑68 (MQ=255) gCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTc > 1:2196470/1‑68 (MQ=255) gCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTc > 1:1849517/1‑68 (MQ=255) ggcaGGGAAGTGCCCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCAt > 1:443781/4‑69 (MQ=255) ggcaGGGAAGTGACCGCCGTTAGCCTCATCATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCAt > 1:2225445/4‑69 (MQ=255) ggcaGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGCCAGAACCTTTACg > 1:637880/4‑63 (MQ=255) ggcaGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCa > 1:1623003/4‑68 (MQ=255) ggcaGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCAt > 1:1754553/4‑69 (MQ=255) ggcaGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCAt > 1:2540300/4‑69 (MQ=255) ggcaGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCAt > 1:1759392/4‑69 (MQ=255) ggcaGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCAt > 1:1281331/4‑69 (MQ=255) ggcaGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCAt > 1:3212948/4‑69 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCATCATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:1050649/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:977065/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:3147274/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:3268597/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:330032/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:3341385/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:3376470/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:409155/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:435481/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:908938/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:2832415/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:1059267/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:1317115/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:1416924/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:1547845/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:1751327/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:1922011/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:2354441/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:2761724/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:3025353/67‑1 (MQ=255) caGGGAAGTGACCGCCGTTAGCCTCAACATGGCGCCGAATGGCATCGACAGAACCTTTACGGATCATg < 1:1529007/67‑1 (MQ=255) accaccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTc > 1:1807764/1‑66 (MQ=255) accaccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTc > 1:162942/1‑66 (MQ=255) accaccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTc > 1:3258227/1‑66 (MQ=255) accaccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTc > 1:78362/1‑66 (MQ=255) accaccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTc > 1:882696/1‑66 (MQ=255) accaccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTc > 1:112406/1‑66 (MQ=255) | AGCGGCGTGGCTCCCTGACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTC > minE/449046‑449173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |