| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 53,750 | C→T | 35.9% | K346K (AAG→AAA) | imp ← | exported protein required for envelope biosynthesis and integrity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 53,750 | 0 | C | T | 35.9% | 106.4 / 90.1 | 150 | K346K (AAG→AAA) | imp | exported protein required for envelope biosynthesis and integrity |
| Reads supporting (aligned to +/- strand): ref base C (42/54); new base T (22/32); total (64/86) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
Only 100 of 157 total aligned reads displayed.
GAAGTTTTGCACCGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACAC > minE/53684‑53818 | gAAGTTTTGCACCGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACtt > 1:1694744/1‑69 (MQ=255)gAAGTTTTGCACCGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACtt > 1:2381701/1‑69 (MQ=255)gAAGTTTTGCACCGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACtt > 1:2149549/1‑69 (MQ=255) gTTTTGCACCGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGt < 1:855655/68‑1 (MQ=255) ttGCACCGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTAt > 1:2746527/1‑68 (MQ=255) ccGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAAt < 1:2467716/69‑1 (MQ=255) ccGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAAt < 1:3337994/69‑1 (MQ=255) ccGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAAt < 1:3054471/69‑1 (MQ=255) ccGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAAt < 1:353282/69‑1 (MQ=255) ccGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAAt < 1:2183345/69‑1 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa > 1:2567812/1‑68 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa > 1:2631282/1‑68 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa < 1:986253/68‑1 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa < 1:215365/68‑1 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa < 1:2912273/68‑1 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa < 1:2313863/68‑1 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa > 1:2288229/1‑68 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa > 1:15113/1‑68 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa > 1:591515/1‑68 (MQ=255) gCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGa < 1:754405/68‑1 (MQ=255) cAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGt > 1:1312218/1‑69 (MQ=255) cAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGt > 1:2499399/1‑69 (MQ=255) cAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGt > 1:2819090/1‑69 (MQ=255) cAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGt > 1:3125072/1‑69 (MQ=255) cAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGt > 1:1772788/1‑69 (MQ=255) cAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGt > 1:960673/1‑69 (MQ=255) cAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGt > 1:1496126/1‑69 (MQ=255) cAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGt > 1:1672675/1‑69 (MQ=255) gAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAgg < 1:1021380/69‑1 (MQ=255) gAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAgg < 1:343134/69‑1 (MQ=255) gAATTTTTGCGTTGCGTAGCCGGCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAgg < 1:2075375/69‑1 (MQ=255) tgtgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2647594/66‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:869080/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:697937/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2313475/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:1017530/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:65872/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3380199/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2977069/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:335326/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3144948/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3179502/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3223025/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:3234362/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2270104/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:2166252/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:1125598/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:192655/69‑1 (MQ=255) tgcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:145483/69‑1 (MQ=255) tgcgtAGCCGTCGCTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:1178663/69‑1 (MQ=255) gcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:550011/68‑1 (MQ=255) gcgtAGCCGTCGGTACTGGAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACttt < 1:631914/68‑1 (MQ=255) gcgtAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTg > 1:3060091/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCTTTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCgc > 1:620848/1‑67 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:696079/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:2014728/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:1216823/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:1515753/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:319390/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:250882/1‑69 (MQ=255) aGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGAc > 1:1868940/1‑69 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1245649/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:529168/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1193483/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1757760/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:3126507/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1008402/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:293543/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:200293/69‑1 (MQ=255) tACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTACTCGACGt < 1:2571563/69‑1 (MQ=255) aCTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt < 1:1387042/68‑1 (MQ=255) cTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGtt > 1:899636/1‑68 (MQ=255) cTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGtt > 1:193140/1‑68 (MQ=255) cTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGtt > 1:1965963/1‑68 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTTGGATCGCTGACTTTg > 1:834786/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAgg > 1:662616/1‑39 (MQ=38) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1919040/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:2055972/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:2022502/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1337653/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:432295/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:387248/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1452030/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:2765329/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1569004/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:3195544/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:2820069/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTGAAGTAGCTAGGATCGCTGACTTTg > 1:1770557/1‑52 (MQ=255) ggAACCGTATTTGTTATCGAAGTCATTCAAGTAGCTAGGATCGCAGAct > 1:2600137/1‑48 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGa < 1:2698557/68‑1 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGa < 1:132714/68‑1 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGa < 1:2525945/68‑1 (MQ=255) ggAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGAGCGCTGACCTTGGTGTAGTCGACGTTGa < 1:3141628/68‑1 (MQ=255) ccGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGc < 1:2248658/69‑1 (MQ=255) ccGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGc < 1:2608109/69‑1 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGt > 1:806029/1‑56 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:1606981/1‑69 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:1836879/1‑69 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:2069906/1‑69 (MQ=255) cTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCacac > 1:1378874/1‑69 (MQ=255) | GAAGTTTTGCACCGCATAGCCAACGCTGAATTTTTGCGTTGCGTAGCCGTCAGTACTGGAACCGTACTTGTTATCGAAATCATTGAAGTAGCTAGGATCGCTGACCTTGGTGTAGTCGACGTTGAAACGCCACAC > minE/53684‑53818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |