| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 451,488 | C→T | 21.8% | A182A (GCG→GCA) | kdpA ← | potassium translocating ATPase, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 451,488 | 0 | C | T | 21.8% | 143.2 / 28.1 | 100 | A182A (GCG→GCA) | kdpA | potassium translocating ATPase, subunit A |
| Reads supporting (aligned to +/- strand): ref base C (35/43); new base T (13/9); total (48/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
CACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGCGTGCT > minE/451422‑451553 | cacaGCCTGATAAGGCAGAAAGTTTTGCAGCGCTCCTTGTTGAATAAAAAACAGTGCAATCAACAACGc > 1:869987/1‑69 (MQ=255)cacaGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGc > 1:282951/1‑69 (MQ=255)cacaGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGc > 1:2913638/1‑69 (MQ=255)cacaGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGc > 1:2506091/1‑69 (MQ=255) caGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGcc < 1:1124503/68‑1 (MQ=255) caGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGcc < 1:1163220/68‑1 (MQ=255) caGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGcc < 1:1553304/68‑1 (MQ=255) cTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAgg < 1:1006089/69‑1 (MQ=255) cTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAgg < 1:1958649/69‑1 (MQ=255) cTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAgg < 1:194349/69‑1 (MQ=255) cTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAgg < 1:2779591/69‑1 (MQ=255) cTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAgg < 1:2927979/69‑1 (MQ=255) cTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAgg < 1:2954039/69‑1 (MQ=255) cTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAgg < 1:1543022/69‑1 (MQ=255) cTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAgg < 1:3264815/69‑1 (MQ=255) cTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAgg < 1:2192352/69‑1 (MQ=255) tGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAggg < 1:710893/69‑1 (MQ=255) tGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAggg < 1:3102529/69‑1 (MQ=255) tGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAggg < 1:1658180/69‑1 (MQ=255) tGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAggg < 1:2132061/69‑1 (MQ=255) gATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAggg < 1:1214799/68‑1 (MQ=255) aaGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGTCt > 1:2429/1‑68 (MQ=255) aaGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACt > 1:3025430/1‑68 (MQ=255) aaGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACt < 1:2176875/68‑1 (MQ=255) aaGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACt < 1:2580740/68‑1 (MQ=255) gCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAgg < 1:1243494/61‑1 (MQ=255) gCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAg < 1:3046805/67‑1 (MQ=255) gCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAg < 1:2846916/67‑1 (MQ=255) gCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAg < 1:2882688/67‑1 (MQ=255) gCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAg < 1:2479046/67‑1 (MQ=255) gCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCa > 1:602376/1‑69 (MQ=255) gCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCa > 1:1682277/1‑69 (MQ=255) gCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCa > 1:2636659/1‑69 (MQ=255) gCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCa > 1:2059857/1‑69 (MQ=255) gCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCa > 1:1982245/1‑69 (MQ=255) gCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCa > 1:774006/1‑69 (MQ=255) gCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCa > 1:152871/1‑69 (MQ=255) gCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCa > 1:418051/1‑69 (MQ=255) cAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAgg > 1:2584942/1‑60 (MQ=255) gTTTTGCAGCGCACCTTGTTGTATAAAAAACAGTGCGATCAACAATGCCACAGGGGCTAACACCCATaa < 1:577268/69‑1 (MQ=255) gTTTTGCAGCGCACCTTGTTGTATAAAAAACAGTGCGATCAACAATGCCACAGGGGCTAACACCCATaa < 1:218266/69‑1 (MQ=255) gTTTTGCAGCGCACCTTGTTGTATAAAAAACAGTGCGATCAACAATGCCACAGGGGCTAACACCCATaa < 1:571336/69‑1 (MQ=255) gTTTTGCAGCGCACCTTGTTGTATAAAAAACAGTGCGATCAACAATGCCACAGGGGCTAACACCCATaa < 1:3301808/69‑1 (MQ=255) gTTTTGCAGCGCACCTTGTTGTATAAAAAACAGTGCGATCAACAATGCCACAGGGGCTAACACCCATaa < 1:4566/69‑1 (MQ=255) gTTTTGCAGCGCACCTTGTTGTATAAAAAACAGTGCGATCAACAATGCCACAGGGGCTAACACCCATaa < 1:2393143/69‑1 (MQ=255) gTTTTGCAGCGCACCTTGTTGTATAAAAAACAGTGCGATCAACAATGCCACAGGGGCTAACACCCATaa < 1:2374971/69‑1 (MQ=255) gTTTTGCAGCGCACCTTGTTGTATAAAAAACAGTGCGATCAACAATGCCACAGGGGCTAACACCCATaa < 1:1102223/69‑1 (MQ=255) gTTTTGCAGCGCACCTTGTTGTATAAAAAACAGTGCGATCAACAATGCCACAGGGGCTAACACCCATaa < 1:719768/69‑1 (MQ=255) ttGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGcc < 1:688241/46‑1 (MQ=255) cTTGTTGAATAAAATACAGTGCAATCAACAACGCCACAggg < 1:1554421/41‑1 (MQ=255) ttgttgAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAg < 1:1739659/45‑1 (MQ=255) ttgttgAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:656512/69‑1 (MQ=255) ttgttgAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:744943/69‑1 (MQ=255) ttgttgAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:2966468/69‑1 (MQ=255) ttgttgAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:1576099/69‑1 (MQ=255) ttgttgAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:2565175/69‑1 (MQ=255) ttgttgAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:1191916/69‑1 (MQ=255) ttgttgAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:633449/69‑1 (MQ=255) cAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:623687/54‑1 (MQ=255) cAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:3275991/54‑1 (MQ=255) cAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:1216571/54‑1 (MQ=255) cAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:1668383/54‑1 (MQ=255) cAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:2221332/54‑1 (MQ=255) cAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:2748974/54‑1 (MQ=255) cAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:649172/54‑1 (MQ=255) cAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAg < 1:2107227/54‑1 (MQ=255) cgaTCAACAATGCCACAGGGGCTAACACCCATAACGTGATGCGt > 1:469492/3‑44 (MQ=37) cgaTCAACAATGCCACAGGGGCTAACACCCATAACGTGATGCGt > 1:1718880/3‑44 (MQ=37) cgaTCAACAATGCCACAGGGGCTAACACCCATAACGTGATGCGt > 1:401818/3‑44 (MQ=37) cgaTCAACAATGCCACAGGGGCTAACACCCATAACGTGATGCGt > 1:474377/3‑44 (MQ=37) cgaTCAACAATGCCACAGGGGCTAACACCCATAACGTGATGCGt > 1:5295/3‑44 (MQ=37) cgaTCAACAATGCCACAGGGGCTAACACCCATAACGTGATGCGt > 1:539216/3‑44 (MQ=37) cgaTCAACAATGCCACAGGGGCTAACACCCATAACGTGATGCGt > 1:1283635/3‑44 (MQ=37) cgaTCAACAATGCCACAGGGGCTAACACCCATAACGTGATGCGt > 1:833154/3‑44 (MQ=37) cgaTCAACAATGCCACAGGGGCTAACACCCATAACGTGATGCGt > 1:810623/3‑44 (MQ=37) cgaTCAACAATGCCACAGGGGCTAACACCCATAACGTGATGCGt > 1:2763259/3‑44 (MQ=37) cgaTCAACAATGCCACAGGGGCTAACACCCATAACGTGATGCGt > 1:1853756/3‑44 (MQ=37) cgaTCAACAATGCCACAGGGGCTAACACCCATAACGTGATGCGt > 1:1615103/3‑44 (MQ=37) cAATCAACAACGCCACAGGGACTAGCACCCATAACGt > 1:1012912/1‑37 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGt > 1:1290962/1‑37 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGt > 1:1309414/1‑37 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGt > 1:1334428/1‑37 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGt > 1:3029395/1‑37 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGt > 1:161906/1‑37 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGt > 1:1839605/1‑37 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGt > 1:2329595/1‑37 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGt > 1:2004867/1‑37 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGt > 1:2093905/1‑37 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCg > 1:3311202/1‑53 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGa > 1:2117784/1‑69 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGa > 1:2184529/1‑69 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGa > 1:1839305/1‑69 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGa > 1:268060/1‑69 (MQ=255) cAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGa > 1:3286042/1‑69 (MQ=255) caacGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGCGTGCt < 1:2077977/69‑1 (MQ=255) caacGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGCGTGCt < 1:241636/69‑1 (MQ=255) caacGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGCGTGCt < 1:756283/69‑1 (MQ=255) caacGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGCGTGCt < 1:3404993/69‑1 (MQ=255) caacGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGCGTGCt < 1:896898/69‑1 (MQ=255) aacGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGCGTGCt < 1:571078/68‑1 (MQ=255) | CACAGCCTGATAAGGCAGAAAGTTTTGCAGCGCACCTTGTTGAATAAAAAACAGTGCAATCAACAACGCCACAGGGACTAGCACCCATAACGTGATGCGTAGCAGATCGACCCAGGCATTCCCGAGCGTGCT > minE/451422‑451553 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |